Kluyveromyces lactis SSO1 and SEB1 genes are functional in Saccharomyces cerevisiae and enhance production of secreted proteins when overexpressed

Yeast

Volumn 21, Issue 12, 2004, Pages 1045-1055

  Toikkanen, Jaana H ^a   Sundqvist, Lena ^a   Keränen, Sirkka ^a  

^a VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

Author keywords

Complementation; Increased protein production; Kluyveromyces lactis; Multicopy suppression; Protein secretion; Saccharomyces cerevisiae; SEB1 SBH1; SSO genes

Indexed keywords

AMYLASE; DNA FRAGMENT; FUNGAL DNA; FUNGAL PROTEIN; POLYCLONAL ANTIBODY;

AMINO ACID SEQUENCE; ARTICLE; CANDIDA UTILIS; CELL LYSATE; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; EXOCYTOSIS; FUNGAL GENE; FUNGUS GROWTH; GENE OVEREXPRESSION; GENETIC COMPLEMENTATION; KLSEB1 GENE; KLSSO1 GENE; KLUYVEROMYCES; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SECRETION; SACCHAROMYCES CEREVISIAE; SEB1 GENE; SEQUENCE HOMOLOGY; SSO1 GENE; TEMPERATURE SENSITIVE MUTANT; YARROWIA LIPOLYTICA;

ALPHA-AMYLASE; AMINO ACID SEQUENCE; BASE SEQUENCE; BLOTTING, SOUTHERN; BLOTTING, WESTERN; CLONING, MOLECULAR; DNA, FUNGAL; ENDOPLASMIC RETICULUM; EXOCYTOSIS; FUNGAL PROTEINS; GENETIC COMPLEMENTATION TEST; KLUYVEROMYCES; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; PLASMIDS; SACCHAROMYCES CEREVISIAE; SEQUENCE ALIGNMENT;

CANDIDA; KLUYVEROMYCES; KLUYVEROMYCES LACTIS; PICHIA; PICHIA JADINII; PICHIA PASTORIS; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 6344238954     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/yea.1151     Document Type: Article

References (50)

1. Aalto MK, Ronne H, Keränen S. 1993. Yeast syntaxins Sso1p and Sso2p belong to a family of related membrane proteins that function in vesicular transport. EMBO J 12: 4095-4104.
2. Bartkevičiũtė D, Šiekštelė R, Sasnauskas K. 2000. Heterologous expression of the Kluyveromyces marxianus endopolygalacturonase gene (EPG1) using versatile autonomously replicating vector for a wide range of hosts. Enz Microbiol Technol 26: 653-656.
3. Boisramé A, Chasles M, Babour A, et al. 2002. Sbh1p, a subunit of the Sec61 translocon, interacts with the chaperone calnexin in yeast Yarrowia lipolytica. J Cell Sci. 115: 4947-4956.
4. Brummer MH, Richard P, Sundqvist L, et al. 2001a. GD11 genes from Kluyveromyces lactis and Pichia pastoris: cloning and functional expression in Saccharomyces cerevisiae. Yeast 18: 897-902.
5. Brummer MH, Kivinen KJ, Jäntti J, et al. 2001b. Characterization of the sec1-1 and sec1-11 mutations. Yeast 18: 1525-1536.
6. Corpet F. 1988. Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res. 16: 10 881-10 890.
7. Finger FP, Novick P. 1997. Sec3p is involved in secretion and morphogenesis in Saccharomyces cerevisiae. Mol Biol Cell 8: 647-662.
8. Finger FP, Hughes TE, Novick P. 1998. Sec3p is a spatial landmark for polarized secretion in budding yeast. Cell 92: 559-571.
9. Finke K, Plath K, Panzner S, et al. 1996. A second trimeric complex containing homologs of the Sec61p complex functions in protein transport across the ER membrane of S. cerevisiae. EMBO J 15: 1482-1494.
10. Gallwitz D, Jahn R. 2003. The riddle of the Sec1/Munc-18 proteins - new twists added to their interactions with SNAREs. Trends Biochem Sci 28: 113-116.
11. Gellissen G, Hollenberg CP. 1997. Application of yeasts in gene expression studies: a comparison of Saccharomyces cerevisiae, Hansenula polymorpha and Kluyveromyces lactis - a review. Gene 190: 87-97.
12. Gietz RD, Sugino A. 1988. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534.
13. Guo W, Roth D, Walch-Solimena C, Novick P. 1999. The exocyst is an effector for Sec4p, targeting secretory vesicles to sites of exocytosis. EMBO J 18: 1071-1080.
14. Guo W, Novick P. 2004. The exocyst meets the translocon: a regulatory circuit for secretion and protein synthesis? Trends Cell Biol 14: 61-63.
15. Haapa S, Taira S, Heikkinen E, Savilahti H. 1999a. An efficient and accurate integration of mini-Mu transposons in vitro: a general methodology for functional genetic analysis and molecular biology applications. Nucleic Acids Res 27: 2777-2784.
16. Haapa S, Suomalainen S, Eerikäinen S, et al. 1999b. An efficient DNA sequencing strategy based on the bacteriophage Mu in vitro DNA transposition reaction. Genome Res 9: 308-315.
17. Haapa-Paananen S, Rita H, Savilahti H. 2002. DNA transposition of bacteriophage Mu: a quantitative analysis of target site selection in vitro. J Biol Chem 277: 2843-2851.
18. Harmsen MM, Bruyne MI, Raué HA, Maat J. 1996. Overexpression of binding protein and disruption of the PMR1 gene synergistically stimulate secretion of bovine prochymosin but not plant Thaumatin in yeast. Appl Microbiol Biotechnol 46: 365-370.
19. Hofmann K, Stoffel W. 1993. TMbase - a database of membrane spanning protein segments. Biol Chem Hoppe-Seyler 374: 166.
20. Jäntti J, Keränen S, Toikkanen J, et al. 1994. Membrane insertion and intracellular transport of yeast syntaxin Sso2p in mammalian cells. J Cell Sci 107: 3623-3633.
21. Jäntti J, Lahdenranta J, Olkkonen VM, et al. 1999. SEM1, a homologue of the Split hand/split foot malformation candidate gene Dss1, regulates exocytosis and pseudohyphal differentiation in yeast. Proc Natl Acad Sci USA 96: 909-914.
22. Jäntti J, Aalto MK, Öyen M, et al. 2002. Characterization of temperature-sensitive mutations in the yeast syntaxin1 homologues Sso1p and Sso2p, and evidence of a distinct function for Sso1p in sporulation. J Cell Sci 115: 409-420.
23. Kaiser CA, Michaelis S, Mitchell A. 1994. Methods in Yeast Genetics. Cold Spring Harbor Laboratory Press: New York.
24. Kauppi M, Jäntti J, Olkkonen VM. 2004. The function of Sec1/Munc 18 proteins - solution of the mystery in sight? Top Curr Genet 10: 115-143.
25. Kiers J, Zeeman AM, Luttik M, et al. 1998. Regulation of alcoholic fermentation in batch and chemostat cultures of Kluyveromyces lactis CBS 2359. Yeast 14: 459-469.
26. Laemmli UK. 1970. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227: 680-685.
27. Larsson S, Cassland P, Jönsson LJ. 2001. Development of a Saccharomyces cerevisiae strain with enhanced resistance to phenolic fermentation inhibitors in lignocellulose hydrolysates by heterologous expression of laccase. Appl Environm Microbiol 67: 1163-1170.
28. Lipschutz JH, Lingappa VR, Mostov KE. 2003. The exocyst affects protein synthesis by acting on the translocation machinery of the endoplasmic reticulum. J Biol Chem 278: 20 954-20 960.
29. Mizuta K, Warner JR. 1994. Continued functioning of the secretory pathway is essential for ribosome synthesis. Mol Cell Biol 14: 2493-2502.
30. Neiman AM. 1998. Prospore formation defines a developmentally regulated branch of the secretory pathway in yeast. J Cell Biol 140: 29-37.
31. Panzner S, Dreier L, Hartmann E, et al. 1995. Posttranslational protein transport in yeast reconstituted with a purified complex of Sec proteins and Kar2p. Cell 81: 561-570.
32. Pearson WR, Wood T, Zhang Z, Miller W. 1997. Comparison of DNA sequences with protein sequences. Genomics 46: 24-36.
33. Prior C, Mamessier P, Fukuhara H, et al. 1993. The hexokinase gene is required for transcriptional regulation of the glucose transporter gene RAG1 in Kluyveromyces lactis. Mol Cell Biol 13: 3882-3889.
34. Robinson AS, Hines V, Wittrup KD. 1994. Protein disulfide isomerase overexpression increases secretion of foreign proteins in Saccharomyces cerevisiae. Bio/Technol 12: 381-384.
35. Ruohonen L, Hackman P, Lehtovaara P, et al. 1987. Efficient secretion of Bacillus amyloliquefaciens α-amylase by its own signal peptide from Saccharomyces cerevisiae host cells. Gene 59: 161-170.
36. Ruohonen L, Penttä M, Keränen S. 1991. Optimization of Bacillus α-amylase production by Saccharomyces cerevisiae. Yeast 7: 337-346.
37. Ruohonen L, Aalto MK, Keränen S. 1995. Modifications to the ADH1 promoter of Saccharomyces cerevisiae for efficient production of heterologous proteins. J Biotechnol 39: 193-203.
38. Ruohonen L, Toikkanen J, Tieaho V, et al. 1997. Enhancement of protein secretion in Saccharomyces cerevisiae by overproduction of Sso protein, a late-acting component of the secretory machinery. Yeast 13: 337-351.
39. Salminen A, Novick P. 1987. A ras-like protein is required for a post-Golgi event in yeast secretion. Cell 49: 527-538.
40. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press: New York.
41. Schägger H, von Jagow G. 1987. Tricine-sodium dodecyl sulphate polyacrylamide gel electrophoresis for separation of proteins in the range 1-100 kDa. Anal Biochem 166: 368-379.
42. Sherman F, Fink G, Hicks JB. 1989. Methods in Yeast Genetics. Cold Spring Harbor Laboratory Press: New York.
43. Shusta EV, Raines RT, Plückthun A, Wittrup KD. 1998. Increasing the secretory capacity of Saccharomyces cerevisiae for production of single-chain antibody fragments. Nature Biotechnol 16: 773-777.
44. Sikorski RS, Hieter P. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation DNA in Saccharomyces cerevisiae. Genetics 122: 19-27.
45. Swinkels BW, van Ooyen AJJ, Bonekamp FJ. 1993. The yeast Kluyvervmyces lactis as an efficient host for heterologous gene expression. Antonie van Leeuwenhoek 64: 187-201.
46. TerBush DR, Maurice T, Roth D, Novick P. 1996. The exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae. EMBO J 15: 6483-6494.
47. Toikkanen J, Gatti E, Takei K, et al. 1996. Yeast protein translocation complex: isolation of two genes, SEB1 and SEB2, encoding proteins homologous to the Sec61β subunit. Yeast 12: 425-438.
48. Toikkanen JH, Miller KJ, Söderlund H, et al. 2003. The β subunit of the Sec61p ER translocon interacts with exocyst complex in Saccharomyces cerevisiae. J Biol Chem 278: 20 946-20 953.
49. Valkonen M, Penttilä M, Saloheimo M. 2003. Effects of inactivation and constitutive expression of the unfolded-protein response pathway on protein production in the yeast Saccharomyces cerevisiae. Appl Environm Microbiol 69: 2065-2072.
50. Vernet T, Dignard D, Thomas DY. 1987. A family of yeast expression vectors containing the phage fl intergenic region. Gene 52: 225-233.