Metabolic flux analysis of recombinant Pichia pastoris growing on different glycerol/methanol mixtures by iterative fitting of NMR-derived 13C-labelling data from proteinogenic amino acids

New Biotechnology

Volumn 31, Issue 1, 2014, Pages 120-132

  Jordà, Joel ^a   De Jesus, Sérgio S ^b   Peltier, Solenne ^c   Ferrer, Pau ^a   Albiol, Joan ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^b UNIVERSITY OF CAMPINAS   (Brazil)

^c UNIVERSITÉ CLERMONT AUVERGNE   (France)

Author keywords

KG; 13dpg; ACALD; ACCoA; BFD; CER; CGly; CHOR; CIT; DHA; DHAP; DHF; E4P; ETC; FBP; FOR; Form; Fru6P; FullyGly; FUM; G1P: SUCCoA; GA3P; GA3Pper; Glc6P; ICIT; Kval; MAL; Metoh; MetohL; MetohN; MTHF; N.d.; NMR; OAA; OUR; Pep; PG2; PG3; Pi; PPi; PPP; PRPP; Pyr; Rib5P; ROL; Rul5P; Sd; Sed7P; Sem; SUCC; THF; Xbio; Xul5P

Indexed keywords

13DPG; ACALD; ACCOA; BFD; CER; CGLY; CHOR; CIT; DHA; DHAP; DHF; E4P; ETC; FBP; FOR; FRU6P; FULLYGLY; FUM; G1P: SUCCOA; GA3P; GA3PPER; GLC6P; ICIT; KVAL; MAL; METOH; METOHL; METOHN; MTHF; OAA; OUR; PEP; PG2; PG3; PPI; PPP; PRPP; PYR; RIB5P; ROL; RUL5P; SD; SED7P; SUCC; THF; XBIO; XUL5P;

AMINO ACIDS; CARBON; CARBON DIOXIDE; CULTIVATION; FORMING; GROWTH RATE; METHANOL; MIXTURES; NUCLEAR MAGNETIC RESONANCE; PHYSIOLOGY; POLYIMIDES; PROTEINS; SCANNING ELECTRON MICROSCOPY; YEAST;

METABOLISM;

AMINO ACID; CARBON 13; GLYCEROL; METHANOL; RECOMBINANT PROTEIN; TRIACYLGLYCEROL LIPASE;

AMINO ACID COMPOSITION; ARTICLE; BIOMASS; BIOREACTOR; CARBON METABOLISM; CARBON NUCLEAR MAGNETIC RESONANCE; CARBON SOURCE; CHEMOSTAT; CITRIC ACID CYCLE; DILUTION; FUNGAL METABOLISM; FUNGUS CULTURE; GLUCONEOGENESIS; GROWTH RATE; ISOTOPE LABELING; LIMIT OF DETECTION; MINERAL BALANCE; MITOCHONDRION; NONHUMAN; OXIDATIVE PHOSPHORYLATION; OXYGEN CONSUMPTION; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN SECRETION; RESPIRATORY CHAIN; STOICHIOMETRY; STRESS;

PICHIA PASTORIS;

1,3-DIPHOPHOGLYCERATE; 13DPG; 2-PHOSPHOGLYCERATE; 3-PHOSPHOGLYCERATE; ACALD; ACCOA; ACETALEHYDE; ACETYL COENZYME; BFD; BIOMASS FORMATION; BIOSYNTHETICALLY DIRECTED FRACTIONAL; CER; CGLY; CHOR; CHORISMATE; CIT; CITRATE; CO(2) EXCHANGE RATE; DHA; DHAP; DHF; DIHYDROFOLATE; DIHYDROXYACETONE; DIHYDROXYACETONE PHOSPHATE; E4P; ELECTRON TRANSFER CHAIN; ERITROSE-4-PHOSPHATE; ETC; FBP; FOR; FORM; FORMALDEHYDE; FORMATE; FRU6P; FRUCTOSE-1,6-BIPHOSPHATE; FRUCTOSE-6-PHOSPHATE; FULLY LABELLED GLYCEROL; FULLYGLY; FUM; FUMARATE; G1P: SUCCOA; GA3P; GA3P(PER); GLC6P; GLUCOSE-6-PHOSPHATE; GLYCERALDEHYDE-3-PHOSPHATE; GLYCERALDEHYDE-3-PHOSPHATE PEROXISOME; ICIT; INORGANIC PHOSPHATE; INORGANIC PYROPHOSPHATE; ISOCITRATE; KETOVALERATE; KVAL; LABELLED METHANOL; MAL; MALATE; METHANOL; METHYLENETETRAHYDROFOLATE; METOH; METOHL; METOHN; MTHF; N.D.; NMR; NON LABELLED METHANOL; NON-LABELLED GLYCEROL; NOT DETERMINED; NUCLEAR MAGNETIC RESONANCE; OAA; OUR; OXALOACETATE; OXIGEN UPTAKE RATE; PENTOSE PHOSPHATE PATHWAY; PEP; PG2; PG3; PHOSPHOENOLPYRUVATE; PHOSPHORIBOSYL PIROPHOSPHATE; PI; PPI; PPP; PRPP; PYR; PYRUVATE; RHIZOPUS ORYZAE LIPASE; RIB5P; RIBOSE-5-PHOSPHATE; RIBULOSE-5-PHOSPHATE; ROL; RUL5P; SD; SED7P; SEDOHEPTULOSE-7-PHOSPHATE; SEM; STANDARD DEVIATION; STANDARD ERROR OF THE MEAN; SUCC; SUCCINATE; SUCCINYL COENZYME A; TETRAHYDROFOLATE; THF; X(BIO); XUL5P; XYLULOSE-5-PHOSPHATE; Α-KETOGLUTARATE; ΑKG;

CARBON ISOTOPES; GLUCOSE; GLYCEROL; MAGNETIC RESONANCE SPECTROSCOPY; METHANOL; PICHIA; RECOMBINANT PROTEINS; SOLVENTS;

EID: 84889604461     PISSN: 18716784     EISSN: 18764347     Source Type: Journal    
DOI: 10.1016/j.nbt.2013.06.007     Document Type: Article

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