Native-State stability determines the extent of degradation relative to secretion of protein variants from Pichia pastoris

PLoS ONE

Volumn 6, Issue 7, 2011, Pages

  Whyteside, Graham ^a   Alcocer, Marcos J C ^a   Kumita, Janet R ^b   Dobson, Christopher M ^b   Lazarou, Maria ^a,c   Pleass, Richard J ^a,c   Archer, David B ^a  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^c LIVERPOOL SCHOOL OF TROPICAL MEDICINE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

LYSOZYME; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; FUNGAL PROTEIN; MESSENGER RNA; MUTANT PROTEIN;

AMYLOIDOSIS; ARTICLE; CONTROLLED STUDY; ENZYME REGULATION; NONHUMAN; PICHIA PASTORIS; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN SECRETION; PROTEIN STABILITY; PROTEIN VARIANT; UNFOLDED PROTEIN RESPONSE; AUTOPHAGY; CHEMISTRY; ENDOPLASMIC RETICULUM ASSOCIATED DEGRADATION; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION REGULATION; GENETICS; HUMAN; INTRACELLULAR SPACE; METABOLISM; PICHIA; SECRETION;

PICHIA PASTORIS;

AUTOPHAGY; ENDOPLASMIC RETICULUM-ASSOCIATED DEGRADATION; ENZYME-LINKED IMMUNOSORBENT ASSAY; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; HUMANS; INTRACELLULAR SPACE; MURAMIDASE; MUTANT PROTEINS; PICHIA; PROTEIN STABILITY; RNA, MESSENGER; SINGLE-CHAIN ANTIBODIES; UNFOLDED PROTEIN RESPONSE;

EID: 79960867411     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0022692     Document Type: Article

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