Cloning, disruption and protein secretory phenotype of the GAS1 homologue of Pichia pastoris

FEMS Microbiology Letters

Volumn 264, Issue 1, 2006, Pages 40-47

  Marx, Hans ^a   Sauer, Michael ^a,b,e   Resina, David ^c   Vai, Marina ^d   Porro, Danilo ^d   Valero, Francisco ^c   Ferrer, Pau ^c   Mattanovich, Diethard ^a,b  

^a UNIVERSITY OF AGRICULTURAL SCIENCES   (Austria)

^b Fachhochschule Campus Wien   (Austria)

^c UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^d UNIVERSITY OF MILANO BICOCCA   (Italy)

^e NONE   (Austria)

Author keywords

1,3 glucanosyltransglycosylase; Cell wall; Gene disruption; Heterologous protein secretion; Pichia pastoris; Yeast

Indexed keywords

FUNGAL PROTEIN; GLYCOPHOSPHOLIPID ANCHORED SURFACE PROTEIN 1; HUMAN SERUM ALBUMIN; PHOSPHORYLASE; RECOMBINANT PROTEIN; TRIACYLGLYCEROL LIPASE; TRYPSINOGEN; UNCLASSIFIED DRUG;

ARTICLE; FUNGUS MUTANT; GENETIC CODE; MOLECULAR CLONING; MORPHOLOGY; NONHUMAN; NUCLEOTIDE SEQUENCE; NULL ALLELE; PHENOTYPE; PICHIA PASTORIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RHIZOPUS ORYZAE; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; FUNGAL PROTEINS; GENE DELETION; GENETIC COMPLEMENTATION TEST; GLYCOSYLTRANSFERASES; HUMANS; LIPASE; MEMBRANE GLYCOPROTEINS; MOLECULAR SEQUENCE DATA; PHENOTYPE; PICHIA; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS; RHIZOPUS; SACCHAROMYCES CEREVISIAE; SEQUENCE ALIGNMENT; SERUM ALBUMIN; TRYPSINOGEN;

PICHIA PASTORIS; RHIZOPUS ORYZAE; SACCHAROMYCES CEREVISIAE;

EID: 33749433359     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2006.00427.x     Document Type: Article

References (27)

1. Carotti C, Ragni E, Palomares O, Fontaine T, Tedeschi G, Rodriguez R, Latge JP, Vai M & Popolo L (2004) Characterization of recombinant forms of the yeast Gas1 protein and identification of residues essential for glucanosyltransferase activity and folding. Eur J Biochem 271: 3635-3645.
2. Cereghino JL & Cregg JM (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 24: 45-66.
3. Cos O, Serrano A, Montesinos JL, Ferrer P, Cregg JM & Valero F (2005) Combined effect of the methanol utilization (Mut) phenotype and gene dosage on recombinant protein production in Pichia pastoris fed-batch cultures. J Biotechnol 116: 321-335.
4. Ferrer P, Diers I, Asenjo JA & Andrews BA (1998) Yeast cell permeabilizing beta-1,3-glucanases: a tool for the integration of downstream processes and metabolic engineering applications to yeast. Biotechnol Bioeng 58: 321-324.
5. Gasser B, Maurer M, Gach J, Kunert R & Mattanovich D (2006) Engineering of Pichia pastoris for improved production of antibody fragments. Biotechnol Bioeng 94: 353-361.
6. Gietz RD & Woods RA (2002) Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method. Methods Enzymol 350: 87-96.
7. Hoffman CS & Winston F (1987) A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene 57: 267-272.
8. Hohenblum H, Borth N & Mattanovich D (2003) Assessing viability and cell-associated product of recombinant protein producing Pichia pastoris with flow cytometry. J Biotechnol 102: 281-290.
9. Hohenblum H, Gasser B, Maurer M, Borth N & Mattanovich D (2004) Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris. Biotechnol Bioeng 85: 367-375.
10. Lesage G, Shapiro J, Specht CA, Sdicu AM, Menard P, Hussein S, Tong AH, Boone C & Bussey H (2005) An interactional network of genes involved in chitin synthesis in Saccharomyces cerevisiae. BMC Genet 6: 8.
11. Mori K, Sant A, Kohno K, Normington K, Gething M-J & Sambrook JF (1992) A 22bp cis-acting element is necessary and sufficient for the induction of the yeast KAR2 (BiP) gene by unfolded proteins. EMBO J 11: 2583-2593.
12. Mouyna I, Fontaine T, Vai M, Monod M, Fonzi WA, Diaquin M, Popolo L, Hartland RP & Latge JP (2000a) Glycosylphosphatidylinositol-anchored glucanosyltransferases play an active role in the biosynthesis of the fungal cell wall. J Biol Chem 275: 14882-14889.
13. Mouyna I, Monod M, Fontaine T, Henrissat B, Lechenne B & Latge JP (2000b) Identification of the catalytic residues of the first family of beta(1-3)glucanosyltransferases identified in fungi. Biochem J 347: 741-747.
14. Mouyna I, Morelle W, Vai M et al. (2005) Deletion of GEL2 encoding for a beta(1-3)glucanosyltransferase affects morphogenesis and virulence in Aspergillus fumigatus. Mol Microbiol 56: 1675-1688.
15. Papaleo E, Fantucci P, Vai M & De Gioia L (2006) Three-dimensional structure of the catalytic domain of the yeast beta-(1,3)-glucan transferase Gas1: a molecular modeling investigation. J Mol Model (Online) 12: 237-248.
16. Popolo L & Vai M (1999) The Gas1 glycoprotein, a putative wall polymer cross-linker. Biochim Biophys Acta 1426: 385-400.
17. Popolo L, Vai M, Gatti E, Porello S, Bonfante P, Balestrini R & Alberghina L (1993) Physiological analysis of mutants indicates involvement of the Saccharomyces cerevisiae GPI-anchored protein gp115 in morphogenesis and cell separation. J Bacteriol 175: 1879-1885.
18. Porro D, Sauer M, Branduardi P & Mattanovich D (2005) Recombinant protein production in yeasts. Mol Biotechnol 31: 245-259.
19. Resina D, Serrano A, Valero F & Ferrer P (2004) Expression of a Rhizopus oryzae lipase in Pichia pastoris under control of the nitrogen source-regulated formaldehyde dehydrogenase promoter. J Biotechnol 109: 103-113.
20. Resina D, Cos O, Ferrer P & Valero F (2005) Developing high cell density fed-batch cultivation strategies for heterologous protein production in Pichia pastoris using the nitrogen source-regulated FLD1 Promoter. Biotechnol Bioeng 91: 760-767.
21. Rossini D, Porro D, Brambilla L, Venturini M, Ranzi BM, Vanoni M & Alberghina L (1993) In Saccharomyces cerevisiae, protein secretion into the growth medium depends on environmental factors. Yeast 9: 77-84.
22. Sreekrishna K, Brankamp RG, Kropp KE, Blankenship DT, Tsay JT, Smith PL, Wierschke JD, Subramaniam A & Birkenberger LA (1997) Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris. Gene 190: 55-62.
23. Vai M, Orlandi I, Cavadini P, Alberghina L & Popolo L (1996) Candida albicans homologue of GGP1/GAS1 gene is functional in Saccharomyces cerevisiae and contains the determinants for glycosylphosphatidylinositol attachment. Yeast 12: 361-368.
24. Vai M, Brambilla L, Orlandi I, Rota N, Ranzi BM, Alberghina L & Porro D (2000) Improved secretion of native human insulin-like growth factor 1 from gas1 mutant Saccharomyces cerevisiae cells. Appl Environ Microbiol 66: 5477-5479.
25. Valkonen M, Penttila M & Saloheimo M (2003) Effects of inactivation and constitutive expression of the unfolded-protein response pathway on protein production in the yeast Saccharomyces cerevisiae. Appl Environ Microbiol 69: 2065-2072.
26. Venturini M, Morrione A, Pisarra P, Martegani E & Vanoni M (1997) In Saccharomyces cerevisiae a short amino acid sequence facilitates excretion in the growth medium of periplasmic proteins. Mol Microbiol 23: 997-1007.
27. Wach A, Brachat A, Pohlmann R & Philippsen P (1994) New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10: 1793-1808.