Engineering of a Pichia pastoris expression system for secretion of high amounts of intact human parathyroid hormone

Journal of Biotechnology

Volumn 116, Issue 3, 2005, Pages 251-260

  Vad, Randi ^a   Nafstad, Eidi ^a   Dahl, Linn Anita ^a   Gabrielsen, Odd S ^a  

^a UNIVERSITY OF OSLO   (Norway)

Author keywords

EDTA; Heterologous protein expression; Human parathyroid hormone; Pichia pastoris; Protein disulphide isomerase; Ubiquitin

Indexed keywords

BONE; CALCIUM; ENZYMES; GROWTH KINETICS; PATIENT TREATMENT; YEAST;

BONE GROWTH; CALCIUM METABOLISM; OSTEOPOROSIS; PICHIA PASTORIS;

HORMONES;

CYSTEINE; EDETIC ACID; METALLOPROTEINASE; PARATHYROID HORMONE; SACCHAROMYCES CEREVISIAE PROTEIN; SACCHAROMYCES CEREVISIAE PROTEIN DISULFIDE ISOMERASE; UBIQUITIN; UNCLASSIFIED DRUG;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME INDUCTION; ENZYME SYNTHESIS; FUNGUS CULTURE; GENE EXPRESSION SYSTEM; GENETIC ENGINEERING; NONHUMAN; PARATHYROID HORMONE RELEASE; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN PROTEIN INTERACTION; PROTEIN STABILITY; SACCHAROMYCES CEREVISIAE; YEAST;

CELL CULTURE TECHNIQUES; EDETIC ACID; FEASIBILITY STUDIES; GENE EXPRESSION REGULATION, FUNGAL; HUMANS; PARATHYROID HORMONE; PICHIA; PROTEIN DISULFIDE-ISOMERASE; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE;

PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 13544253581     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2004.12.004     Document Type: Article

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