Investigating the physiological response of Pichia (Komagataella) pastoris GS115 to the heterologous expression of misfolded proteins using chemostat cultures

Applied Microbiology and Biotechnology

Volumn 97, Issue 22, 2013, Pages 9747-9762

  Hesketh, Andrew R ^a,b   Castrillo, Juan I ^a,b   Sawyer, Trevor ^a   Archer, David B ^c   Oliver, Stephen G ^a,b  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^c UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

Chemostat; Endoplasmic reticulum (ER) stress; Heterologous protein production; Komagataella pastoris; Pichia pastoris; RNA Seq; Unfolded protein response (UPR)

Indexed keywords

ENDOPLASMIC RETICULUM STRESS; HETEROLOGOUS PROTEIN PRODUCTION; PASTORIS; PICHIA PASTORIS; RNA-SEQ; UNFOLDED PROTEIN RESPONSE;

BIOSYNTHESIS; CELL MEMBRANES; CYTOLOGY; ENZYMES; METHANOL; NUCLEIC ACIDS; PHYSIOLOGICAL MODELS; PHYSIOLOGY; RECOMBINANT PROTEINS; YEAST;

CHEMOSTATS;

AMYLOID PROTEIN; LYSOZYME; TRANSCRIPTOME;

CHEMOSTAT; FUNGUS; GENE EXPRESSION; GENOME; GROWTH RATE; NUTRIENT DYNAMICS; PHYSIOLOGICAL RESPONSE; PROTEIN; RECOMBINATION; RNA; STEADY-STATE EQUILIBRIUM;

ARTICLE; BACTERIAL GENOME; CELL AGGREGATION; CELL FUNCTION; CHEMOSTAT; CULTURE TECHNIQUE; GENETIC TRANSCRIPTION; GROWTH RATE; HETEROLOGOUS EXPRESSION; NONHUMAN; PEPTIDE SYNTHESIS; PHYSIOLOGICAL PROCESS; PICHIA PASTORIS; PROTEIN FOLDING; STEADY STATE; UNFOLDED PROTEIN RESPONSE;

PICHIA PASTORIS;

EID: 84888441022     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-5186-1     Document Type: Article

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