Single-chain antibody fragment production in Pichia pastoris: Benefits of prolonged pre-induction glycerol feeding

Biotechnology Journal

Volumn 6, Issue 4, 2011, Pages 452-462

  Khatri, Narendar K ^a,b   Gocke, Dörte ^a,e   Trentmann, Oliver ^c,f   Neubauer, Peter ^b,d   Hoffmann, Frank ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^b UNIVERSITY OF OULU   (Finland)

^c Novoplant GmbH   (Germany)

^d TECHNISCHE UNIVERSITÄT BERLIN   (Germany)

^e FORSCHUNGSZENTRUM JÜLICH   (Germany)

^f Rheinland Pfälzische Technische Universität Kaiserslautern Landau   (Germany)

Author keywords

AOX1 promoter; Pichia pastoris; Process optimization; Recombinant protein production; Single chain antibody fragment

Indexed keywords

AOX1 PROMOTER; PICHIA PASTORIS; PROCESS OPTIMIZATION; RECOMBINANT PROTEIN PRODUCTION; SINGLE-CHAIN ANTIBODY FRAGMENT;

ANTIBODIES; FEEDING; GENE EXPRESSION; MACHINERY; METHANOL; NUCLEIC ACIDS; OPTIMIZATION; OXYGEN; PHYSIOLOGY; PROCESS CONTROL;

GLYCEROL;

GLYCEROL; MESSENGER RNA; METHANOL; OXYGEN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY PRODUCTION; ARTICLE; CELL DENSITY; CULTURE MEDIUM; DOWNSTREAM PROCESSING; INCUBATION TIME; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SUPERNATANT; UNFOLDED PROTEIN RESPONSE;

BIOREACTORS; BIOTECHNOLOGY; GLYCEROL; PICHIA; SINGLE-CHAIN ANTIBODIES;

PICHIA PASTORIS;

EID: 79953681894     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201000193     Document Type: Article

References (35)

1. Werten, M. W. T., van den Bosch, T. J., Wind, R. D., Mooibroek, H., de Wolf, F. A., High-yield secretion of recombinant gelatins by Pichia pastoris. Yeast 1999, 15, 1087-1096.
2. Damasceno, L. M., Pla, I., Chang, H.-J., Cohen, L. et al., An optimized fermentation process for high-level production of a single-chain Fv antibody fragment in Pichia pastoris. Protein Expr. Purif. 2004, 37, 18-26.
3. Freyre, F. M., Vázquez, J. E., Ayala, M., Canaán-Haden, L. et al., Very high expression of an anti-carcinoembryonic antigen single chain Fv antibody fragment in the yeast Pichia pastoris. J. Biotechnol. 2000, 76, 157-163.
4. Lin Cereghino, G. P., Lin Cereghino, J., Ilgen, C., Cregg, J. M., Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr. Opin. Biotechnol. 2002, 13, 329-332.
5. Yurimoto, H., Sakai, Y., Kato, N., Methanol metabolism, in: Gellisen G. (Ed.), Hansenula polymorpha. Biology and Applications, Wiley-VCH, Weinheim 2002, pp. 61-75.
6. Bushell, M. E., Rowe, M., Avignone-Rossa, C. A., Wardell, J. N., Cyclic fed-batch culture for production of human serum albumin in Pichia pastoris. Biotechnol. Bioeng. 2003, 82, 678-683.
7. Lee, C. Y., Lee, S. J., Jung, K. H., Katoh, S., Lee, E. K., High dissolved oxygen tension enhances heterologous protein expression by recombinant Pichia pastoris. Proc. Biochem. 2003, 38, 1147-1154.
8. Lin Cereghino, J., Cregg, J. M., Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 2000, 24, 45-66.
9. Thiry, M., Cingolani, D., Optimizing scale-up fermentation processes. Trends Biotechnol. 2002, 20, 103-105.
10. Curvers, S., Brixius, P., Klauser, T., Thömmes, J. et al., Human chymotrypsinogen B production with Pichia pastoris by integrated development of fermentation and downstream processing. Part 1. Fermentation. Biotechnol. Prog. 2001, 17, 495-502.
11. Stratton, J., Chiruvolu, V., Meagher, M., High cell-density fermentation, in: Higgins D.R., Cregg J.M. (Eds.), Pichia Protocols, Humana Press, Totowa 1998, pp. 107-120.
12. Trentmann, O., Khatri, N. K., Hoffmann, F., Reduced oxygen supply increases process stability and product yield with recombinant Pichia pastoris. Biotechnol. Prog. 2004, 20, 1766-1775.
13. Zhou, X.-S., Zhang, Y.-X., Decrease of proteolytic degradation of recombinant hirudin produced by Pichia pastoris by controlling the specific growth rate. Biotechnol. Lett. 2002, 24, 1449-1453.
14. Jahic, M., Wallberg, F., Bollok, M., Garcia, P., Enfors, S.-O., Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures. Microbial Cell Fact. 2003, 2, 6.
15. Qureshi, M. S., Zhang, D., Du, G., Chen, J., Improved production of polygalacturonate lyase by combining a pH and online methanol control strategy in a two-stage induction phase with a shift in the transition phase. J. Ind. Microbiol. Biotechnol. 2010, 37, 323-333.
16. Minning, S., Serrano, A., Ferrer, P., Solá, C., Schmid, R. D., Valero, F., Optimization of high-level production of Rhizopus oryzae lipase in Pichia pastoris. J. Biotechnol. 2001, 86, 59-70.
17. Hellwig, S., Emde, F., Raven, N. P. G., Henke, M., van der Logt, P., Fischer, R., Analysis of single-chain antibody production in Pichia pastoris using on-line methanol control in fed-batch and mixed-feed fermentations. Biotechnol. Bioeng. 2001, 74, 344-352.
18. Korz, D. J., Rinas, U., Hellmuth, K., Sanders, E. A., Deckwer, W. D., Simple fed-batch technique for high cell density cultivation of Escherichia coli. J. Biotechnol. 1995, 39, 59-65.
19. Jahic, M., Rotticci-Mulder, J. C., Martinelle, M., Hult, K., Enfors, S.-O., Modeling of growth and energy metabolism of Pichia pastoris producing a fusion protein. Bioprocess Biosyst. Eng. 2002, 24, 385-393.
20. Xie, J., Zhang, L., Ye, Q., Zhou, Q. et al., Angiostation production in cultivation of recombinant Pichia pastoris fed with mixed carbon sources. Biotechnol. Lett. 2003, 25, 173-177.
21. Cunha, A. E., Clemente, J. J., Gomes, R., Pinto, F. et al., Methanol induction optimization for scFv antibody fragment production in Pichia pastoris. Biotechnol. Bioeng. 2004, 86, 458-467.
22. Zhang, W., Bevins, M. A., Plantz, B. A., Smith, L. A., Meagher, M. M., Modeling Pichia pastoris growth on methanol and optimizing the production of a recombinant protein, the heavy-chain fragment C of Botulinum neurotoxin, Serotype A. Biotechnol. Bioeng. 2000, 70, 1-8.
23. Hohenblum, H., Borth, N., Mattanovich, D., Assessing viability and cell-associated product of recombinant protein producing Pichia pastoris with flow cytometry. J. Biotechnol. 2003, 102, 281-290.
24. Hohenblum, H., Gasser, B., Maurer, M., Borth, N., Mattanovich, D., Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris. Biotechnol. Bioeng. 2004, 85, 367-375.
25. Kauffman, K. J., Pridgen, E. M., Doyle, F. J. I., Dhurjati, P. S., Robinson, A. S., Decreased protein expression and intermittent recoveries in BiP levels result from cellular stress during heterologous protein expression in Saccharomyces cerevisiae. Biotechnol. Prog. 2002, 18, 942-950.
26. Sun, R., Anderson, T. J., Erickson, A. K., Nelson, E. A., Francis, D. H., Inhibition of adhesion of Escherichia coli k88ac fimbria to its receptor, intestinal mucin-type glycoproteins, by a monoclonal antibody directed against a variable domain of the fimbria. Infect. Immun. 2000, 68, 3509-3515.
27. Rautio, J., Barken, K. B., Lahdenpera, J., Breitenstein, A., Molin, S., Neubauer, P., Sandwich hybridisation assay for quantitative detection of yeast RNAs in crude cell lysates. Microb. Cell Fact. 2003, 2, 4.
28. Resina, D., Bollok, M., Khatri, N. K., Valero, F., Neubauer, P., Ferrer, P., Transcriptional response of P. pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induction. Microb. Cell Fact. 2007, 6, 21.
29. Ruottinen, M., Bollok, M., Kogler, M., Neubauer, A. et al., Improved production of human type II procollagen in the yeast Pichia pastoris in shake flasks by a wireless-controlled fed-batch system. BMC. Biotechnol. 2008, 8, 33.
30. Khatri, N. K., Hoffmann, F., Oxygen-limited control of methanol uptake for improved production of a single-chain antibody fragment with recombinant Pichia pastoris. Appl. Microbiol. Biotechnol. 2006, 72, 492-498.
31. Khatri, N. K., Hoffmann, F., Impact of methanol concentration on secreted protein production in oxygen-limited cultures of recombinant Pichia pastoris. Biotechnol. Bioeng. 2006, 93, 871-879.
32. Gasser, B., Sauer, M., Maurer, M., Stadlmayr, G., Mattanovich, D., Transcriptomics-based identification of novel factors enhancing heterologous protein secretion in yeasts. Appl. Environ. Microbiol. 2007, 73, 6499-6507.
33. Brierley, R. A., Siegel, R. S., Bussineau, C. M., Craig, W. S., et al., Mixed feed recombinant yeast fermentation. In The Patent Cooperation Treaty 1990, C12N, 5/00, C12 21/00, US.
34. Jahic, M., Veide, A., Charoenrat, T., Teeri, T., Enfors, S.-O., Process technology for production and recovery of heterologous proteins with Pichia pastoris. Biotechnol. Prog. 2006, 226, 6, 1465-1473.
35. Brinkmann, U., Mueller, R. H., Babel, W., The growth rate-limiting reaction in methanol-assimilating yeasts. FEMS Microbiol. Rev. 1990, 7, 261-265.