High-temperature cultivation of recombinant Pichia pastoris increases endoplasmic reticulum stress and decreases production of human interleukin-10

Microbial Cell Factories

Volumn 13, Issue 1, 2014, Pages 1-10

  Zhong, Yongjun ^a,b   Yang, Lu ^a,b   Guo, Yugang ^a,b   Fang, Fang ^a,b   Wang, Dong ^a,b   Li, Rui ^a,b   Jiang, Ming ^a,b   Kang, Wenyao ^a,b   Ma, Jiajia ^a,b   Sun, Jie ^a,b   Xiao, Weihua ^a,b  

^a UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA   (China)

^b UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA   (China)

Author keywords

ER stress; ER phagy; High temperature cultivation; Misfolded protein; Pichia pastoris; Unfolded protein response

Indexed keywords

CALCIUM ION; FUNGAL RNA; REACTIVE OXYGEN METABOLITE; RECOMBINANT HUMAN INTERLEUKIN 10; RECOMBINANT INTERLEUKIN 10; SIGNAL PEPTIDE; UNCLASSIFIED DRUG; IL10 PROTEIN, HUMAN; INTERLEUKIN 10; RECOMBINANT PROTEIN;

ARTICLE; AUTOPHAGOSOME; AUTOPHAGY; CELL DEATH; CELL MATURATION; CONTROLLED STUDY; CYTOKINE PRODUCTION; ENDOPLASMIC RETICULUM ASSOCIATED DEGRADATION; ENDOPLASMIC RETICULUM STRESS; ENZYME LINKED IMMUNOSORBENT ASSAY; FED BATCH FERMENTATION; FUNGAL CELL CULTURE; FUNGAL STRAIN; GLYCOSYLATION; HIGH TEMPERATURE CULTIVATION; HIGH TEMPERATURE PROCEDURES; HUMAN; KOMAGATAELLA PASTORIS; NONHUMAN; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN GLYCOSYLATION; PROTEIN PROCESSING; RETICULOPHAGY; RNA EXTRACTION; TRANSMISSION ELECTRON MICROSCOPY; UNFOLDED PROTEIN RESPONSE; WESTERN BLOTTING; YEAST CELL; BIOSYNTHESIS; GENETICS; HEAT; METABOLISM; PICHIA;

PICHIA PASTORIS;

ENDOPLASMIC RETICULUM STRESS; HOT TEMPERATURE; HUMANS; INTERLEUKIN-10; PICHIA; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; UNFOLDED PROTEIN RESPONSE;

EID: 84925500419     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-014-0163-7     Document Type: Article

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