The effect of α-mating factor secretion signal mutations on recombinant protein expression in Pichia pastoris

Gene

Volumn 519, Issue 2, 2013, Pages 311-317

  Lin Cereghino, Geoff P ^a   Stark, Carolyn M ^a   Kim, Daniel ^a   Chang, Jennifer ^a   Shaheen, Nadia ^a   Poerwanto, Hansel ^a   Agari, Kimiko ^a   Moua, Pachai ^a   Low, Lauren K ^a   Tran, Namphuong ^a   Huang, Amy D ^a   Nattestad, Maria ^a   Oshiro, Kristin T ^a   Chang, John William ^a   Chavan, Archana ^a   Tsai, Jerry W ^a   Lin Cereghino, Joan ^a  

^a UNIVERSITY OF THE PACIFIC   (United States)

Author keywords

Mating factor secretion signal; Pichia pastoris; Recombinant protein expression

Indexed keywords

AMINO ACID; HORSERADISH PEROXIDASE; RECOMBINANT PROTEIN;

ARTICLE; GENE DELETION; GENE MUTATION; MUTAGENESIS; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE;

AMINO ACID SEQUENCE; BLOTTING, WESTERN; GENE DELETION; GENE EXPRESSION REGULATION, FUNGAL; GENES, REPORTER; HORSERADISH PEROXIDASE; LIPASE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PEPTIDES; PICHIA; PLASMIDS; REAL-TIME POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE;

ARMORACIA RUSTICANA; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 84875579379     PISSN: 03781119     EISSN: 18790038     Source Type: Journal    
DOI: 10.1016/j.gene.2013.01.062     Document Type: Article

References (33)

1. Bieszke J.A., Spudich E.N., Scott K.L., Borkovich K.A., Spudich J.L. A eukaryotic protein, NOP-1, binds retinal to form an archaeal rhodopsin-like photochemically reactive pigment. Biochemistry 1999, 38:14138-14145.
2. Brake A.J., et al. Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. U. S. A. 1984, 81:4642-4646.
3. Cereghino J.L., Cregg J.M. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 2000, 24:45-66.
4. Chaudhuri B., Steube K., Stephan C. The pro-region of the yeast prepro-alpha-factor is essential for membrane translocation of human insulin-like growth factor 1 in vivo. Eur. J. Biochem. 1992, 206:793-800.
5. Cole C., Barber J.D., Barton G.J. The Jpred 3 secondary structure prediction server. Nucleic Acids Res. 2008, 36:W197-W201.
6. Fabre E., Nicaud J.M., Lopez M.C., Gaillardin C. Role of the proregion in the production and secretion of the Yarrowia lipolytica alkaline extracellular protease. J. Biol. Chem. 1991, 266:3782-3790.
7. Hegde R.S., Bernstein H.D. The surprising complexity of signal sequences. Trends Biochem. Sci. 2006, 31:563-571.
8. Joo H., Chavan A.G., Phan J., Day R., Tsai J. An amino acid packing code for α-helical structure and protein design. J. Mol. Biol. 2012, 419:234-254.
9. Kaiser C.A., Preuss D., Grisafi P., Botstein D. Many random sequences functionally replace the secretion signal sequence of yeast invertase. Science 1987, 235:312-317.
10. Kjeldsen T., et al. A removable spacer peptide in an alpha-factor-leader/insulin precursor fusion protein improves processing and concomitant yield of the insulin precursor in Saccharomyces cerevisiae. Gene 1996, 170:107-112.
11. Kjeldsen T., et al. Synthetic leaders with potential BiP binding mediate high-yield secretion of correctly folded insulin precursors from Saccharomyces cerevisiae. Protein Expr. Purif. 1997, 9:331-336.
12. Krainer F.W., Dietzsch C., Hajek T., Herwig C., Spadiut O., Glieder A. Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway. Microb. Cell Fact. 2012, 11:22.
13. Kurjan J., Herskowitz I. Structure of a yeast pheromone gene (MF alpha): a putative alpha-factor precursor contains four tandem copies of mature alpha-factor. Cell 1982, 30:933-943.
14. Li Z., et al. Secretion and proteolysis of heterologous proteins fused to the Escherichia coli maltose binding protein in Pichia pastoris. Protein Expr. Purif. 2010, 72:113-124.
15. Lin-Cereghino G.P., et al. Mxr1p, a key regulator of the methanol utilization pathway and peroxisomal genes in Pichia pastoris. Mol. Cell. Biol. 2006, 26:883-897.
16. Lin-Cereghino G.P., Leung W., Lin-Cereghino J. Expression of protein in Pichia pastoris. Expression Systems: Methods Express 2007, 123-145. Scion Publishing Limited, Oxfordshire. M. Dyson, Y. Durocher (Eds.).
17. Lin-Cereghino J., et al. Direct selection of Pichia pastoris expression strains using new G418 resistance vectors. Yeast 2008, 25:293-299.
18. Lin-Cereghino J., et al. Condensed protocol for competent cell preparation and transformation of the methylotrophic yeast Pichia pastoris. Biotechniques 2005, 38:44-48.
19. Macauley-Patrick S., Fazenda M.L., McNeil B., Harvey L.M. Heterologous protein production using the Pichia pastoris expression system. Yeast 2005, 22:249-270.
20. Morawski B., Lin Z., Cirino P., Joo H., Bandara G., Arnold F.H. Functional expression of horseradish peroxidase in Saccharomyces cerevisiae and Pichia pastoris. Protein Eng. 2000, 13:377-384.
21. Muller P.Y., Janovjak H., Miserez A.R., Dobbie Z. Processing of gene expression data generated by quantitative real-time RT-PCR. Biotechniques 2002, 32:1372-1374. (1376, 1378-9).
22. Pettersen E.F., et al. UCSF Chimera-a visualization system for exploratory research and analysis. J. Comput. Chem. 2004, 25:1605-1612.
23. Raemaekers R.J., de Muro L., Gatehouse J.A., Fordham-Skelton A.P. Functional phytohemagglutinin (PHA) and Galanthus nivalis agglutinin (GNA) expressed in Pichia pastoris correct N-terminal processing and secretion of heterologous proteins expressed using the PHA-E signal peptide. Eur. J. Biochem. 1999, 265:394-403.
24. Rakestraw J.A., Sazinsky S.L., Piatesi A., Antipov E., Wittrup K.D. Directed evolution of a secretory leader for the improved expression of heterologous proteins and full-length antibodies in Saccharomyces cerevisiae. Biotechnol. Bioeng. 2009, 103:1192-1201.
25. Sali A., Blundell T.L. Comparative protein modelling by satisfaction of spatial restraints. J. Mol. Biol. 1993, 234:779-815.
26. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Handbook 1989, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. 2nd ed.
27. Smith R.A., Duncan M.J., Moir D.T. Heterologous protein secretion from yeast. Science 1985, 229:1219-1224.
28. Stern B., Olsen L., Trosse C., Ravneberg H., Pryme I. Improving mammalian cell factories: the selection of signal peptide has major impact on recombinant protein synthesis and secretion in mammalian cells. Trends Cell Mol. Biol. 2007, 2:1-17.
29. Thor D., et al. Cloning and characterization of the Pichia pastoris MET2 gene as a selectable marker. FEMS Yeast Res. 2005, 5:935-942.
30. Verripsab T., Duboc P., Visser C., Sagt C. From gene to product in yeast: production of fungal cutinase. Enzyme Microb. Technol. 2000, 26:812-818.
31. Weis R., Luiten R., Skranc W., Schwab H., Wubbolts M., Glieder A. Reliable high-throughput screening with Pichia pastoris by limiting yeast cell death phenomena. FEMS Yeast Res. 2004, 2:179-189.
32. Xiong A.S., Yao Q.H., Peng R.H., Han P.L., Cheng Z.M., Li Y. High level expression of a recombinant acid phytase gene in Pichia pastoris. J. Appl. Microbiol. 2005, 98:418-428.
33. Zhang N., Suen W.C., Windsor W., Xiao L., Madison V., Zaks A. Improving tolerance of Candida antarctica lipase B towards irreversible thermal inactivation through directed evolution. Protein Eng. 2003, 16:599-605.