Production of complex human glycoproteins in yeast

Science

Volumn 301, Issue 5637, 2003, Pages 1244-1246

  Hamilton, Stephen R ^a,b   Bobrowicz, Piotr ^a,b   Bobrowicz, Beata ^a,b   Davidson, Robert C ^a,b   Li, Huijuan ^a,b   Mitchell, Teresa ^a,b   Nett, Juergen H ^a,b   Rausch, Sebastian ^a,b   Stadheim, Terrance A ^a,b   Wischnewski, Harry ^a,b   Wildt, Stefan ^a,b   Gerngross, Tillman U ^a  

^a DARTMOUTH COLLEGE   (United States)

^b GlycoFi Incorporated   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MOLECULAR STRUCTURE; SUGAR (SUCROSE); YEAST;

GLYCOSYLATION;

ENZYMES;

GLYCAN; GLYCOPROTEIN; MANNOSIDASE; N ACETYLGLUCOSAMINE; N ACETYLGLUCOSAMINYLTRANSFERASE; URIDINE DIPHOSPHATE;

PROTEIN; YEAST;

ARTICLE; ENZYME LOCALIZATION; GLYCOPROTEIN SYNTHESIS; GLYCOSYLATION; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN LOCALIZATION; STRUCTURE ACTIVITY RELATION; YEAST;

ANIMALS; CATALYTIC DOMAIN; ENDOPLASMIC RETICULUM; GENETIC ENGINEERING; GLYCOPROTEINS; GLYCOSYLATION; GOLGI APPARATUS; HUMANS; MANNOSIDASES; MEMBRANE TRANSPORT PROTEINS; N-ACETYLGLUCOSAMINYLTRANSFERASES; PEPTIDE LIBRARY; PICHIA; POLYSACCHARIDES; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; TRANSFORMATION, GENETIC;

EUKARYOTA; FUNGI; PICHIA; PICHIA PASTORIS;

EID: 0042322600     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1088166     Document Type: Article

References (9)

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9. We thank A. Prasad and N. Nutile-McMenemy for technical assistance and acknowledge the support of National Institute of Standards and Technology, Advance Technology Program grant 70NANB2H3046, and NIH Small Business Innovative Research grant 1R43GM66690-1.