Directed gene copy number amplification in Pichia pastoris by vector integration into the ribosomal DNA locus

FEMS Yeast Research

Volumn 9, Issue 8, 2009, Pages 1260-1270

  Marx, Hans ^a   Mecklenbräuker, Astrid ^a   Gasser, Brigitte ^a   Sauer, Michael ^a,b   Mattanovich, Diethard ^a,b  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b Fachhochschule Campus Wien   (Austria)

Author keywords

Heterologous protein expression; Human serum albumin; Human superoxide dismutase; Pichia pastoris; Post transformational vector amplification; RDNA integration

Indexed keywords

RIBOSOME DNA; SUPEROXIDE DISMUTASE;

ARTICLE; CONTROLLED STUDY; CYTOPLASM; DNA INTEGRATION; FUNGAL STRAIN; GENE AMPLIFICATION; GENE LOCUS; NONHUMAN; PICHIA PASTORIS; PROTEIN EXPRESSION;

DNA, FUNGAL; DNA, RIBOSOMAL; GENE DOSAGE; GENES, FUNGAL; GENETICS, MICROBIAL; HUMANS; PICHIA; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; SERUM ALBUMIN; SUPEROXIDE DISMUTASE;

PICHIA PASTORIS;

EID: 70350538686     PISSN: 15671356     EISSN: 15671364     Source Type: Journal    
DOI: 10.1111/j.1567-1364.2009.00561.x     Document Type: Article

References (33)

1. Chen H, Yen C, Tsai T et al 2006) Production and characterization of human extracellular superoxide dismutase in the methylotrophic yeast Pichia pastoris. J Agr Food Chem 54 : 8041 8047.
2. Clare J, Romanos M, Rayment F et al 1991) Production of mouse epidermal growth factor in yeast: high-level secretion using Pichia pastoris strains containing multiple gene copies. Gene 105 : 205 212.
3. Cox H, Mead D, Sudbery P, Eland R, Mannazzu I Evans L (2000) Constitutive expression of recombinant proteins in the methylotrophic yeast Hansenula polymorpha using the PMA1 promoter. Yeast 16 : 1191 1203.
4. Domínguez A, Fermiñán E, Sánchez M et al 1998) Non-conventional yeasts as hosts for heterologous protein production. Int Microbiol 1 : 131 142.
5. Fleer R, Yeh P, Amellal N et al 1991) Stable multicopy vectors for high-level secretion of recombinant human serum albumin by Kluyveromyces yeasts. Biotechnology 9 : 968 975.
6. Fridovich I (1975) Superoxide dismutases. Annu Rev Biochem 44 : 147 159.
7. Gasser B, Sauer M, Maurer M, Stadlmayr G Mattanovich D (2007) Transcriptomics-based identification of novel factors enhancing heterologous protein secretion in yeasts. Appl Environ Microb 73 : 6499 6507.
8. Gasser B, Mattanovich D, Sauer M Stadlmayr G (2008a) Expression system. International Patent Application WO2008128701.
9. Gasser B, Saloheimo M, Rinas U et al 2008b) Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview. Microb Cell Fact 7 : 11.
10. Gonzalez R, Andrews B, Molitor J Asenjo J (2003) Metabolic analysis of the synthesis of high levels of intracellular human SOD in Saccharomyces cerevisiae rhSOD 2060 411 SGA122. Biotechnol Bioeng 82 : 152 169.
11. Hartman J, Geller T, Yavin Z, Bartfeld D, Kanner D, Aviv H Gorecki M (1986) High-level expression of enzymatically active human Cu/Zn superoxide dismutase in Escherichia coli. P Natl Acad Sci USA 83 : 7142 7146.
12. Hartner F, Ruth C, Langenegger D et al 2008) Promoter library designed for fine-tuned gene expression in Pichia pastoris. Nucleic Acids Res 36 : e76.
13. Hohenblum H, Gasser B, Maurer M, Borth N Mattanovich D (2004) Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris. Biotechnol Bioeng 85 : 367 375.
14. Inan M, Aryasomayajula D, Sinha J Meagher M (2006) Enhancement of protein secretion in Pichia pastoris by overexpression of protein disulfide isomerase. Biotechnol Bioeng 93 : 771 778.
15. Inan M, Fanders S, Zhang W, Hotez P, Zhan B Meagher M (2007) Saturation of the secretory pathway by overexpression of a hookworm (Necator americanus) protein (Na-ASP1). Methods Mol Biol 389 : 65 76.
16. Kang H, Kang W, Hong W et al 2001) Development of expression systems for the production of recombinant human serum albumin using the MOX promoter in Hansenula polymorpha DL-1. Biotechnol Bioeng 76 : 175 185.
17. Klabunde J, Diesel A, Waschk D, Gellissen G, Hollenberg C Suckow M (2002) Single-step co-integration of multiple expressible heterologous genes into the ribosomal DNA of the methylotrophic yeast Hansenula polymorpha. Appl Microbiol Biot 58 : 797 805.
18. Kobayashi K, Kuwae S, Ohya T et al 2000) High-level expression of recombinant human serum albumin from the methylotrophic yeast Pichia pastoris with minimal protease production and activation. J Biosci Bioeng 89 : 55 61. (Pubitemid 30099950)
19. Kühleitner M, Gasser B Mattanovich D (2006) Versatile modeling and optimization of fed batch processes for the production of secreted heterologous proteins with Pichia pastoris. Microb Cell Fact 5 : 37.
20. Lopes T, Klootwijk J, Veenstra A, van der Aar P, van Heerikhuizen H, Raúe H Planta R (1989) High-copy-number integration into the ribosomal DNA of Saccharomyces cerevisiae: a new vector for high-level expression. Gene 79 : 199 206.
21. Lopes T, Hakkaart G, Koerts B, Raué H Planta R (1991) Mechanism of high-copy-number integration of pMIRY-type vectors into the ribosomal DNA of Saccharomyces cerevisiae. Gene 105 : 83 90.
22. Macauley-Patrick S, Fazenda M, McNeil B Harvey L (2005) Heterologous protein production using the Pichia pastoris expression system. Yeast 22 : 249 270.
23. Mattanovich D, Weik R, Thim S, Kramer W, Bayer K Katinger H (1996) Optimization of recombinant gene expression in Escherichia coli. Ann NY Acad Sci 782 : 182 190.
24. Minghetti P, Ruffner D, Kuang W, Dennison O, Hawkins J, Beattie W Dugaiczyk A (1986) Molecular structure of the human albumin gene is revealed by nucleotide sequence within q11-22 of chromosome 4. J Biol Chem 261 : 6747 6757.
25. Porstmann T, Wietschke R, Schmechta H et al 1988) A rapid and sensitive enzyme immunoassay for Cu/Zn superoxide dismutase with polyclonal and monoclonal antibodies. Clin Chim Acta 171 : 1 10.
26. Resina D, Michael M, Oriol C et al 2009) Engineering of bottlenecks in Rhizopus oryzae lipase production in Pichia pastoris using the nitrogen source-regulated FLD1 promoter. New Biotechnology, DOI
27. Romanos M (1995) Advances in the use of Pichia pastoris for high-level gene expression. Curr Opin Biotech 6 : 527 533.
28. Rösel H Kunze G (1998) Integrative transformation of the dimorphic yeast Arxula adeninivorans LS3 based on hygromycin B resistance. Curr Genet 33 : 157 163.
29. Sreekrishna K, Brankamp R, Kropp K et al 1997) Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris. Gene 190 : 55 62.
30. Steinborn G, Gellissen G Kunze G (2005) Assessment of Hansenula polymorpha and Arxula adeninivorans-derived rDNA-targeting elements for the design of Arxula adeninivorans expression vectors. FEMS Yeast Res 5 : 1047 1054.
31. Steinborn G, Böer E, Scholz A, Tag K, Kunze G Gellissen G (2006) Application of a wide-range yeast vector (CoMed) system to recombinant protein production in dimorphic Arxula adeninivorans, methylotrophic Hansenula polymorpha and other yeasts. Microb Cell Fact 5 : 33.
32. Sunga A, Tolstorukov I Cregg J (2008) Posttransformational vector amplification in the yeast Pichia pastoris. FEMS Yeast Res 8 : 870 876.
33. Wartmann T, Rösel H, Kunze I, Bode R Kunze G (1998) AILV1 gene from the yeast Arxula adeninivorans LS3-a new selective transformation marker. Yeast 14 : 1017 1025.