Cooverexpression of chaperones for enhanced secretion of a single-chain antibody fragment in Pichia pastoris

Applied Microbiology and Biotechnology

Volumn 74, Issue 2, 2007, Pages 381-389

  Damasceno, Leonardo M ^a   Anderson, Kyle A ^a   Ritter, Gerd ^b   Cregg, James M ^c   Old, Lloyd J ^b   Batt, Carl A ^a  

^a Department of Obstetrics Gynecology   (United States)

^b MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^c KECK GRADUATE INSTITUTE   (United States)

Author keywords

A33; Chaperone; Pichia pastoris; Recombinant protein; scFv

Indexed keywords

ANTIBODIES; FERMENTATION; METHANOL; PROTEINS;

A33; CHAPERONE; PICHIA PASTORIS; RECOMBINANT PROTEIN; SCFV;

ENZYME KINETICS;

CARBON; CHAPERONE; FUNGAL PROTEIN; GLUCOSE REGULATED PROTEIN 78; METHANOL; PROTEIN DISULFIDE ISOMERASE; RECOMBINANT PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY; GENE EXPRESSION; GENETIC ENGINEERING; PROTEIN; SECRETION; YEAST;

ANTIBODY PRODUCTION; ARTICLE; CARBON SOURCE; CONTROLLED STUDY; DISULFIDE BOND; ENDOPLASMIC RETICULUM; ENERGY RESOURCE; FUNGAL STRAIN; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; PROTEIN EXPRESSION; PROTEIN FOLDING;

AMINO ACID SEQUENCE; BIOTECHNOLOGY; GENETIC ENGINEERING; HEAT-SHOCK PROTEINS; IMMUNOGLOBULIN FRAGMENTS; MOLECULAR CHAPERONES; MOLECULAR SEQUENCE DATA; PICHIA; PROTEIN DISULFIDE-ISOMERASE; RECOMBINANT PROTEINS;

PICHIA PASTORIS;

EID: 33846841181     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-006-0652-7     Document Type: Article

References (45)

1. Bernales S, Papa FR, Walter P (2006) Intracellular signaling by the unfolded protein response. Annu Rev Cell Dev Biol (in press)
2. Brodsky JL, Werner ED, Dubas ME, Goeckeler JL, Kruse KB, McCracken AA (1999) The requirement for molecular chaperones during endoplasmic reticulum-associated protein degradation demonstrates that protein export and import are mechanistically distinct. J Biol Chem 274(6):3453-3460
3. Cereghino GP, Cereghino JL, Ilgen C, Cregg JM (2002) Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr Opin Biotechnol 13(4):329-332
4. Cregg JM, Vedvick TS, Raschke WC (1993) Recent advances in the expression of foreign genes in Pichia pastoris. Biotechnology (N Y) 11(8):905-910
5. Damasceno LM, Pla I, Chang HJ, Cohen L, Ritter G, Old LJ, Batt CA (2004) An optimized fermentation process for high-level production of a single-chain Fv antibody fragment in Pichia pastoris. Protein Expr Purif 37(1):18-26
6. Ellgaard L, Helenius A (2003) Quality control in the endoplasmic reticulum. Nat Rev Mol Cell Biol 4(3):181-191
7. Haas IG, Wabl M (1983) Immunoglobulin heavy chain binding protein. Nature 306(5941):387-389
8. Hammond C, Helenius A (1994) Quality control in the secretory pathway: retention of a misfolded viral membrane glycoprotein involves cycling between the ER, intermediate compartment, and Golgi apparatus. J Cell Biol 126(1):41-52
9. Harmsen MM, Bruyne MI, Raue HA, Maat J (1996) Overexpression of binding protein and disruption of the PMR1 gene synergistically stimulate secretion of bovine prochymosin but not plant thaumatin in yeast. Appl Microbiol Biotechnol 46(4):365-370
10. Horton P, Nakai K (1997) Better prediction of protein cellular localization sites with the k nearest neighbors classifier. Proc Int Conf Intell Syst Mol Biol 5:147-152
11. Hsu TA, Eiden JJ, Bourgarel P, Meo T, Betenbaugh MJ (1994) Effects of co-expressing chaperone BiP on functional antibody production in the baculovirus system. Protein Expr Purif 5(6):595-603
12. Inan M, Aryasomayajula D, Sinha J, Meagher MM (2005) Enhancement of protein secretion in Pichia pastoris by overexpression of protein disulfide isomerase. Biotechnol Bioeng 93(4):771-778
13. Klein C, de Lamotte-Guery F, Gautier F, Moulin G, Boze H, Joudrier P, Gautier MF (1998) High-level secretion of a wheat lipid transfer protein in Pichia pastoris. Protein Expr Purif 13(1):73-82
14. Knappik A, Pluckthun A (1995) Engineered turns of a recombinant antibody improve its in vivo folding. Protein Eng 8(1):81-89
15. Kobayashi K, Kuwae S, Ohya T, Ohda T, Ohyama M, Tomomitsu K (2000) High level secretion of recombinant human serum albumin by fed-batch fermentation of the methylotrophic yeast, Pichia pastoris, based on optimal methanol feeding strategy. J Biosci Bioeng 90(3):280-288
16. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
17. Liu YY, Woo JH, Neville DM (2003) Targeted introduction of a diphtheria toxin resistant mutation into the chromosomal EF-2 locus of Pichia pastoris and expression of immunotoxin in the EF-2 mutants. Protein Expr Purif 30(2):262-274
18. Liu YY, Woo JH, Neville DM Jr (2005) Overexpression of an anti-CD3 immunotoxin increases expression and secretion of molecular chaperone BiP/Kar2p by Pichia pastoris. Appl Environ Microbiol 71(9):5332-5340
19. Lodish HF, Kong N, Snider M, Strous GJ (1983) Hepatoma secretory proteins migrate from rough endoplasmic reticulum to Golgi at characteristic rates. Nature 304(5921):80-83
20. Lyles MM, Gilbert HF (1991) Catalysis of the oxidative folding of ribonuclease A by protein disulfide isomerase: dependence of the rate on the composition of the redox buffer. Biochemistry 30(3):613-619
21. Macauley-Patrick S, Fazenda ML, McNeil B, Harvey LM (2005) Heterologous protein production using the Pichia pastoris expression system. Yeast 22(4):249-270
22. Matlack KE, Misselwitz B, Plath K, Rapoport TA (1999) BiP acts as a molecular ratchet during posttranslational transport of preproalpha factor across the ER membrane. Cell 97(5):553-564
23. Mattanovich D, Gasser B, Hohenblum H, Sauer M (2004) Stress in recombinant protein producing yeasts. J Biotechnol 113(1-3):121-135
24. Nicholson RC, Williams DB, Moran LA (1990) An essential member of the HSP70 gene family of Saccharomyces cerevisiae is homologous to immunoglobulin heavy chain binding protein. Proc Natl Acad Sci U S A 87(3):1159-1163
25. Nieba L, Honegger A, Krebber C, Pluckthun A (1997) Disrupting the hydrophobic patches at the antibody variable/constant domain interface: improved in vivo folding and physical characterization of an engineered scFv fragment. Protein Eng 10(4):435-444
26. Ohba H, Harano T, Omura T (1981) Intracellular and intramembranous localization of a protein disulfide isomerase in rat liver. J Biochem (Tokyo) 89(3):889-900
27. Parekh R, Forrester K, Wittrup D (1995) Multicopy overexpression of bovine pancreatic trypsin inhibitor saturates the protein folding and secretory capacity of Saccharomyces cerevisiae. Protein Expr Purif 6(4):537-545
28. Primm TP, Walker KW, Gilbert HF (1996) Facilitated protein aggregation. Effects of calcium on the chaperone and anti-chaperone activity of protein disulfide-isomerase. J Biol Chem 271(52):33664-33669
29. Prinz B, Stahl U, Lang C (2003) Intracellular transport of a heterologous membrane protein, the human transferrin receptor, in Saccharomyces cerevisiae. Int Microbiol 6(1):49-55
30. Puig A, Gilbert HF (1994) Protein disulfide isomerase exhibits chaperone and anti-chaperone activity in the oxidative refolding of lysozyme. J Biol Chem 269(10):7764-7771
31. Robinson AS, Hines V, Wittrup KD (1994) Protein disulfide isomerase overexpression increases secretion of foreign proteins in Saccharomyces cerevisiae. Biotechnology (N Y) 12(4):381-384
32. Rowling PJ, Freedman RB (1993) Folding, assembly, and posttranslational modification of proteins within the lumen of the endoplasmic reticulum. Subcell Biochem 21:41-80
33. Sambrook J, Fritsch EF, Maniatis T (1989)Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
34. Schroder M, Kaufman RJ (2005) ER stress and the unfolded protein response. Mutat Res 569(1-2):29-63
35. Shusta EV, Raines RT, Pluckthun A, Wittrup KD (1998) Increasing the secretory capacity of Saccharomyces cerevisiae for production of single-chain antibody fragments. Nat Biotechnol 16(8):773-777
36. Shuster JR (1991) Gene expression in yeast: protein secretion. Curr Opin Biotechnol 2(5):685-690
37. Smith JD, Robinson AS (2002) Overexpression of an archaeal protein in yeast: secretion bottleneck at the ER. Biotechnol Bioeng 79(7):713-723
38. Smith JD, Tang BC, Robinson AS (2004) Protein disulfide isomerase, but not binding protein, overexpression enhances secretion of a non-disulfide-bonded protein in yeast. Biotechnol Bioeng 85(3):340-350
39. Song JL, Wang CC (1995) Chaperone-like activity of protein disulfide-isomerase in the refolding of rhodanese. Eur J Biochem 231(2):312-316
40. Tsai B, Ye Y, Rapoport TA (2002) Retro-translocation of proteins from the endoplasmic reticulum into the cytosol. Nat Rev Mol Cell Biol 3(4):246-255
41. Vad R, Nafstad E, Dahl LA, Gabrielsen OS (2005) Engineering of a Pichia pastoris expression system for secretion of high amounts of intact human parathyroid hormone. J Biotechnol 116(3):251-260
42. van der Heide M, Hollenberg CP, van der Klei IJ, Veenhuis M (2002) Overproduction of BiP negatively affects the secretion of Aspergillus niger glucose oxidase by the yeast Hansenula polymorpha. Appl Microbiol Biotechnol 58(4):487-494
43. van Vliet C, Thomas EC, Merino-Trigo A, Teasdale RD, Gleeson PA (2003) Intracellular sorting and transport of proteins. Prog Biophys Mol Biol 83(1):1-45
44. Warsame A, Vad R, Kristensen T, Oyen TB (2001) Characterization of a gene encoding a Pichia pastoris protein disulfide isomerase. Biochem Biophys Res Commun 281(5):1176-1182
45. Wilkinson B, Gilbert HF (2004) Protein disulfide isomerase. Biochim Biophys Acta 1699(1-2):35-44