O-linked glycosylation analysis of recombinant human granulocyte colony-stimulating factor produced in glycoengineered Pichia pastoris by liquid chromatography and mass spectrometry

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 945-946, Issue , 2014, Pages 135-140

  Gong, Bing ^a   Burnina, Irina ^a   Lynaugh, Heather ^a   Li, Huijuan ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

HPAEC PAD; LC MS; O linked mannosylation; RhG CSF

Indexed keywords

ESTERIFICATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PRODUCTION PLATFORMS; PROTEINS; QUALITY CONTROL; YEAST;

GLYCOENGINEERED PICHIA PASTORIS; GRANULOCYTE-COLONY STIMULATING FACTOR; HPAEC-PAD; LC-MS; O-LINKED; PRODUCT QUALITY CONTROL; RHG-CSF; TANDEM MASS SPECTROMETRY TECHNIQUES;

GLYCOSYLATION;

CYANOGEN BROMIDE; FORMIC ACID; GLYCAN; PROTEINASE; RECOMBINANT GRANULOCYTE COLONY STIMULATING FACTOR;

ARTICLE; GLYCOSYLATION; LIQUID CHROMATOGRAPHY; PEPTIDE MAPPING; PICHIA PASTORIS; PRIORITY JOURNAL; TANDEM MASS SPECTROMETRY; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY;

PICHIA; PICHIA PASTORIS;

HPAEC-PAD; LC-MS; O-LINKED MANNOSYLATION; RHG-CSF;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; GENETIC ENGINEERING; GLYCOPROTEINS; GLYCOSYLATION; GRANULOCYTE COLONY-STIMULATING FACTOR; HUMANS; MANNOSE; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PICHIA; RECOMBINANT PROTEINS;

EID: 84890378301     PISSN: 15700232     EISSN: 1873376X     Source Type: Journal    
DOI: 10.1016/j.jchromb.2013.11.031     Document Type: Article

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