-
2
-
-
0003458664
-
-
Wiley-VCH Verlag GmbH, Weinheim
-
Drauz, K., and Waldmann, H. eds (2002) Enzyme Catalysis in Organic Synthesis,2nd edn, Vol. 1 -3, Wiley-VCH Verlag GmbH, Weinheim .
-
(2002)
Enzyme Catalysis in Organic Synthesis,2nd edn
, vol.1-3
-
-
Drauz, K.1
Waldmann, H.2
-
4
-
-
85055477441
-
Biocatalysis in the Pharmaceutical, and Biotechnological Industries,1st edn
-
CRC Press/Taylor & Francis Group, New York
-
Patel, R.N. ed. (2006) Biocatalysis in the Pharmaceutical, and Biotechnological Industries,1st edn, CRC Press/Taylor & Francis Group, New York .
-
(2006)
-
-
Patel, R.N.1
-
5
-
-
0035313593
-
Improved biocatalysts by directed evolution, and rational protein design
-
Bornscheuer, U.T., and Pohl, M. (2001) Improved biocatalysts by directed evolution, and rational protein design . Current Opinion in Chemical Biology,5,137-43 .
-
(2001)
Current Opinion in Chemical Biology
, vol.5
, pp. 137-43
-
-
Bornscheuer, U.T.1
Pohl, M.2
-
6
-
-
85045502002
-
Randomization of genes by PCR mutagenesis
-
Caldwell, R.C., and Joyce, G.F. (1992) Randomization of genes by PCR mutagenesis . PCR Methods, and Applications,2,28-33 .
-
(1992)
PCR Methods, and Applications
, vol.2
, pp. 28-33
-
-
Caldwell, R.C.1
Joyce, G.F.2
-
7
-
-
0002694056
-
A method for random mutagenesis of a defi ned DNA segment using a modifi ed polymerase chain reaction
-
Leung, D.W., Chen, E., and Goeddel, D.V. (1989) A method for random mutagenesis of a defi ned DNA segment using a modifi ed polymerase chain reaction . Technique,1,11-15 .
-
(1989)
Technique
, vol.1
, pp. 11-15
-
-
Leung, D.W.1
Chen, E.2
Goeddel, D.V.3
-
8
-
-
20444443943
-
Multiplex-PCR-based recombination as a novel high-fi delity method for directed evolution
-
Eggert, T., Funke, S.A., Rao, N.M., Acharya, P., Krumm, H., Reetz, M.T., and Jaeger, K.E. (2005) Multiplex-PCR-based recombination as a novel high-fi delity method for directed evolution . ChemBioChem,6,1062-7 .
-
(2005)
ChemBioChem
, vol.6
, pp. 1062-7
-
-
Eggert, T.1
Funke, S.A.2
Rao, N.M.3
Acharya, P.4
Krumm, H.5
Reetz, M.T.6
Jaeger, K.E.7
-
9
-
-
34248567845
-
Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes
-
Reetz, M.T., and Carballeira, J.D. (2007) Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes . Nature Biotechnology,2,891-903 .
-
(2007)
Nature Biotechnology
, vol.2
, pp. 891-903
-
-
Reetz, M.T.1
Carballeira, J.D.2
-
10
-
-
33845288649
-
Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability
-
Reetz, M.T., Carballeira, J.D., and Vogel, A. (2006) Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability . Angewandte Chemie-International Edition,45,7745-51 .
-
(2006)
Angewandte Chemie-International Edition
, vol.45
, pp. 7745-51
-
-
Reetz, M.T.1
Carballeira, J.D.2
Vogel, A.3
-
11
-
-
33744475011
-
Directed evolution of enantioselective enzymes: Iterative cycles of CASTing for probing protein-sequence space
-
erratum 249
-
Reetz, M.T., Wang, L.W., and Bocola, M. (2006) Directed evolution of enantioselective enzymes: Iterative cycles of CASTing for probing protein-sequence space . Angewandte Chemie-International Edition,45,1236-41 ; erratum 2494.
-
(2006)
Angewandte Chemie-International Edition
, vol.45
, pp. 1236-41
-
-
Reetz, M.T.1
Wang, L.W.2
Bocola, M.3
-
12
-
-
33947095042
-
Improving catalytic function by ProSAR-driven enzyme evolution
-
Fox, R.J., Davis, S.C., Mundorff, E.C., Newman, L.M., Gavrilovic, V., Ma, S.K., Chung, L.M., Ching, C., Tam, S., Muley, S., Grate, J., Gruber, J., Whitman, J.C., Sheldon, R.A., and Huisman, G.W. (2007) Improving catalytic function by ProSAR-driven enzyme evolution . Nature Biotechnology,25,338-44 .
-
(2007)
Nature Biotechnology
, vol.25
, pp. 338-44
-
-
Fox, R.J.1
Davis, S.C.2
Mundorff, E.C.3
Newman, L.M.4
Gavrilovic, V.5
Ma, S.K.6
Chung, L.M.7
Ching, C.8
Tam, S.9
Muley, S.10
Grate, J.11
Gruber, J.12
Whitman, J.C.13
Sheldon, R.A.14
Huisman, G.W.15
-
13
-
-
33744804647
-
-
Wiley-VCH Verlag GmbH, Weinheim
-
Reymond, J.L. ed. (2005) Enzyme Assays, Wiley-VCH Verlag GmbH, Weinheim .
-
(2005)
Enzyme Assays
-
-
Reymond, J.L.1
-
14
-
-
11144306351
-
High-throughput-screening systems for hydrolases
-
Bornscheuer, U.T. (2004) High-throughput-screening systems for hydrolases . Engineering in Life Sciences,4,539-42 .
-
(2004)
Engineering in Life Sciences
, vol.4
, pp. 539-42
-
-
Bornscheuer, U.T.1
-
15
-
-
18044397530
-
High-throughput assays for lipases, and esterases
-
Schmidt, M., and Bornscheuer, U.T. (2005) High-throughput assays for lipases, and esterases . Biomolecular Engineering,22,51-6 .
-
(2005)
Biomolecular Engineering
, vol.22
, pp. 51-6
-
-
Schmidt, M.1
Bornscheuer, U.T.2
-
19
-
-
84885776721
-
Selection of phage-displayed enzymes, in Evolutionary Methods in Biotechnology
-
Wiley-VCH Verlag GmbH, Weinheim
-
Soumillon, P. (2004) Selection of phage-displayed enzymes, in Evolutionary Methods in Biotechnology. Clever Tricks for Directed Evolution (ed. A. Schwienhorst), Wiley-VCH Verlag GmbH, Weinheim, pp. 47-64 .
-
(2004)
Clever Tricks for Directed Evolution (ed. A. Schwienhorst)
, pp. 47-64
-
-
Soumillon, P.1
-
20
-
-
0037073004
-
Functional esterase surface display by the autotransporter pathway in Escherichia coli
-
Schultheiss, E., Paar, C., Schwab, H., and Jose, J. (2002) Functional esterase surface display by the autotransporter pathway in Escherichia coli . Journal of Molecular Catalysis B, Enzymatic,18,89-97 .
-
(2002)
Journal of Molecular Catalysis B, Enzymatic
, vol.18
, pp. 89-97
-
-
Schultheiss, E.1
Paar, C.2
Schwab, H.3
Jose, J.4
-
21
-
-
0033772183
-
Function-based isolation of novel enzymes from a large library
-
Olsen, M.J., Stephens, D., Griffiths, D., Daugherty, P., Georgiou, G., and Iverson, B.L. (2000) Function-based isolation of novel enzymes from a large library . Nature Biotechnology,18,1071-4 .
-
(2000)
Nature Biotechnology
, vol.18
, pp. 1071-4
-
-
Olsen, M.J.1
Stephens, D.2
Griffiths, D.3
Daugherty, P.4
Georgiou, G.5
Iverson, B.L.6
-
22
-
-
3543063987
-
Ultra-high-throughput screening based on cell-surface display, and fl uorescence-activated cell sorting for the identifi cation of novel biocatalysts
-
Becker, S., Schmoldt, H.U., Adams, T.M., Wilhelm, S., and Kolmar, H. (2004) Ultra-high-throughput screening based on cell-surface display, and fl uorescence-activated cell sorting for the identifi cation of novel biocatalysts . Current Opinion in Biotechnology,15,323-9 .
-
(2004)
Current Opinion in Biotechnology
, vol.15
, pp. 323-9
-
-
Becker, S.1
Schmoldt, H.U.2
Adams, T.M.3
Wilhelm, S.4
Kolmar, H.5
-
23
-
-
34447526318
-
Ultrahigh-throughput screening to identify E. coli cells expressing functionally active enzymes on their surface
-
Becker, S., Michalczyk, A., Wilhelm, S., Jaeger, K.E., and Kolmar, H. (2007) Ultrahigh-throughput screening to identify E. coli cells expressing functionally active enzymes on their surface . ChemBioChem,8,943-9 .
-
(2007)
ChemBioChem
, vol.8
, pp. 943-9
-
-
Becker, S.1
Michalczyk, A.2
Wilhelm, S.3
Jaeger, K.E.4
Kolmar, H.5
-
24
-
-
11144295610
-
Screening for novel industrial biocatalysts
-
Lorenz, P., and Eck, J. (2004) Screening for novel industrial biocatalysts . Engineering in Life Sciences,4,501-4 .
-
(2004)
Engineering in Life Sciences
, vol.4
, pp. 501-4
-
-
Lorenz, P.1
Eck, J.2
-
25
-
-
0036080680
-
A screening system for enantioselective enzymes based on differential cell growth
-
Reetz, M.T., and Rüggeberg, C.J. (2002) A screening system for enantioselective enzymes based on differential cell growth . Chemical Communications,1428-9 .
-
(2002)
Chemical Communications
, pp. 1428-9
-
-
Reetz, M.T.1
Rüggeberg, C.J.2
-
26
-
-
0034730195
-
Directed evolution of a (beta alpha)(8)-barrel enzyme to catalyze related reactions in two different metabolic pathways
-
Jürgens, C., Strom, A., Wegener, D., Hettwer, S., Wilmanns, M., and Sterner, R. (2000) Directed evolution of a (beta alpha)(8)-barrel enzyme to catalyze related reactions in two different metabolic pathways . Proceedings of the National Academy of Sciences of the United States of America,97,9925-30 .
-
(2000)
Proceedings of the National Academy of Sciences of the United States of America
, vol.97
, pp. 9925-30
-
-
Jürgens, C.1
Strom, A.2
Wegener, D.3
Hettwer, S.4
Wilmanns, M.5
Sterner, R.6
-
27
-
-
0032549781
-
Redesigning enzyme topology by directed evolution
-
MacBeath, G., Kast, P., and Hilvert, D. (1998) Redesigning enzyme topology by directed evolution . Science,279,1958-61 .
-
(1998)
Science
, vol.279
, pp. 1958-61
-
-
MacBeath, G.1
Kast, P.2
Hilvert, D.3
-
28
-
-
0032828603
-
Directed evolution of an esterase: Screening of enzyme libraries based on pH-indicators, and a growth assay
-
Bornscheuer, U.T., Altenbuchner, J., and Meyer, H.H. (1999) Directed evolution of an esterase: Screening of enzyme libraries based on pH-indicators, and a growth assay . Bioorganic, and Medicinal Chemistry,7,2169-73 .
-
(1999)
Bioorganic, and Medicinal Chemistry
, vol.7
, pp. 2169-73
-
-
Bornscheuer, U.T.1
Altenbuchner, J.2
Meyer, H.H.3
-
29
-
-
0032486517
-
Directed evolution of an esterase for the stereoselective resolution of a key intermediate in the synthesis of epothilones
-
Bornscheuer, U.T., Altenbuchner, J., and Meyer, H.H. (1998) Directed evolution of an esterase for the stereoselective resolution of a key intermediate in the synthesis of epothilones . Biotechnology, and Bioengineering,58,554-9 .
-
(1998)
Biotechnology, and Bioengineering
, vol.58
, pp. 554-9
-
-
Bornscheuer, U.T.1
Altenbuchner, J.2
Meyer, H.H.3
-
30
-
-
12244253039
-
A high-throughput, low-volume enzyme assay on solid support
-
Babiak, P., and Reymond, J.L. (2005) A high-throughput, low-volume enzyme assay on solid support . Analytical Chemistry,77,373-7 .
-
(2005)
Analytical Chemistry
, vol.77
, pp. 373-7
-
-
Babiak, P.1
Reymond, J.L.2
-
31
-
-
0032853779
-
Directed evolution of an esterase from Pseudomonas fl uorescens. Random mutagenesis by error-prone PCR or a mutator strain, and identifi cation of mutants showing enhanced enantioselectivity by a resorufi n-based fl uorescence assay
-
Henke, E., and Bornscheuer, U.T. (1999) Directed evolution of an esterase from Pseudomonas fl uorescens . Random mutagenesis by error-prone PCR or a mutator strain, and identifi cation of mutants showing enhanced enantioselectivity by a resorufi n-based fl uorescence assay . Biological Chemistry,380,1029-33 .
-
(1999)
Biological Chemistry
, vol.380
, pp. 1029-33
-
-
Henke, E.1
Bornscheuer, U.T.2
-
32
-
-
0344987533
-
Resorufi n acetate as substrate for determination of hydrolytic enzymes at low enzyme, and substrate concentrations
-
Kramer, D.N., and Guilbault, G.G. (1964) Resorufi n acetate as substrate for determination of hydrolytic enzymes at low enzyme, and substrate concentrations . Analytical Chemistry,36,1662-3 .
-
(1964)
Analytical Chemistry
, vol.36
, pp. 1662-3
-
-
Kramer, D.N.1
Guilbault, G.G.2
-
33
-
-
4644334092
-
Universal chromogenic substrates for lipases, and esterases
-
Grognux, J., Wahler, D., Nyfeler, E., and Reymond, J.L. (2004) Universal chromogenic substrates for lipases, and esterases . Tetrahedron: Asymmetry,15,2981-9 .
-
(2004)
Tetrahedron: Asymmetry
, vol.15
, pp. 2981-9
-
-
Grognux, J.1
Wahler, D.2
Nyfeler, E.3
Reymond, J.L.4
-
34
-
-
0000646394
-
Enantioselectivity of a recombinant esterase from Pseudomonas fl uorescens towards alcohols, and carboxylic acids
-
Krebsfänger, N., Schierholz, K., and Bornscheuer, U.T. (1998) Enantioselectivity of a recombinant esterase from Pseudomonas fl uorescens towards alcohols, and carboxylic acids . Journal of Biotechnology,60,105-11 .
-
(1998)
Journal of Biotechnology
, vol.60
, pp. 105-11
-
-
Krebsfänger, N.1
Schierholz, K.2
Bornscheuer, U.T.3
-
35
-
-
0017170678
-
Phospholipase-C assay using para-nitrophenylphosphorylcholine together with sorbitol, and its application to studying metal, and detergent requirement of enzyme
-
Kurioka, S., and Matsuda, M. (1976) Phospholipase-C assay using para-nitrophenylphosphorylcholine together with sorbitol, and its application to studying metal, and detergent requirement of enzyme . Analytical Biochemistry,75,281-9 .
-
(1976)
Analytical Biochemistry
, vol.75
, pp. 281-9
-
-
Kurioka, S.1
Matsuda, M.2
-
36
-
-
0028111533
-
Lipase of Pseudomonas cepacia for biotechnological purposes-purifi cation, crystallization, and characterization
-
Bornscheuer, U., Reif, O.W., Lausch, R., Freitag, R., Scheper, T., Kolisis, F.N., and Menge, U. (1994) Lipase of Pseudomonas cepacia for biotechnological purposes-purifi cation, crystallization, and characterization . Biochimica et Biophysica Acta,1201,55-60 .
-
(1994)
Biochimica et Biophysica Acta
, vol.1201
, pp. 55-60
-
-
Bornscheuer, U.1
Reif, O.W.2
Lausch, R.3
Freitag, R.4
Scheper, T.5
Kolisis, F.N.6
Menge, U.7
-
37
-
-
0028241550
-
Determination of lipase activity by a rhodamine-triglyceride-agarose assay
-
Jette, J.F., and Ziomek, E. (1994) Determination of lipase activity by a rhodamine-triglyceride-agarose assay . Analytical Biochemistry,219,256-60 .
-
(1994)
Analytical Biochemistry
, vol.219
, pp. 256-60
-
-
Jette, J.F.1
Ziomek, E.2
-
38
-
-
0023089142
-
Specifi c, and sensitive plate assay for bacterial lipases
-
Kouker, G., and Jaeger, K.E. (1987) Specifi c, and sensitive plate assay for bacterial lipases . Applied, and Environmental Microbiology,53,211-13 .
-
(1987)
Applied, and Environmental Microbiology
, vol.53
, pp. 211-13
-
-
Kouker, G.1
Jaeger, K.E.2
-
39
-
-
0033064950
-
Enantioselective fl uorogenic assay of acetate hydrolysis for detecting lipase catalytic antibodies
-
Klein, G., and Reymond, J.L. (1999) Enantioselective fl uorogenic assay of acetate hydrolysis for detecting lipase catalytic antibodies . Helvetica Chimica Acta,82,400-7 .
-
(1999)
Helvetica Chimica Acta
, vol.82
, pp. 400-7
-
-
Klein, G.1
Reymond, J.L.2
-
42
-
-
0037471193
-
A high-throughput-screening method for determining the synthetic activity of hydrolases
-
Konarzycka-Bessler, M., and Bornscheuer, U.T. (2003) A high-throughput-screening method for determining the synthetic activity of hydrolases . Angewandte Chemie-International Edition,42,1418-20 .
-
(2003)
Angewandte Chemie-International Edition
, vol.42
, pp. 1418-20
-
-
Konarzycka-Bessler, M.1
Bornscheuer, U.T.2
-
43
-
-
33744455764
-
A fi lter paper-based assay for laboratory evolution of hydrolases, and dehydrogenases
-
Wong, T.S., Schwaneberg, U., Stürmer, R., Hauer, B., and Breuer, M. (2006) A fi lter paper-based assay for laboratory evolution of hydrolases, and dehydrogenases . Combinatorial Chemistry, and High Throughput Screening,9,289-93 .
-
(2006)
Combinatorial Chemistry, and High Throughput Screening
, vol.9
, pp. 289-93
-
-
Wong, T.S.1
Schwaneberg, U.2
Stürmer, R.3
Hauer, B.4
Breuer, M.5
-
44
-
-
0035915157
-
A high-throughput-screening method for the identifi cation of active, and enantioselective hydrolases
-
Baumann, M., Stürmer, R., and Bornscheuer, U.T. (2001) A high-throughput-screening method for the identifi cation of active, and enantioselective hydrolases . Angewandte Chemie-International Edition,40,4201-4 .
-
(2001)
Angewandte Chemie-International Edition
, vol.40
, pp. 4201-4
-
-
Baumann, M.1
Stürmer, R.2
Bornscheuer, U.T.3
-
45
-
-
20644469267
-
Quantitative analyses of biochemical kinetic resolutions of enantiomers
-
Chen, C.S., Fujimoto, Y., Girdaukas, G., and Sih, C.J. (1982) Quantitative analyses of biochemical kinetic resolutions of enantiomers . Journal of the American Chemical Society,104,7294-9 .
-
(1982)
Journal of the American Chemical Society
, vol.104
, pp. 7294-9
-
-
Chen, C.S.1
Fujimoto, Y.2
Girdaukas, G.3
Sih, C.J.4
-
46
-
-
0032080371
-
An enantioselective fl uorimetric assay for alcohol dehydrogenases using albumin-catalyzed beta-elimination of umbelliferone
-
Klein, G., and Reymond, J.L. (1998) An enantioselective fl uorimetric assay for alcohol dehydrogenases using albumin-catalyzed beta-elimination of umbelliferone . Bioorganic, and Medicinal Chemistry Letters,8,1113-16 .
-
(1998)
Bioorganic, and Medicinal Chemistry Letters
, vol.8
, pp. 1113-16
-
-
Klein, G.1
Reymond, J.L.2
-
47
-
-
0037871909
-
A low background high-throughput screening (HTS) fl uorescence assay for lipases, and esterases using acyloxymethylethers of umbelliferone
-
Leroy, E., Bensel, N., and Reymond, J.L. (2003) A low background high-throughput screening (HTS) fl uorescence assay for lipases, and esterases using acyloxymethylethers of umbelliferone . Bioorganic, and Medicinal Chemistry Letters,13,2105-8 .
-
(2003)
Bioorganic, and Medicinal Chemistry Letters
, vol.13
, pp. 2105-8
-
-
Leroy, E.1
Bensel, N.2
Reymond, J.L.3
-
48
-
-
3543062056
-
Classifying enzymes from selectivity fi ngerprints
-
Grognux, J., and Reymond, J.L. (2004) Classifying enzymes from selectivity fi ngerprints . ChemBioChem,5,826-31 .
-
(2004)
ChemBioChem
, vol.5
, pp. 826-31
-
-
Grognux, J.1
Reymond, J.L.2
-
49
-
-
0346334537
-
Adrenaline profi ling of lipases, and esterases with 1,2-diol, and carbohydrate acetates
-
Wahler, D., Boujard, O., Lefevre, F., and Reymond, J.L. (2004) Adrenaline profi ling of lipases, and esterases with 1,2-diol, and carbohydrate acetates . Tetrahedron,60,703-10 .
-
(2004)
Tetrahedron
, vol.60
, pp. 703-10
-
-
Wahler, D.1
Boujard, O.2
Lefevre, F.3
Reymond, J.L.4
-
50
-
-
0342754143
-
Quick E. A fast spectroscopic method to measure the enantioselectivity of hydrolases
-
Janes, L.E., and Kazlauskas, R.J. (1997) Quick E. A fast spectroscopic method to measure the enantioselectivity of hydrolases . The Journal of Organic Chemistry,62,4560-1 .
-
(1997)
The Journal of Organic Chemistry
, vol.62
, pp. 4560-1
-
-
Janes, L.E.1
Kazlauskas, R.J.2
-
51
-
-
4644281805
-
Mapping the substrate selectivity, and enantioselectivity of esterases from thermophiles
-
Somers, N.A., and Kazlauskas, R.J. (2004) Mapping the substrate selectivity, and enantioselectivity of esterases from thermophiles . Tetrahedron Asymmetry,15,2991-3004 .
-
(2004)
Tetrahedron Asymmetry
, vol.15
, pp. 2991-3004
-
-
Somers, N.A.1
Kazlauskas, R.J.2
-
52
-
-
0035911359
-
Mapping the substrate selectivity of new hydrolases using colorimetric screening: lipases from Bacillus thermocatenulatus, and Phiostoma piliferum, esterases from Pseudomonas fl uorescens, and Streptomyces diastatochromogenes
-
Liu, A.M.F., Somers, N.A., Kazlauskas, R.J., Brush, T.S., Zocher, F., Enzelberger, M.M., Bornscheuer, U.T., Horsman, G.P., Mezzetti, A., Schmidt-Dannert, C., and Schmid, R.D. (2001) Mapping the substrate selectivity of new hydrolases using colorimetric screening: lipases from Bacillus thermocatenulatus, and Phiostoma piliferum, esterases from Pseudomonas fl uorescens, and Streptomyces diastatochromogenes . Tetrahedron Asymmetry,12,545-56 .
-
(2001)
Tetrahedron Asymmetry
, vol.12
, pp. 545-56
-
-
Liu, A.M.F.1
Somers, N.A.2
Kazlauskas, R.J.3
Brush, T.S.4
Zocher, F.5
Enzelberger, M.M.6
Bornscheuer, U.T.7
Horsman, G.P.8
Mezzetti, A.9
Schmidt-Dannert, C.10
Schmid, R.D.11
-
53
-
-
0038814072
-
Mutations in distant residues moderately increase the enantioselectivity of Pseudomonas fl uorescens esterase toward methyl 3-bromo-2-methylpropanoate, and ethyl 3-phenylbutyrate
-
Horsman, G.P., Liu, A.M.F., Henke, E., Bornscheuer, U.T., and Kazlauskas, R.J. (2003) Mutations in distant residues moderately increase the enantioselectivity of Pseudomonas fl uorescens esterase toward methyl 3-bromo-2-methylpropanoate, and ethyl 3-phenylbutyrate . Chemistry-A European Journal,9,1933-9 .
-
(2003)
Chemistry-A European Journal
, vol.9
, pp. 1933-9
-
-
Horsman, G.P.1
Liu, A.M.F.2
Henke, E.3
Bornscheuer, U.T.4
Kazlauskas, R.J.5
-
54
-
-
0035875094
-
A GC-based method for high-throughput screening of enantioselective catalysts
-
Reetz, M.T., Kühling, K.M., Wilensek, S., Husmann, H., Häusig, U.W., and Hermes, M. (2001) A GC-based method for high-throughput screening of enantioselective catalysts . Catalysis Today,67,389-96 .
-
(2001)
Catalysis Today
, vol.67
, pp. 389-96
-
-
Reetz, M.T.1
Kühling, K.M.2
Wilensek, S.3
Husmann, H.4
Häusig, U.W.5
Hermes, M.6
-
55
-
-
0033553835
-
A method for high-throughput screening of enantioselective catalysts
-
Reetz, M.T., Becker, M.H., Klein, H.W., and Stöckigt, D. (1999) A method for high-throughput screening of enantioselective catalysts . Angewandte Chemie-International Edition,38,1758-61 .
-
(1999)
Angewandte Chemie-International Edition
, vol.38
, pp. 1758-61
-
-
Reetz, M.T.1
Becker, M.H.2
Klein, H.W.3
Stöckigt, D.4
-
56
-
-
0034602059
-
Super-high-throughput screening of enantioselective catalysts by using capillary array electrophoresis
-
Reetz, M.T., Kühling, K.M., Deege, A., Hinrichs, H., and Belder, D. (2000) Super-high-throughput screening of enantioselective catalysts by using capillary array electrophoresis . Angewandte Chemie-International Edition,39,3891-3 .
-
(2000)
Angewandte Chemie-International Edition
, vol.39
, pp. 3891-3
-
-
Reetz, M.T.1
Kühling, K.M.2
Deege, A.3
Hinrichs, H.4
Belder, D.5
-
57
-
-
18144383856
-
pHluorin-based in vivo assay for hydrolase screening
-
Schuster, S., Enzelberger, M., Trauthwein, H., Schmid, R.D., and Urlacher, V.B. (2005) pHluorin-based in vivo assay for hydrolase screening . Analytical Biochemistry,77,2727-32 .
-
(2005)
Analytical Biochemistry
, vol.77
, pp. 2727-32
-
-
Schuster, S.1
Enzelberger, M.2
Trauthwein, H.3
Schmid, R.D.4
Urlacher, V.B.5
-
58
-
-
3042647616
-
Anchored periplasmic expression, a versatile technology for the isolation of high-affi nity antibodies from Escherichia coli-expressed libraries
-
Harvey, B.R., Georgiou, G., Hayhurst, A., Jeong, K.J., and Iverson, B.L. (2004) Anchored periplasmic expression, a versatile technology for the isolation of high-affi nity antibodies from Escherichia coli-expressed libraries . Proceedings of the National Academy of Sciences of the United States of America,101,9193-8 .
-
(2004)
Proceedings of the National Academy of Sciences of the United States of America
, vol.101
, pp. 9193-8
-
-
Harvey, B.R.1
Georgiou, G.2
Hayhurst, A.3
Jeong, K.J.4
Iverson, B.L.5
-
60
-
-
0035424822
-
Protein engineering by cell-surface display
-
Wittrup, K.D. (2001) Protein engineering by cell-surface display . Current Opinion in Biotechnology,12,395-9 .
-
(2001)
Current Opinion in Biotechnology
, vol.12
, pp. 395-9
-
-
Wittrup, K.D.1
-
61
-
-
0037008439
-
Heptyl oleate synthesis as useful tool to discriminate between lipases, proteases, and other hydrolases in crude preparations
-
de Maria, P.D., Martinez-Alzamora, F., Moreno, S.P., Valero, F., Rua, M.L., Sanchez-Montero, J.M., Sinisterra, J.V., and Alcantara, A.R. (2002) Heptyl oleate synthesis as useful tool to discriminate between lipases, proteases, and other hydrolases in crude preparations . Enzyme, and Microbial Technology,31,283-8 .
-
(2002)
Enzyme, and Microbial Technology
, vol.31
, pp. 283-8
-
-
de Maria, P.D.1
Martinez-Alzamora, F.2
Moreno, S.P.3
Valero, F.4
Rua, M.L.5
Sanchez-Montero, J.M.6
Sinisterra, J.V.7
Alcantara, A.R.8
-
62
-
-
0029201412
-
Simple screening method for lipase for transesterifi cation in organic solvent
-
Furutani, T., Su, R.H., Ooshima, H., and Kato, J. (1995) Simple screening method for lipase for transesterifi cation in organic solvent . Enzyme, and Microbial Technology,17,1067-72 .
-
(1995)
Enzyme, and Microbial Technology
, vol.17
, pp. 1067-72
-
-
Furutani, T.1
Su, R.H.2
Ooshima, H.3
Kato, J.4
-
63
-
-
0033751721
-
An esterifi cation method for determination of lipase activity
-
Kiran, K.R., Krishna, S.H., Babu, C.V.S., Karanth, N.G., and Divakar, S. (2000) An esterifi cation method for determination of lipase activity . Biotechnology Letters,22,1511-14 .
-
(2000)
Biotechnology Letters
, vol.22
, pp. 1511-14
-
-
Kiran, K.R.1
Krishna, S.H.2
Babu, C.V.S.3
Karanth, N.G.4
Divakar, S.5
-
64
-
-
33846189983
-
Immobilization of enzymes in microtiter plate scale
-
Brandt, B., Hidalgo, A., and Bornscheuer, U.T. (2006) Immobilization of enzymes in microtiter plate scale . Biotechnology Journal,1,582-7 .
-
(2006)
Biotechnology Journal
, vol.1
, pp. 582-7
-
-
Brandt, B.1
Hidalgo, A.2
Bornscheuer, U.T.3
-
65
-
-
33947327091
-
A modifi ed para-nitrophenyl palmitate assay for lipase synthetic activity determination in organic solvent
-
Teng, Y., and Xu, Y. (2007) A modifi ed para-nitrophenyl palmitate assay for lipase synthetic activity determination in organic solvent . Analytical Biochemistry,363,297-9 .
-
(2007)
Analytical Biochemistry
, vol.363
, pp. 297-9
-
-
Teng, Y.1
Xu, Y.2
-
66
-
-
0029670577
-
Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents
-
Moore, J.C., and Arnold, F.H. (1996) Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents . Nature Biotechnology,14,458-67 .
-
(1996)
Nature Biotechnology
, vol.14
, pp. 458-67
-
-
Moore, J.C.1
Arnold, F.H.2
-
67
-
-
0032573217
-
Directed evolution of a thermostable esterase
-
Giver, L., Gershenson, A., Freskgard, P.O., and Arnold, F.H. (1998) Directed evolution of a thermostable esterase . Proceedings of the National Academy of Sciences of the United States of America,95,12809-13 .
-
(1998)
Proceedings of the National Academy of Sciences of the United States of America
, vol.95
, pp. 12809-13
-
-
Giver, L.1
Gershenson, A.2
Freskgard, P.O.3
Arnold, F.H.4
-
68
-
-
0038236305
-
A molecular mechanism of enantiorecognition of tertiary alcohols by carboxylesterases
-
Henke, E., Bornscheuer, U.T., Schmid, R.D., and Pleiss, J. (2003) A molecular mechanism of enantiorecognition of tertiary alcohols by carboxylesterases . ChemBioChem,4,485-93 .
-
(2003)
ChemBioChem
, vol.4
, pp. 485-93
-
-
Henke, E.1
Bornscheuer, U.T.2
Schmid, R.D.3
Pleiss, J.4
-
69
-
-
34047217953
-
Highly enantioselective kinetic resolution of two tertiary alcohols using mutants of an esterase from Bacillus subtilis
-
Heinze, B., Kourist, R., Fransson, L., Hult, K., and Bornscheuer, U.T. (2007) Highly enantioselective kinetic resolution of two tertiary alcohols using mutants of an esterase from Bacillus subtilis . Protein Engineering, Design, and Selection,20,125-31 .
-
(2007)
Protein Engineering, Design, and Selection
, vol.20
, pp. 125-31
-
-
Heinze, B.1
Kourist, R.2
Fransson, L.3
Hult, K.4
Bornscheuer, U.T.5
-
70
-
-
34547142296
-
Highly enantioselective synthesis of arylaliphatic tertiary alcohols using mutants of an esterase from Bacillus subtilis
-
Kourist, R., Bartsch, S., and Bornscheuer, U.T. (2007) Highly enantioselective synthesis of arylaliphatic tertiary alcohols using mutants of an esterase from Bacillus subtilis . Advanced Synthesis, and Catalysis,349,1393-8 .
-
(2007)
Advanced Synthesis, and Catalysis
, vol.349
, pp. 1393-8
-
-
Kourist, R.1
Bartsch, S.2
Bornscheuer, U.T.3
-
71
-
-
39849090377
-
Complete inversion of enantioselectivity towards acetylated tertiary alcohols by a double mutant of a Bacillus subtilis esterase
-
Bartsch, S., Kourist, R., and Bornscheuer, U.T. (2008) Complete inversion of enantioselectivity towards acetylated tertiary alcohols by a double mutant of a Bacillus subtilis esterase . Angewandte Chemie-International Edition,47,1508-11 .
-
(2008)
Angewandte Chemie-International Edition
, vol.47
, pp. 1508-11
-
-
Bartsch, S.1
Kourist, R.2
Bornscheuer, U.T.3
-
72
-
-
0033834791
-
Directed evolution of an enantioselective lipase
-
Liebeton, K., Zonta, A., Schimossek, K., Nardini, M., Lang, D., Dijkstra, B.W., Reetz, M.T., and Jaeger, K.E. (2000) Directed evolution of an enantioselective lipase . Chemistry, and Biology,7,709-18 .
-
(2000)
Chemistry, and Biology
, vol.7
, pp. 709-18
-
-
Liebeton, K.1
Zonta, A.2
Schimossek, K.3
Nardini, M.4
Lang, D.5
Dijkstra, B.W.6
Reetz, M.T.7
Jaeger, K.E.8
-
73
-
-
0035477024
-
Directed evolution of an enantioselective enzyme through combinatorial multiple-cassette mutagenesis
-
Reetz, M.T., Wilensek, S., Zha, D., and Jaeger, K.E. (2001) Directed evolution of an enantioselective enzyme through combinatorial multiple-cassette mutagenesis . Angewandte Chemie-International Edition,40,3589-91 .
-
(2001)
Angewandte Chemie-International Edition
, vol.40
, pp. 3589-91
-
-
Reetz, M.T.1
Wilensek, S.2
Zha, D.3
Jaeger, K.E.4
-
74
-
-
33846302851
-
Learning from directed evolution: further lessons from theoretical investigations into cooperative mutations in lipase enantioselectivity
-
Reetz, M.T., Puls, M., Carballeira, J.D., Vogel, A., Jaeger, K.E., Eggert, T., Thiel, W., Bocola, M., and Otte, N. (2007) Learning from directed evolution: further lessons from theoretical investigations into cooperative mutations in lipase enantioselectivity . ChemBioChem,8,106-12 .
-
(2007)
ChemBioChem
, vol.8
, pp. 106-12
-
-
Reetz, M.T.1
Puls, M.2
Carballeira, J.D.3
Vogel, A.4
Jaeger, K.E.5
Eggert, T.6
Thiel, W.7
Bocola, M.8
Otte, N.9
-
75
-
-
33646810869
-
Directed evolution of an esterase from Pseudomonas fl uorescens yields a mutant with excellent enantioselectivity, and activity for the kinetic resolution of a chiral building block
-
Schmidt, M., Hasenpusch, D., Kähler, M., Kirchner, U., Wiggenhorn, K., Langel, W., and Bornscheuer, U.T. (2006) Directed evolution of an esterase from Pseudomonas fl uorescens yields a mutant with excellent enantioselectivity, and activity for the kinetic resolution of a chiral building block . ChemBioChem,7,805-9 .
-
(2006)
ChemBioChem
, vol.7
, pp. 805-9
-
-
Schmidt, M.1
Hasenpusch, D.2
Kähler, M.3
Kirchner, U.4
Wiggenhorn, K.5
Langel, W.6
Bornscheuer, U.T.7
-
76
-
-
12344337569
-
Focusing mutations into the P. fl uorescens esterase binding site increases enantioselectivity more effectively than distant mutations
-
Park, S., Morley, K.L., Horsman, G.P., Holmquist, M., Hult, K., and Kazlauskas, R.J. (2005) Focusing mutations into the P. fl uorescens esterase binding site increases enantioselectivity more effectively than distant mutations . Chemistry, and Biology,12,45-54 .
-
(2005)
Chemistry, and Biology
, vol.12
, pp. 45-54
-
-
Park, S.1
Morley, K.L.2
Horsman, G.P.3
Holmquist, M.4
Hult, K.5
Kazlauskas, R.J.6
-
77
-
-
0031795317
-
One biocatalyst-many applications: The use of Candida antarctica B-lipase in organic synthesis
-
Anderson, E.M., Larsson, K.M., and Kirk, O. (1998) One biocatalyst-many applications: The use of Candida antarctica B-lipase in organic synthesis . Biocatalysis, and Biotransformation,16,181-204 .
-
(1998)
Biocatalysis, and Biotransformation
, vol.16
, pp. 181-204
-
-
Anderson, E.M.1
Larsson, K.M.2
Kirk, O.3
-
78
-
-
0141817121
-
Improving tolerance of Candida antarctica lipase B towards irreversible thermal inactivation through directed evolution
-
Zhang, N., Suen, W.C., Windsor, W., Xiao, L., Madison, V., and Zaks, A. (2003) Improving tolerance of Candida antarctica lipase B towards irreversible thermal inactivation through directed evolution . Protein Engineering,16,599-605 .
-
(2003)
Protein Engineering
, vol.16
, pp. 599-605
-
-
Zhang, N.1
Suen, W.C.2
Windsor, W.3
Xiao, L.4
Madison, V.5
Zaks, A.6
-
79
-
-
1842765645
-
Improved activity, and thermostability of Candida antarctica lipase B by DNA family shuffl ing
-
Suen, W.C., Zhang, N., Xiao, L., Madison, V., and Zaks, A. (2004) Improved activity, and thermostability of Candida antarctica lipase B by DNA family shuffl ing . Protein Engineering, Design, and Selection,17,133-40 .
-
(2004)
Protein Engineering, Design, and Selection
, vol.17
, pp. 133-40
-
-
Suen, W.C.1
Zhang, N.2
Xiao, L.3
Madison, V.4
Zaks, A.5
-
80
-
-
0042665295
-
Inverting enantioselectivity of Burkholderia cepacia KWI-56 Lipase by combinatorial mutation, and high-throughput screening using single-molecule PCR, and in vitro expression
-
Koga, Y., Kato, K., Nakano, H., and Yamane, T. (2003) Inverting enantioselectivity of Burkholderia cepacia KWI-56 Lipase by combinatorial mutation, and high-throughput screening using single-molecule PCR, and in vitro expression . Journal of Molecular Biology,331,585-92 .
-
(2003)
Journal of Molecular Biology
, vol.331
, pp. 585-92
-
-
Koga, Y.1
Kato, K.2
Nakano, H.3
Yamane, T.4
-
81
-
-
25844525807
-
Improving the catalytic activity of Candida antarctica lipase B by circular permutation
-
Qian, Z., and Lutz, S. (2005) Improving the catalytic activity of Candida antarctica lipase B by circular permutation . Journal of the American Chemical Society,127,13466-7 .
-
(2005)
Journal of the American Chemical Society
, vol.127
, pp. 13466-7
-
-
Qian, Z.1
Lutz, S.2
-
82
-
-
37549058473
-
Investigating the structural, and functional consequences of circular permutation on lipase B from Candida antarctica
-
Qian, Z., Fields, C.J., and Lutz, S. (2007) Investigating the structural, and functional consequences of circular permutation on lipase B from Candida antarctica . ChemBioChem,8,1989-96 .
-
(2007)
ChemBioChem
, vol.8
, pp. 1989-96
-
-
Qian, Z.1
Fields, C.J.2
Lutz, S.3
-
83
-
-
0028362197
-
Alteration of chain length selectivity of a Rhizopus delemar lipase through site-directed mutagenesis
-
Joerger, R.D., and Haas, M.J. (1994) Alteration of chain length selectivity of a Rhizopus delemar lipase through site-directed mutagenesis . Lipids,29,377-84 .
-
(1994)
Lipids
, vol.29
, pp. 377-84
-
-
Joerger, R.D.1
Haas, M.J.2
-
84
-
-
0031044124
-
Altered acyl chain length specifi city of Rhizopus delemar lipase through mutagenesis, and molecular modeling
-
Klein, R.R., King, G., Moreau, R.A., and Haas, M.J. (1997) Altered acyl chain length specifi city of Rhizopus delemar lipase through mutagenesis, and molecular modeling . Lipids,32,123-30 .
-
(1997)
Lipids
, vol.32
, pp. 123-30
-
-
Klein, R.R.1
King, G.2
Moreau, R.A.3
Haas, M.J.4
-
85
-
-
0035844981
-
In vitro selection of enzymatically active lipase variants from phage libraries using a mechanism-based inhibitor
-
Danielsen, S., Eklund, M., Deussen, H.J., Graslund, T., Nygren, P.A., and Borchert, T.V. (2001) In vitro selection of enzymatically active lipase variants from phage libraries using a mechanism-based inhibitor . Gene,272,267-74 .
-
(2001)
Gene
, vol.272
, pp. 267-74
-
-
Danielsen, S.1
Eklund, M.2
Deussen, H.J.3
Graslund, T.4
Nygren, P.A.5
Borchert, T.V.6
-
86
-
-
33645776206
-
Remote interactions explain the unusual regioselectivity of lipase from Pseudomonas cepacia toward the secondary hydroxyl of 2 '-deoxynucleosides
-
Lavandera, I., Fernandez, S., Magdalena, J., Ferrero, M., Grewal, H., Savile, C.K., Kazlauskas, R.J., and Gotor, V. (2006) Remote interactions explain the unusual regioselectivity of lipase from Pseudomonas cepacia toward the secondary hydroxyl of 2 '-deoxynucleosides . ChemBioChem,7,693-8 .
-
(2006)
ChemBioChem
, vol.7
, pp. 693-8
-
-
Lavandera, I.1
Fernandez, S.2
Magdalena, J.3
Ferrero, M.4
Grewal, H.5
Savile, C.K.6
Kazlauskas, R.J.7
Gotor, V.8
-
87
-
-
10044248344
-
Catalytic promiscuity in biocatalysis: using old enzymes to form new bonds, and follow new pathways
-
Bornscheuer, U.T., and Kazlauskas, R.J. (2004) Catalytic promiscuity in biocatalysis: using old enzymes to form new bonds, and follow new pathways . Angewandte Chemie-International Edition,43,6032-40 .
-
(2004)
Angewandte Chemie-International Edition
, vol.43
, pp. 6032-40
-
-
Bornscheuer, U.T.1
Kazlauskas, R.J.2
-
88
-
-
11244281311
-
The " evolvability " of promiscuous protein functions
-
Aharoni, A., Gaidukov, L., Khersonsky, O., McQ Gould, S., Roodveldt, C., and Tawfik, D.S. (2004) The " evolvability " of promiscuous protein functions . Nature Genetics,37,73-6 .
-
(2004)
Nature Genetics
, vol.37
, pp. 73-6
-
-
Aharoni, A.1
Gaidukov, L.2
Khersonsky, O.3
McQ Gould, S.4
Roodveldt, C.5
Tawfik, D.S.6
-
89
-
-
34247131307
-
Enzyme promiscuity: mechanism, and applications
-
Hult, K., and Berglund, P. (2007) Enzyme promiscuity: mechanism, and applications . Trends in Biotechnology,25,231-8 .
-
(2007)
Trends in Biotechnology
, vol.25
, pp. 231-8
-
-
Hult, K.1
Berglund, P.2
-
90
-
-
17644394187
-
Directed evolution of Pseudomonas aeruginosa lipase for improved amide-hydrolyzing activity
-
Fujii, R., Nakagawa, Y., Hiratake, J., Sogabe, A., and Sakata, K. (2005) Directed evolution of Pseudomonas aeruginosa lipase for improved amide-hydrolyzing activity . Protein Engineering, Design, and Selection,18,93-101 .
-
(2005)
Protein Engineering, Design, and Selection
, vol.18
, pp. 93-101
-
-
Fujii, R.1
Nakagawa, Y.2
Hiratake, J.3
Sogabe, A.4
Sakata, K.5
-
91
-
-
1242338176
-
Enhanced thermostability, and tolerance of high substrate concentration of an esterase by directed evolution
-
Kim, J.H., Choi, G.S., Kim, S.B., Kim, W.H., Lee, J.Y., Ryu, Y.W., and Kim, G.J. (2004) Enhanced thermostability, and tolerance of high substrate concentration of an esterase by directed evolution . Journal of Molecular Catalysis B, Enzymatic,27,169-75 .
-
(2004)
Journal of Molecular Catalysis B, Enzymatic
, vol.27
, pp. 169-75
-
-
Kim, J.H.1
Choi, G.S.2
Kim, S.B.3
Kim, W.H.4
Lee, J.Y.5
Ryu, Y.W.6
Kim, G.J.7
-
92
-
-
22144485602
-
Expanding the range of substrate acceptance of enzymes: combinatorial active-site saturation test
-
Reetz, M.T., Bocola, M., Carballeira, J.D., Zha, D., and Vogel, A. (2005) Expanding the range of substrate acceptance of enzymes: combinatorial active-site saturation test . Angewandte Chemie-International Edition,44,4192-6 .
-
(2005)
Angewandte Chemie-International Edition
, vol.44
, pp. 4192-6
-
-
Reetz, M.T.1
Bocola, M.2
Carballeira, J.D.3
Zha, D.4
Vogel, A.5
-
93
-
-
0035496145
-
Improved enantioselectivity of a lipase by rational protein engineering
-
Rotticci, D., Rotticci-Mulder, J.C., Denman, S., Norin, T., and Hult, K. (2001) Improved enantioselectivity of a lipase by rational protein engineering . ChemBioChem,2,766-70 .
-
(2001)
ChemBioChem
, vol.2
, pp. 766-70
-
-
Rotticci, D.1
Rotticci-Mulder, J.C.2
Denman, S.3
Norin, T.4
Hult, K.5
-
94
-
-
33947155728
-
Inverting enantioselectivity of Burkholderia gladioli esterase EstB by directed, and designed evolution
-
Ivancic, M., Valinger, G., Gruber, K., and Schwab, H. (2007) Inverting enantioselectivity of Burkholderia gladioli esterase EstB by directed, and designed evolution . Journal of Biotechnology,129,109-22 .
-
(2007)
Journal of Biotechnology
, vol.129
, pp. 109-22
-
-
Ivancic, M.1
Valinger, G.2
Gruber, K.3
Schwab, H.4
-
95
-
-
33947131105
-
Stability, and activity improvement of cephalosporin esterase EstB from Burkholderia gladioli by directed evolution, and structural interpretation of muteins
-
Valinger, G., Hermann, M., Wagner, U.G., and Schwab, H. (2007) Stability, and activity improvement of cephalosporin esterase EstB from Burkholderia gladioli by directed evolution, and structural interpretation of muteins . Journal of Biotechnology,129,98-108 .
-
(2007)
Journal of Biotechnology
, vol.129
, pp. 98-108
-
-
Valinger, G.1
Hermann, M.2
Wagner, U.G.3
Schwab, H.4
-
96
-
-
30444439246
-
Directed evolution of Bacillus subtilis lipase A by use of enantiomeric phosphonate inhibitors: Crystal structures, and phage display selection
-
Dröge, M.J., Boersma, Y.L., van Pouderoyen, G., Vrenken, T.E., Rüggeberg, C.J., Reetz, M.T., Dijkstra, B. W., and Quax, W.J. (2006) Directed evolution of Bacillus subtilis lipase A by use of enantiomeric phosphonate inhibitors: Crystal structures, and phage display selection . ChemBioChem,7,149-57 .
-
(2006)
ChemBioChem
, vol.7
, pp. 149-57
-
-
Dröge, M.J.1
Boersma, Y.L.2
van Pouderoyen, G.3
Vrenken, T.E.4
Rüggeberg, C.J.5
Reetz, M.T.6
Dijkstra, B.W.7
Quax, W.J.8
-
97
-
-
33750608896
-
Improved thermostability, and the optimum temperature of Rhizopus arrhizus lipase by directed evolution
-
Niu, W.N., Li, Z.P., Zhang, D.W., Yu, M.R., and Tan, T.W. (2006) Improved thermostability, and the optimum temperature of Rhizopus arrhizus lipase by directed evolution . Journal of Molecular Catalysis B, Enzymatic,43,33-9 .
-
(2006)
Journal of Molecular Catalysis B, Enzymatic
, vol.43
, pp. 33-9
-
-
Niu, W.N.1
Li, Z.P.2
Zhang, D.W.3
Yu, M.R.4
Tan, T.W.5
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