Altered acyl chain length specificity of Rhizopus delemar lipase through mutagenesis and molecular modeling

Lipids

Volumn 32, Issue 2, 1997, Pages 123-130

  Klein, Robert R ^a   King, Gregory ^a   Moreau, Robert A ^a   Haas, Michael J ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; ASPARTIC ACID; FATTY ACID; GLUTAMINE; LEUCINE; LYSINE; PHENYLALANINE; SERINE; TRIACYLGLYCEROL; TRIACYLGLYCEROL LIPASE; TRIOCTANOIN; TRIOLEIN; TRYPTOPHAN; VALINE;

ACYLATION; AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME SPECIFICITY; ENZYME SUBSTRATE; MOLECULAR DYNAMICS; MOLECULAR MODEL; NONHUMAN; RHIZOPUS; SITE DIRECTED MUTAGENESIS;

LIPASE; MODELS, STRUCTURAL; MUTAGENESIS, SITE-DIRECTED; OCTANOIC ACIDS; RHIZOPUS; SUBSTRATE SPECIFICITY; TRIGLYCERIDES;

EID: 0031044124     PISSN: 00244201     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11745-997-0016-1     Document Type: Article

References (27)

1. Sarda, L., and Desnuelle, P. (1958) Action of Pancreatic Lipase on Esters in Emulsion, Biochim. Biophys. Acta 30, 513-521.
2. Derewenda, Z.S., and Sharp, A.M. (1993) News from the Interface: The Molecular Structures of Triacylglyceride Lipases, TIBS 18, 20-25.
3. Derewenda, Z.S. (1995) A Twist in the Tale of Lipolytic Enzymes, Structural Biology 2, 347-349.
4. Kazlauskas, R.J. (1994) Elucidating Structure-Mechanism Relationships in Lipases: Prospects for Predicting and Engineering Catalytic Properties, TIBTECH 12, 464-472.
5. Cygler, M., Grochulski, P., and Schrag, J.D. (1995) Structural Determinants Defining Common Stereoselectivity of Lipases Toward Secondary Alcohols, Can. J. Microbiol. 41, 289-296.
6. Stadler, P., Kovac, A., and Paltauf, F. (1995) Understanding Lipase Action and Selectivity, Croatica Chemica Acta 68, 649-674.
7. Quinlan, P., and Moore, S. (1993) Modification of Triglycerides by Lipases: Process Technology and its Application to the Production of Nutritionally Improved Fats, INFORM 4, 580-585.
8. Vulfson, E.N. (1994) Industrial Applications of Lipases, in Lipases, Their Structure, Biochemistry, and Application (Woolley, P., and Petersen, S.B., eds.) pp. 271-288, Cambridge University Press, Cambridge.
9. Haas, M.J., Cichowicz, D.J., and Bailey, D.G. (1992) Purification and Characterization of an Extracellular Lipase from the Fungus Rhizopus delemar, Lipids 27, 571-576.
10. Haas, M.J., Allen, J., and Berka, T.R. (1991) Cloning, Expression and Characterization of a cDNA Encoding a Lipase from Rhizopus delemar, Gene 109, 107-113.
11. Joerger, R.D., and Haas, M.J. (1993) Overexpression of a Rhizopus delemar Lipase Gene in Escherichia coli, Lipids 28, 81-87.
12. Derewenda, U., Swenson, L., Wei, Y., Green, R., Kobos, P.M., Joerger, R., Haas, M.J., and Derewenda, Z.S. (1994) Conformational Lability of Lipases Observed in the Absence of an Oil-Water Interface: Crystallographic Studies of Enzymes from the Fungi Humicola lanuginosa and Rhizopus delemar, J. Lipid Res. 35, 524-534.
13. Rubin, B. (1994) Grease Pit Chemistry Exposed, Structural Biology 1, 568-572.
14. Joerger, R.D., and Haas, M.J. (1994) Alteration of Chain Length Selectivity of a Rhizopus delemar Lipase Through Site-Directed Mutagenesis, Lipids 29, 377-384.
15. Brzozowski, A.M., Derewenda, U., Derewenda, Z.S., Dodson, G.G., Lawson, D.M., Turkenburg, J.P., Bjorkling, F., Huge-Jensen, B., Patkar, S.A., and Thim, L. (1991) A Model for Interfacial Activation in Lipases from the Structure of a Fungal Lipase-Inhibitor Complex, Nature 351, 491-494.
16. Derewenda, Z.S., Derewenda, U., and Dodson, G.G. (1992) The Crystal and Molecular Structure of the Rhizomucor miehei Triacylglyceride Lipase at 1.9 Å Resolution, J. Mol. Biol. 227, 818-839.
17. Klein, R.R., and Salvucci, M.E. (1992) Photoaffinity Labeling of Mature and Precursor Forms of the Small Subunit of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase After Expression in Escherichia coli, Plant Physiol. 98, 546-553.
18. Sturdier, F.W., Rosenberg, A.H., Dunn, J.J., and Dubendorff, J.W. (1991) Use of T7 RNA Polymerase to Direct the Expression of Cloned Genes. Novagen (Technical Bulletin), Madison WI.
19. Kouker, G., and Jaeger, K.E. (1987) Specific and Sensitive Plate Assay for Bacterial Lipases, App. Environ. Microbiol. 53, 211-213.
20. Haas, M.J., Esposito, D., and Cichowicz, D.J. (1995) A Software Package to Streamline the Titrimetric Determination of Lipase Activity, J. Am. Oil Chem. Soc. 72, 1405-1406.
21. Uppenberg, J., Öhrner, N., Norin, M., Hult, K., Kleywegt, G.J., Patkar, S., Waagen, V., Anthonsen, T., and Jones, T.A. (1995) Crystallographic and Molecular-Modeling Studies of Lipase B from Candida antarctica Reveal a Stereospecificity Pocket for Secondary Alcohols, Biochemistry 34, 16838-16851.
22. Morrissey, J.H. (1981) Silver Stain for Proteins in Polyacrylamide Gels: A Modified Procedure with Enhanced Uniform Sensitivity, Anal. Biochem. 117, 307-310.
23. Holmquist, M., Clausen, I.G., Patkar, S., Svendsen, A., and Hult, K. (1995) Probing a Functional Role of Glu87 and Trp89 in the Lid of Humicola lanuginosa Lipase Through Transesterification Reactions in Organic Solvent, J. Protein Chem. 14, 217-224.
24. Schmid, R.D., Menge, U., Schomburg, D., and Spener, F. (1995) Towards Novel Biocatalysts via Protein Design: The Case of Lipases, FEMS Microbiol. Rev. 16, 253-257.
25. Delagrave, S., Goldman, E.R., and Youvan, D.C. (1993) Recursive Ensemble Mutagenesis, Protein Eng. 6, 327-331.
26. Chen, K., and Arnold, F.H. (1993) Tuning the Activity of an Enzyme for Unusual Environments: Sequential Random Mutagenesis of Subtilisin E for Catalysis in Dimethylformamide, Proc. Natl. Acad. Sci. USA 90, 5618-5622.
27. Stemmer, W.P.C. (1995) Searching Sequence Space, Biotechnology 13, 549-553.