Directed evolution of an esterase for the stereoselective resolution of a key intermediate in the synthesis of epothilones

Biotechnology and Bioengineering

Volumn 58, Issue 5, 1998, Pages 554-559

  Bornscheuer, Uwe T ^a   Altenbuchner, Josef ^a   Meyer, Hartmut H ^b  

^a UNIVERSITY OF STUTTGART   (Germany)

^b LEIBNIZ UNIVERSITY HANNOVER   (Germany)

Author keywords

Directed evolution; Epothilon; Esterase; Mutator strain; Pseudomonas fluorescens; Stereoselectivity

Indexed keywords

CATALYSIS; ENZYME KINETICS; ESTERS; HYDROLYSIS; MUTAGENESIS; PH; SYNTHESIS (CHEMICAL);

BIOTRANSFORMATIONS; EPOTHILONES; ESTERASE; MUTATOR STRAIN; PSEUDOMONAS FLUORESCENCE; STEREOSELECTIVITY;

CLONING;

EPOTHILONE B; ESTERASE;

ARTICLE; GENE MUTATION; LIPID ANALYSIS; LIPOGENESIS; LIPOLYSIS; NONHUMAN; PSEUDOMONAS FLUORESCENS; STEREOCHEMISTRY;

ANTINEOPLASTIC AGENTS; BIOTRANSFORMATION; CLONING, MOLECULAR; EPOTHILONES; EPOXY COMPOUNDS; ESCHERICHIA COLI; ESTERASES; PLASMIDS; PSEUDOMONAS FLUORESCENS; RECOMBINANT PROTEINS; STEREOISOMERISM; SUBSTRATE SPECIFICITY; THIAZOLES;

PSEUDOMONAS; PSEUDOMONAS FLUORESCENS; VIOLA (ANGIOSPERM);

EID: 0032486517     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19980605)58:5<554::AID-BIT12>3.0.CO;2-B     Document Type: Article

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