Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes

Nature Protocols

Volumn 2, Issue 4, 2007, Pages 891-903

  Reetz, Manfred T ^a   Carballeira, José Daniel ^a  

^a MAX PLANCK INSTITUT FÜR KOHLENFORSCHUNG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ACID LIPASE; BACTERIAL ENZYME;

ARTICLE; BACILLUS SUBTILIS; CATALYSIS; CODON USAGE; DIRECTED MOLECULAR EVOLUTION; ENANTIOSELECTIVITY; ENZYME ANALYSIS; ENZYME STRUCTURE; FUNCTIONAL PROTEOMICS; GENE LIBRARY; ITERATIVE SATURATION MUTAGENESIS; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN STABILITY; THERMOSTABILITY; THREE DIMENSIONAL IMAGING;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; CODON; DIRECTED MOLECULAR EVOLUTION; ENZYME STABILITY; ENZYMES; LIPASE; MODELS, MOLECULAR; MUTAGENESIS; MUTAGENESIS, SITE-DIRECTED; PROTEIN STRUCTURE, TERTIARY;

BACILLUS SUBTILIS;

EID: 34248567845     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2007.72     Document Type: Article

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