Directed evolution of a thermostable esterase

Proceedings of the National Academy of Sciences of the United States of America

Volumn 95, Issue 22, 1998, Pages 12809-12813

  Giver, Lori ^b   Gershenson, Anne ^b   Freskgard, Per Ola ^a,b   Arnold, Frances H ^b  

^a NOVO NORDISK A S   (Denmark)

^b California Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

4 NITROBENZYL ESTERASE; BACTERIAL ENZYME; ESTERASE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS SUBTILIS; CATALYSIS; CONFORMATIONAL TRANSITION; ENZYME ACTIVITY; ENZYME DENATURATION; ENZYME STABILITY; GENE DUPLICATION; GENE MUTATION; NATURAL SELECTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; TEMPERATURE SENSITIVITY; THERMOSTABILITY;

AMINO ACID SUBSTITUTION; BACILLUS SUBTILIS; CARBOXYLIC ESTER HYDROLASES; DIRECTED MOLECULAR EVOLUTION; ENZYME STABILITY; HEAT; KINETICS; MODELS, MOLECULAR; MUTAGENESIS; PLASMIDS; POINT MUTATION; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS); PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS);

EID: 0032573217     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.95.22.12809     Document Type: Article

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