Iterative saturation mutagenesis on the basis of b factors as a strategy for increasing protein thermostability

Angewandte Chemie - International Edition

Volumn 45, Issue 46, 2006, Pages 7745-7751

  Reetz, Manfred T ^a   Carballeira, José D ^a   Vogel, Andreas ^a  

^a MAX PLANCK INSTITUT FÜR KOHLENFORSCHUNG   (Germany)

Author keywords

Biotransformation; Directed evolution; Enzymes; Protein engineering; Protein stability

Indexed keywords

ITERATIVE METHODS; MUTAGENESIS; SATURATION (MATERIALS COMPOSITION); THERMAL EFFECTS; THERMODYNAMIC STABILITY;

BIOTRANSFORMATION; DIRECTED EVOLUTION; PROTEIN ENGINEERING; PROTEIN STABILITY;

PROTEINS;

BACTERIAL PROTEIN; LIPA PROTEIN, BACTERIA; PROTEIN;

ARTICLE; BACILLUS SUBTILIS; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; KINETICS; METHODOLOGY; MUTAGENESIS; PHYSIOLOGY; PROTEIN DENATURATION; PROTEIN TERTIARY STRUCTURE; TEMPERATURE; THERMODYNAMICS; X RAY CRYSTALLOGRAPHY;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; DIRECTED MOLECULAR EVOLUTION; ENZYME STABILITY; KINETICS; MODELS, MOLECULAR; MUTAGENESIS; PROTEIN DENATURATION; PROTEIN STRUCTURE, TERTIARY; PROTEINS; SUBSTRATE SPECIFICITY; TEMPERATURE; THERMODYNAMICS;

EID: 33845288649     PISSN: 14337851     EISSN: None     Source Type: Journal    
DOI: 10.1002/anie.200602795     Document Type: Article

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