A method for high-throughput screening of enantioselective catalysts

Angewandte Chemie - International Edition

Volumn 38, Issue 12, 1999, Pages 1758-1761

  Reetz, Manfred T ^a   Becker, Michael H ^a   Klein, Heinz Werner ^a   Stöckigt, Detlef ^a  

^a MAX PLANCK INSTITUT FÜR KOHLENFORSCHUNG   (Germany)

Author keywords

Asymmetric catalysis; Combinatorial chemistry; Enzyme catalysis; High throughput screening; Mass spectrometry

Indexed keywords

ARTICLE; CATALYST; CHEMICAL REACTION; ENANTIOMER; HYDROLYSIS; ISOTOPE LABELING; MASS SPECTROMETRY;

EID: 0033553835     PISSN: 14337851     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1521-3773(19990614)38:12<1758::AID-ANIE1758>3.0.CO;2-8     Document Type: Article

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42. [9a] to the determination of ee values in high-throughput screening. However, this requires an additional step (derivatization) and an excess of a 1:1 mixture of the pseudo-enantiomeric reagent as well as the assumption that conversion is complete without any enrichment of one of the diastereomers.
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45. +, Figure 1). The ratios of the peak intensities (and thus the ee values of the synthetic mixtures of 15 and 16) were obtained automatically using a macro to submit the data from the m/z intensity table of each measurement to an Excel spread sheet.
46. All enzyme-catalyzed hydrolytic reactions and esterifications were performed in deep-well microtiter plates (total volume 1.2 mL). For ESI-MS analysis a defined volume of the resulting product mixtures was extracted with diethyl ether. The extracts were automatically transferred in microtiter plates and diluted with methanol to a final concentration of 0.5-2.0 mM. The microtiter plates were placed in an automated sample manager (Scheme 2) equipped with a Rheodyne port for the injections.
47. M. T. Reetz, M. H. Becker, H.-W. Klein, D. Stöckigt, 1999, patent application submitted.