Immobilization of enzymes in microtiter plate scale

Biotechnology Journal

Volumn 1, Issue 5, 2006, Pages 582-587

  Brandt, Bettina ^a   Hidalgo, Aurelio ^a   Bornscheuer, Uwe T ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

Author keywords

Esterase; High throughput screening; Immobilization; Microtiter plate

Indexed keywords

1-PROPANOL; 96-WELL MICROTITER PLATE; ALCOHOLYSIS; ANHYDROUS CONDITIONS; COVALENT BONDING; ESTERASE; HIGH-THROUGHPUT SCREENING; IMMOBILIZATION METHOD; MICROTITER PLATES; P-NITROPHENYL ACETATE; TEST REACTIONS;

ADSORPTION; ESTERS; RADIOACTIVE WASTE VITRIFICATION;

ENZYME IMMOBILIZATION;

ESTERASE; IMMOBILIZED ENZYME;

ADSORPTION; ARTICLE; BIOASSAY; CHEMISTRY; ENZYME ACTIVATION; METHODOLOGY; TITRIMETRY;

ADSORPTION; BIOLOGICAL ASSAY; ENZYME ACTIVATION; ENZYMES, IMMOBILIZED; ESTERASES; TITRIMETRY;

EID: 33846189983     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.200600031     Document Type: Article

References (30)

1. Tischer, W., Kasche, V., Immobilized enzymes: crystals or carriers? Trends Biotechnol. 1999, 17, 326-335.
2. Schmid, A., Dordick, J.S., Hauer, B., Kiener, A. et al., Industrial biocatalysis today and tomorrow. Nature 2001, 409, 258-268.
3. Yamada, H., Kobayashi, M., Nitrile hydratase and its application to industrial production of acrylamide. Biosci. Biotechnol. Bioeng. 1996, 60, 1391-1400.
4. Klyosov, A.A., report number V-89-61316 UNIDO/IPTC 93, United Nations, 1989, p. 111.
5. Bornscheuer, U.T., Immobilizing enzymes: how to create more suitable biocatalysts. Angew. Chem. Int. Ed. 2003, 42, 3336-3337.
6. Cao, L., Carrier-bound immobilized enzymes, Wiley-VCH, Weinheim, 2005.
7. Handelsman, J., Metagenomics: application of genomics to uncultured microorganisms. Microbiol. Mol. Biol. Rev. 2004, 68, 669-685.
8. Handelsman, J., Sorting out metagenomes. Nat. Biotechnol. 2005, 23, 38-39.
9. Lorenz, P., Eck, J., Screening for novel industrial biocatalysts. Eng. Life Sci. 2004, 4, 501-504.
10. Uchiyama, T., Takashi, A., Ikemura, T., Watanabe, K., Substrate-induced gene-expression screening of environmental metagenome libraries for isolation of catabolic genes. Nat. Biotechnol. 2005, 23, 88-93.
11. Short, J.M., Molecular evolution: recombinant approaches for accessing biodiversity. Nat. Biotechnol. 1997, 15, 1322-1323.
12. Robertson, D.E., Chaplin, J.A., DeSantis, G., Podar, M. et al., Exploring nitrilase sequence space for enantioselective catalysis. Appl. Environ. Microbiol. 2004, 70, 2429-2436.
13. Betancor, L., Hidalgo, A., Fernandez-Lorente, G., Mateo, C. et al., Use of physicochemical tools to determine the choice of optimal enzyme: stabilization of D-amino acid oxidase. Biotechnol. Prog. 2003, 19, 784-788.
14. Fernandez-Lorente, G., Fernandez-Lafuente, R., Palomo, J.M., Mateo, C. et al., Biocatalyst engineering exerts a dramatic effect on selectivity of hydrolysis catalyzed by immobilized lipases in aqueous medium. J. Mol. Catal. B: Enzymatic 2001, 11, 649-656.
15. Palomo, J.M., Muñoz, G., Fernandez-Lorente, G., Mateo, C. et al., Modulation of Mucor miehei lipase properties via directed immobilization on different heterofunctional epoxi resins. Hydrolytic resolution of (R, S)-2-butyroyl-2-phenylacetic acid. J. Mol. Catal. B: Enzymatic 2003, 21, 201-210.
16. Palomo, J.M., Fernandez-Lorente, G., Mateo, C., Ortiz, C. et al., Modulation of the enantioselectivity of lipases via controlled immobilization and mediumm engineering: hydrolytic resolution of mandelic acid esters. Enzyme Microb. Technol. 2002, 31, 775-783.
17. Bornscheuer, U.T., Kazlauskas, R.J., Hydrolases in Organic Synthesis - Regio- and Stereoselective Biotransformations, 2nd edn., Wiley-VCH, Weinheim 2005.
18. Krebsfänger, N., Zocher, F., Altenbuchner, J., Bornscheuer, U.T., Characterization, and enantioselectivity of a recombinant esterase from Pseudomonas fluorescens. Enzyme Microb. Technol. 1998, 21, 641-646.
19. Khalameyzer, V., Bornscheuer, U.T., Overexpression and characterization of an esterase from Streptomyces diastatochromogenes. Biotechnol. Lett. 1999, 21, 101-104.
20. Henke, E., Bornscheuer, U.T., Schmid, R.D., Pleiss, J., A molecular mechanism of enantiorecognition of tertiary alcohols by carboxylesterases. ChemBioChem 2003, 4, 485-493.
21. Krishna, H.S., Persson, M.M., Bornscheuer, U.T., Enantioselective transesterification of a tertiary alcohol by lipase A from Candida antarctica. Tetrahedron: Asymmetry 2002, 13, 2693-2696.
22. Kreiner, M., Moore, B.D., Parker, M.C., Enzyme-coated micro-crystals: a 1-step method for high activity biocatalyst preparation. Chem. Commun. 2001, 1096-1097.
23. López-Serrano, P., Cao, L., van Rantwijk, F., Sheldon, R., Cross-linked enzyme aggregates with enhanced activity: application to lipases. Biotechnol. Lett. 2002, 24, 1379-1383.
24. Guisan, J.M., Bastida, A., Blanco, R.M., Fernandez-Lafuente, R., Garcia-Junceda, E., Immobilization of enzymes on glyoxyl agarose, in: Bickerstaff, G.F. (Ed.), Immobilization of Enzymes and Cells, Humana Press, Totowa 1997, pp. 277-288.
25. Mateo, C., Palomo, J.M., van Langen, L.M., van Rantwijk, F., Sheldon, R.A., A new, mild cross-linking methodology to prepare crosslinked enzyme aggregates. Biotechnol. Bioeng. 2004, 86, 273-276.
26. Lopez-Serrano, P., Cao, L., van Rantwijk, F., Sheldon, R., Cross-linked enzyme aggregates with enhanced activity: application to lipases. Biotechnol. Lett. 2002, 24, 1379-1383.
27. Mateo, C., Fernandez-Lorente, G., Abian, O., Fernandez-Lafuente, R., Guisan, J.M., Multifunctional epoxy supports: a new tool to improve the covalent immobilization of proteins. The promotion of physical adsorptions of proteins on the supports before their covalent linkage. Biomacromolecules 2000, 1, 739-745.
28. Tischer, W., Wedekind, F., Immobilized enzymes: methods and application. Topics Curr. Chem. 1998, 200, 95-126.
29. Abian, O., Mateo, C., Fernandez-Lorente, G., Palomo, J.M. et al., Stabilization of immobilized enzymes against water-soluble organic cosolvents and generation of hyper-hydrophilic micro-environments surrounding enzyme molecules. Biocatal. Biotrans. 2001, 19, 489-504.
30. Jokela, J., Pastinen, O., Leisola, M., Isomerization of pentose and hexose sugars by an enzyme reactor packed with cross-linked xylose isomerase crystals. Enzyme Microb. Technol. 2002, 31, 67-76.