Inverting enantioselectivity of Burkholderia cepacia KWI-56 lipase by combinatorial mutation and high-throughput screening using single-molecule PCR and in vitro expression

Journal of Molecular Biology

Volumn 331, Issue 3, 2003, Pages 585-592

  Koga, Yuichi ^a   Kato, Katsuya ^b   Nakano, Hideo ^c   Yamane, Tsuneo ^c  

^a NEW ENERGY AND INDUSTRIAL TECHNOLOGY DEVELOPMENT ORGANIZATION   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^c NAGOYA UNIVERSITY   (Japan)

Author keywords

Enantioselectivity; In vitro expression; Lipase; SIMPLEX; Single molecule PCR

Indexed keywords

AMINO ACID; BACTERIAL ENZYME; BUTYRIC ACID DERIVATIVE;

AMINO ACID SUBSTITUTION; ARTICLE; BURKHOLDERIA CEPACIA; CONTROLLED STUDY; ENANTIOSELECTIVITY; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SUBSTRATE; GENE AMPLIFICATION; GENE EXPRESSION; GENE LIBRARY; GENE MUTATION; GENETIC VARIABILITY; HYDROPHOBICITY; IN VITRO STUDY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TRANSCRIPTION REGULATION; TRANSLATION INITIATION; WILD TYPE;

BURKHOLDERIA; BURKHOLDERIA CEPACIA;

EID: 0042665295     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-2836(03)00782-4     Document Type: Article

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