In vitro selection of enzymatically active lipase variants from phage libraries using a mechanism-based inhibitor

Gene

Volumn 272, Issue 1-2, 2001, Pages 267-274

  Danielsen, Steffen ^a   Eklund, Malin ^b   Deussen, Heinz Josef ^a   Gräslund, Torbjörn ^b   Nygren, Per Åke ^b   Borchert, Torben V ^a  

^a NOVOZYMES A S   (Denmark)

^b ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

Author keywords

Affinity fusion; Directed evolution; Escherichia coli enzyme; Library; Lipolase; Phage display; Thermomyces lanuginosa

Indexed keywords

AMINO ACID; COAT PROTEIN; DETERGENT; SERINE; THREONINE; TRIACYLGLYCEROL LIPASE;

ARTICLE; BACTERIOPHAGE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE MUTATION; GENETIC ENGINEERING; IN VITRO STUDY; MOLECULAR CLONING; NONHUMAN; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RANDOMIZATION;

BACTERIOPHAGES; COMPUTER GRAPHICS; ESCHERICHIA COLI; GENE LIBRARY; LIPASE; MITOSPORIC FUNGI; MODELS, MOLECULAR; VARIATION (GENETICS);

COLI PHAGE; ESCHERICHIA COLI; THERMOMYCES LANUGINOSUS;

EID: 0035844981     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(01)00527-3     Document Type: Article

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