Anchored periplasmic expression, a versatile technology for the isolation of high-affinity antibodies from Escherichia coli-expressed libraries

Proceedings of the National Academy of Sciences of the United States of America

Volumn 101, Issue 25, 2004, Pages 9193-9198

  Harvey, Barrett R ^a   Georgiou, George ^a,b   Hayhurst, Andrew ^a,b   Jeong, Ki Jun ^a,b   Iverson, Brent L ^a   Rogers, Geoffrey K ^b  

^a The University of Texas at Austin   (United States)

^b UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ANTIGEN; BACTERIAL PROTEIN; BACTERIUM ANTIBODY; BINDING PROTEIN; COAT PROTEIN; DNA; EDETIC ACID; GREEN FLUORESCENT PROTEIN; LIGAND; LIPOPROTEIN; LYSOZYME; MEMBRANE PROTEIN; NEUTRALIZING ANTIBODY; PERIPLASMIC PROTEIN; PROTEIN NLPA; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANCHORED PERIPLASMIC EXPRESSION; ANTIBODY AFFINITY; ANTIBODY DETECTION; ARTICLE; BACILLUS ANTHRACIS; BACTERIAL MEMBRANE; BACTERIOPHAGE M13; CARBOXY TERMINAL SEQUENCE; CELL LABELING; COMBINATORIAL LIBRARY; CYTOPLASM; ESCHERICHIA COLI; FLOW CYTOMETRY; FLUORESCENCE; LIGAND BINDING; NONHUMAN; PHAGE DISPLAY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN ISOLATION; TECHNOLOGY;

ANTIBODIES, BACTERIAL; ANTIGENS, BACTERIAL; BACILLUS ANTHRACIS; BASE SEQUENCE; DNA PRIMERS; ESCHERICHIA COLI; FLOW CYTOMETRY; GENE EXPRESSION REGULATION, BACTERIAL; GENE LIBRARY; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; PERIPLASM; POLYMERASE CHAIN REACTION; RECOMBINANT FUSION PROTEINS;

BACILLUS ANTHRACIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 3042647616     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.0400187101     Document Type: Article

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