Function-based isolation of novel enzymes from a large library

Nature Biotechnology

Volumn 18, Issue 10 SUPPL., 2000, Pages 1071-1074

  Olsen, Mark J ^a   Stephens, Daren ^a,b   Griffiths, Devin ^a   Daugherty, Patrick ^b,c   Georgiou, George ^a,b   Iverson, Brent L ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^b The University of Texas at Austin   (United States)

^c USA   (United States)

Author keywords

Directed enzyme evolution; Flow cytometry; Fluorescence

Indexed keywords

BACTERIAL ENZYME; SERINE PROTEINASE;

AMINO ACID SEQUENCE; ANALYTIC METHOD; ARTICLE; CELL SURFACE; ENERGY TRANSFER; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ISOLATION; ENZYME SUBSTRATE; ESCHERICHIA COLI; FLUORESCENCE ACTIVATED CELL SORTER; GENE LIBRARY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; QUANTITATIVE ASSAY; SCREENING; SEQUENCE ANALYSIS;

BORON COMPOUNDS; CATALYSIS; CLONING, MOLECULAR; ENERGY TRANSFER; ENZYMES; ESCHERICHIA COLI; FLOW CYTOMETRY; FLUORESCENCE; FLUORESCENT DYES; KINETICS; MUTATION; PEPTIDE LIBRARY; RHODAMINES; SERINE ENDOPEPTIDASES; SUBSTRATE SPECIFICITY; VARIATION (GENETICS);

EID: 0033772183     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/80267     Document Type: Article

References (10)

1. Kast, P. & Hilvert, D. 3D structural information as a guide to protein engineering using genetic selection. Curr. Opin. Struct. Biol. 7, 470-479 (1997).
2. Arnold, F.H. & Volkov, AA Directed evolution of biocatalysts. Curr. Opin. Chem. Biol. 3, 54-59 (1999).
3. Stemmer, W.P.C. DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution. Nature 370, 389-391 (1994).
4. Crameri, A., Raillard, S.A., Bermudez, E. & Stemmer, W.P.C. DNA shuffling of a family of genes from diverse species accelerates directed evolution. Nature 391, 283-291 (1998).
5. Joo, H., Lin, Z. & Arnold, F.H. Laboratory evolution of peroxide-mediated cytochrome P450 hydroxylation. Nature 399, 670-673 (1998).
6. Matsumura, I., Wallingford, J.B., Surana, N.K., Vize, P.D. & Ellington, A.D. Directed evolution of the surface chemistry of the reporter enzyme beta-glucuronidase. Nat. Biotechnol. 17, 696-701 (1999).
7. MacBeath, G., Kast, P. & Hilvert, D. Redesigning enzyme topology by directed evolution. Science 279, 1958-1961 (1998).
8. Cantu, C., Huang, W. & Palzkill, T, Cephalosporin substrate specificity determinants of TEM-1 beta-lactamase. J. Biol. Chem. 272, 29144-29150 (1997).
9. Smiley, J.A. & Benkovic, S.J. Selection of catalytic antibodies for a biosynthetic reaction from a combinatorial cDNA library by complementation of an auxotrophic Escherichia coli: antibodies for orotate decarboxylation. Proc. Natl. Acad. Sci. USA 91, 8319-8323 (1994).
10. Christians, F.C. & Loeb, L.A. Novel human DNA alkyltransferases obtained by random substitution and genetic selection in bacteria. Proc. Natl. Acad. Sci. USA 93, 6124-6128 (1996).