Directed evolution of an esterase: Screening of enzyme libraries based on pH-Indicators and a growth assay

Bioorganic and Medicinal Chemistry

Volumn 7, Issue 10, 1999, Pages 2169-2173

  Bornscheuer, Uwe T ^a   Altenbuchner, Josef ^a   Meyer, Hartmut H ^b  

^a UNIVERSITY OF STUTTGART   (Germany)

^b LEIBNIZ UNIVERSITY HANNOVER   (Germany)

Author keywords

3 hydroxy ester; Directed evolution; Esterase; Growth assay; pH indicator

Indexed keywords

ESTERASE;

ARTICLE; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME SPECIFICITY; GENE MUTATION; PH; PSEUDOMONAS FLUORESCENS; SCREENING; STEREOCHEMISTRY;

CELL DIVISION; ESCHERICHIA COLI; ESTERASES; HYDROGEN-ION CONCENTRATION; INDICATORS AND REAGENTS; MODELS, MOLECULAR; MUTAGENESIS; MUTATION; PEPTIDE LIBRARY; PLASMIDS; PROMOTER REGIONS (GENETICS); PROTEIN CONFORMATION; PROTEIN ENGINEERING; PSEUDOMONAS FLUORESCENS; RECOMBINANT PROTEINS; RHAMNOSE; SEQUENCE ANALYSIS; SUBSTRATE SPECIFICITY;

EID: 0032828603     PISSN: 09680896     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0968-0896(99)00147-9     Document Type: Article

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