Directed evolution of an enantioselective lipase

Chemistry and Biology

Volumn 7, Issue 9, 2000, Pages 709-718

  Liebeton, Klaus ^a,b   Zonta, Albin ^b   Schimossek, Klaus ^b   Nardini, Marco ^c   Lang, Dietmar ^c   Dijkstra, Bauke W ^c   Reetz, Manfred T ^b   Jaeger, Karl Erich ^a  

^a RUHR UNIVERSITY BOCHUM   (Germany)

^b MAX PLANCK INSTITUT FÜR KOHLENFORSCHUNG   (Germany)

^c UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

Directed evolution; Enantioselectivity; Pseudomonas aeruginosa lipase; Random mutagenesis; Saturation mutagenesis

Indexed keywords

2 METHYLDECANOIC ACID 4 NITROPHENOL ESTER; AMINO ACID SUBSTITUTION; CHIRALITY; ENANTIOSELECTIVITY; MUTAGENESIS; POLYMERASE CHAIN REACTION; PSEUDOMONAS AERUGINOSA; TRIACYLGLYCEROL LIPASE; WILD RELATIVE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; DIRECTED MOLECULAR EVOLUTION; LIPASE; MODELS, MOLECULAR; MUTAGENESIS; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; PSEUDOMONAS AERUGINOSA; STEREOISOMERISM; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0033834791     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1074-5521(00)00015-6     Document Type: Article

References (52)

1. Directed evolution of biocatalysts
2. Methods for optimizing industrial enzymes by directed evolution
3. Directed evolution of enzyme catalysts
4. Creation of enantioselective biocatalysts for organic chemistry by in vitro evolution
5. Time-resolved IR-thermographic detection and screening of enantioselectivity in catalytic reactions
6. Method for high-throughput screening of enantioselective catalysts
7. Quantitative screening of hydrolase libraries using pH indicators: Identifying active and enantioselective hydrolases
8. Measurement of enantiomeric excess by kinetic resolution and mass spectrometry
9. Biotransformations with Lipases
10. Lipases: Interfacial enzymes with attractive applications
11. Microbial lipases form versatile tools for biotechnology
12. Bacterial lipases for biotechnological applications
13. Bacterial biocatalysts: Molecular biology, three-dimensional structures, and bio-technolgical applications of lipases
14. Crystal structure of Pseudomonas aeruginosa lipase in the open conformation: The prototype for family I.1 of bacterial lipases
15. A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction
16. Directed evolution of a paranitrobenzyl esterase for aqueous-organic solvents
17. DNA polymerase fidelity and the polymerase chain reaction
18. Directed evolution of a fungal peroxidase
19. Exploring nonnatural evolutionary pathways by saturation mutagenesis: Rapid improvement of protein function
20. Functional and nonfunctional mutations distinguished by random recombination of homologous genes
21. Rapid evolution of a protein in vitro by DNA shuffling
22. Optimization of DNA shuffling for high fidelity recombination
23. Molecular evolution by staggered extension process (StEP) in vitro recombination
24. Random-priming in vitro recombination: An effective tool for directed evolution
25. Recombination and chimeragenesis by in vitro heteroduplex formation and in vivo repair
26. Identification of residues essential for differential fatty acyl specificity of Geotrichum candidum lipases I and II
27. Insights into the molecular basis for fatty acyl specificities of lipases from Geotrichum candidum and Candida rugosa
28. Computational studies of the activation of lipases and the effect of a hydrophobic environment
29. Computational analysis of chain flexibility and fluctuations in Rhizomucor miehei lipase
30. Theoretical studies of Rhizomucor miehei lipase activation
31. Computer modeling of substrate binding to lipases from Rhizomucor miehei, Humicola lanuginosa, and Candida rugosa
32. Dynamics of proteins in different solvent systems: Analysis of essential motion in lipases
33. Essential dynamics of lipase binding sites: The effect of inhibitors of different chain length
34. The open conformation of a Pseudomonas lipase
35. The crystal structure of a triacylglycerol lipase from Pseudomonas cepacia reveals a highly open conformation in the absence of a bound inhibitor
36. Construction of improved vectors for protein production in Pseudomonas aeruginosa
37. - bacteria
38. Studies on transformation of Escherichia coli with plasmids
39. 2+ and heat
40. Improved method for PCR-mediated site-directed mutagenesis
41. Cleavage of the structural proteins during assembly of the head of bacteriophage T4
42. Glycogen, hyaluronate, and some other polysaccharides greatly enhance the formation of exo-lipase by Serratia marcescens
43. Improved methods for building protein models in electron density maps and the location of errors in these models
44. Crystallography and NMR system: A new software suite for macromolecular structure determination
45. The α/β hydrolase fold