High-throughput assays for lipases and esterases

Biomolecular Engineering

Volumn 22, Issue 1-3, 2005, Pages 51-56

  Schmidt, Marlen ^a   Bornscheuer, Uwe T ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

Author keywords

Assay; Directed evolution; Esterase; High throughput screening; Hydrolase; Lipase

Indexed keywords

BIOASSAY; MUTAGENESIS; SPECTROPHOTOMETRY; THROUGHPUT;

HIGH-THROUGHPUT ASSAYS; HIGH-THROUGHPUT SCREENING (HTS) SYSTEMS; LIPASES; MUTANT LIBRARIES;

ENZYMES;

ACETIC ACID; ADRENALIN; ESTERASE; TRIACYLGLYCEROL LIPASE;

DIRECTED MOLECULAR EVOLUTION; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SYNTHESIS; FLUOROMETRY; GENE LIBRARY; GENETIC VARIABILITY; HIGH THROUGHPUT SCREENING; MUTANT; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; RELIABILITY; REVIEW; SPECTROPHOTOMETRY;

EID: 18044397530     PISSN: 13890344     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bioeng.2004.09.004     Document Type: Review

References (38)

1. U.T. Bornscheuer, and R.J. Kazlauskas Hydrolases in organic synthesis - regio- and stereoselective biotransformations 1999 Wiley/VCH Weinheim
2. A. Liese, K. Seelbach, and C. Wandrey Industrial biotransformations 2000 Wiley/VCH Weinheim
3. F. Balkenhohl, K. Ditrich, B. Hauer, and W. Ladner J Prakt Chem 339 1997 381 384
4. U.T. Bornscheuer, and M. Pohl Curr Opin Chem Biol 5 2001 137 143
5. F.H. Arnold, and A.A. Volkov Curr Opin Chem Biol 3 1999 54 59
6. U.T. Bornscheuer Angew Chem. Int Ed 37 1998 3105 3108
7. U.T. Bornscheuer Biocat Biotrans 19 2001 84 96
8. F.H. Arnold, and G. Georgiou Methods in molecular biology 230 2003 Humana Press Totawa
9. F.H. Arnold, and G. Georgiou Methods in molecular biology 231 2003 Humana Press Totawa
10. R.C. Cadwell, and G.F. Joyce PCR Meth Appl 2 1992 28 33
11. U.T. Bornscheuer, J. Altenbuchner, and H.H. Meyer Biotechnol Bioeng 58 1998 554 559
12. A. Greener, M. Callahan, and B. Jerpseth Meth Mol Biol 57 1996 375 385
13. W.P.C. Stemmer Nature 370 1994 389 391
14. A.L. Kurtzman, S. Govindarajan, K. Vahle, J.T. Jones, V. Heinrichs, and P.A. Patten Curr Opin Biotechnol 12 2001 361 370
15. C. Neylon Nucl Acid Res 32 2004 1448 1459
16. M.T. Reetz, S. Wilensek, D. Zha, and K.-E. Jaeger Angew Chem Int Ed 40 2001 3589 3591
17. J.C. Moore, and F.H. Arnold Nat Biotechnol 14 1996 458 467
18. D. Wahler, and J.L. Reymond Curr Opin Biotechnol 12 2001 535 544
19. J.P. Goddard, and J.-L. Reymond Trends Biotechnol 22 2004 363 370
20. M.T. Reetz Angew Chem Int Ed 41 2002 1335 1338
21. M.T. Reetz, A. Zonta, K. Schimossek, K. Liebeton, and K.-E. Jaeger Angew Chem Int Ed Engl 36 1997 2830 2832
22. D.C. Demirjan, P.C. Shah, and F. Moris-Varas Top Curr Chem 200 1999 1 2
23. F. Badalassi, D. Wahler, G. Klein, P. Crotti, and J.-L. Reymond Angew Chem Int Ed Engl 39 2000 4067 4070
24. J. Grognux, and J.-L. Reymond Chem Bio Chem 5 2004 826 831
25. D. Wahler, F. Badalassi, P. Crotti, and J.-L. Reymond Angew Chem Int Ed 40 2001 4457 4460
26. E. Leroy, N. Bensel, and J.L. Reymond Bioorg Med Chem Lett 13 2003 2105 2108
27. E. Henke, and U.T. Bornscheuer Biol Chem 380 1999 1029 1033
28. M. Baumann, R. Stürmer, and U.T. Bornscheuer Angew Chem Int Ed 40 2001 4201 4204
29. M. Konarzycka-Bessler, and U.T. Bornscheuer Angew Chem Int Ed 42 2003 1418 1420
30. D. Wahler, and J.-L. Reymond Angew Chem Int Ed 41 2002 1129 1232
31. C.E. Humphrey, M.A.M. Easson, and N.J. Turner Chem Bio Chem 5 2004 1144 1148
32. L.E. Janes, and R.J. Kazlauskas J Org Chem 62 1997 4560 4561
33. L.E. Janes, A.C. Löwendahl, and R.J. Kazlauskas Chem Eur J 4 1998 2324 2331
34. U.T. Bornscheuer Nat Biotechnol 22 2004 1098 1099
35. B.D. Moore, L. Stevenson, A. Watt, S. Flitsch, N.J. Turner, and C. Cassidy Nat Biotechnol 22 2004 1133 1138
36. M.T. Reetz, M.H. Becker, H.W. Klein, and D. Stöckigt Angew Chem Int Ed Engl 38 1999 1758 1761
37. A. Fernandez-Gacio, M. Uguen, and J. Fastrez Trends Biotechnol 21 2003 408 414
38. A.D. Griffiths, and D.S. Tawfik Curr Opin Biotechnol 11 2000 338 353