Highly enantioselective kinetic resolution of two tertiary alcohols using mutants of an esterase from Bacillus subtilis

Protein Engineering, Design and Selection

Volumn 20, Issue 3, 2007, Pages 125-131

  Heinze, Birgit ^a   Kourist, Robert ^a   Fransson, Linda ^b   Hult, Karl ^b   Bornscheuer, Uwe T ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

^b ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

Author keywords

Enantioselectivity; Enzyme catalysis; Esterase; Rational protein design; Tertiary alcohol

Indexed keywords

ENZYME CATALYSIS; ESTERASE; RATIONAL PROTEIN DESIGN; TERTIARY ALCOHOL;

ALCOHOLS; BIOCATALYSTS; ENZYME ACTIVITY; ESTERS; HYDROLYSIS; REACTION KINETICS;

ENANTIOSELECTIVITY;

3 PHENYLBUT 1 YN 3 YLACETATE; ACETIC ACID DERIVATIVE; ALCOHOL; ARYLESTERASE; BACTERIAL PROTEIN; DIMETHYL SULFOXIDE; ENZYME VARIANT; METHYL GROUP; MUTANT PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS SUBTILIS; ENANTIOSELECTIVITY; ENZYME ACTIVE SITE; ENZYME PURIFICATION; ENZYME SUBSTRATE; HYDROLYSIS KINETICS; MOLECULAR MODEL; PRIORITY JOURNAL; PROTEIN ENGINEERING;

ACETATES; ALCOHOLS; AMINO ACID SUBSTITUTION; BACILLUS SUBTILIS; ESTERASES; KINETICS; STEREOISOMERISM;

BACILLUS SUBTILIS;

EID: 34047217953     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzm003     Document Type: Article

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