Improved enantioselectivity of a lipase by rational protein engineering

ChemBioChem

Volumn 2, Issue 10, 2001, Pages 766-770

  Rotticci, Didier ^a   Rotticci Mulder, Johanna C ^a   Denman, Stuart ^a   Norin, Torbjörn ^a   Hult, Karl ^a  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

Author keywords

Enzyme catalysis; Kinetic resolution; Lipases; Protein engineering; Rational design

Indexed keywords

LIPASE B, CANDIDA ANTARCTICA; TRIACYLGLYCEROL; TRIACYLGLYCEROL LIPASE; TRIBUTYRIN;

ARTICLE; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; ELECTRICITY; ENZYME SPECIFICITY; GENETICS; KINETICS; METABOLISM; METHODOLOGY; PROTEIN CONFORMATION; PROTEIN ENGINEERING; STEREOISOMERISM; THERMODYNAMICS;

BINDING SITES; ELECTROSTATICS; KINETICS; LIPASE; MODELS, MOLECULAR; PROTEIN CONFORMATION; PROTEIN ENGINEERING; STEREOISOMERISM; SUBSTRATE SPECIFICITY; THERMODYNAMICS; TRIGLYCERIDES;

EID: 0035496145     PISSN: 14394227     EISSN: None     Source Type: Journal    
DOI: 10.1002/1439-7633(20011001)2:10<766::AID-CBIC766>3.0.CO;2-K     Document Type: Article

References (4)

1. B. Schulze, M. G. Wubbolts, Curr. Opin. Biotechnol., 1999, 10, 609-615.
2. A. Liese, M. Villela Filho, Curr. Opin. Biotechnol. 1999, 10, 595-603.
3. K. Faber, Biotransformations in Organic Chemistry, Springer, Berlin, 1997.
4. Enzyme Catalysis in Organic Synthesis, Vol.I and II (Eds.: K. Drauz, H. Waldmann), VCH, Weinheim, 1995.