A high-throughput, low-volume enzyme assay on solid support

Analytical Chemistry

Volumn 77, Issue 2, 2005, Pages 373-377

  Babiak, Peter ^a   Reymond, Jean Louis ^a  

^a UNIVERSITY OF BERN   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME ASSAY; ENZYME SOLUTIONS; FLUOROGENIC SUBSTRATES; LIPASE;

ESTERS; HYDROLYSIS; PROTEINS; SILICA GEL; SUBSTRATES;

ENZYMES;

ENZYME; ESTERASE; SILICA GEL; TRIACYLGLYCEROL LIPASE;

ARTICLE; ENZYME ASSAY; FLUORESCENCE; HIGH THROUGHPUT SCREENING; HYDROLYSIS; QUANTITATIVE ASSAY; ROBOTICS;

AUTOANALYSIS; ENZYMES; ESTERASES; FLUORESCENT DYES; GLYCOSIDE HYDROLASES; LIPASE; PEPTIDE HYDROLASES; REPRODUCIBILITY OF RESULTS; SILICA GEL; SILICON DIOXIDE; SOLUBILITY; WATER;

EID: 12244253039     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac048611n     Document Type: Article

References (32)

1. Enzyme Assays: A Practical Approach; Eisenthal, R., Danson, M., Eds.; Oxford University Press: New York, 2002.
2. (b) Gul, S.; Sreedharan, S. K.; Brocklehurst, K. Enzyme Assays: Essential Data; John Wiley & Sons: Chichester, U.K., 1998.
3. (c) Reetz, M. T. Angew. Chem. 2001, 113, 292-320; Angew. Chem., Int. Ed. 2001, 40, 284-310.
4. (c) Reetz, M. T. Angew. Chem. 2001, 113, 292-320; Angew. Chem., Int. Ed. 2001, 40, 284-310.
5. (d) Wahler, D.; Reymond, J.-L. Curr. Opin. Chem. Biol. 2001, 5, 152-158.
6. (e) Wahler, D.; Reymond, J.-L. Curr. Opin. Biotechnol. 2001, 12, 535-544.
7. (f). Goddard, J.-P.; Reymond, J.-L. Trends Biotechnol. 2004, 22, 363-370.
8. Beisson, F.; Tiss, A.; Rivière, C.; Verger, R. Eur. J. Lipid Sci. Technol. 2000, 133-153.
9. (b) Wahler, D.; Reymond, J.-L. Angew. Chem., Int. Ed. 2002, 41, 1229.
10. (c) Wahler, D.; Boujard, O.; Lefèvre, F.; Reymond, J.-L. Tetrahedron 2004, 60, 703.
11. Lagarde, D.; Nguyen, H.-K.; Ravot, G.; Wahler, D.; Reymond, J.-L.; Hills, G.; Veit, T.; Lefevre, F. Org. Process. R. D. 2002, 6, 441.
12. (b) Nyfeler, E.; Grognux, J.; Wahler, D.; Reymond, J.-L. Helv. Chim. Acta 2003, 86, 2919.
13. (c) Gonzalez-Garcia, E. M.; Grognux, J.; Wahler, D.; Reymond, J.-L. Helv. Chim. Acta 2003, 86, 2458.
14. Badalassi, F.; Wahler, D.; Klein, G.; Crotti, P.; Reymond, J.-L. Angew. Chem., Int. Ed. 2000, 39, 4067.
15. (b) Grognux, J.; Wahler, D.; Nyfeler, E.; Reymond, J.-L. Tetrahedron Asymmetry 2004, 15, 2981.
16. Leroy, E.; Bensel, N.; Reymond, J.-L. Bioorg. Med. Chem. Lett. 2003, 13, 2105.
17. (b) Leroy, E.; Bensel, N.; Reymond, J.-L. Adv. Synth. Catal. 2003, 345, 859.
18. % v/v DMSO was added as cosolvent in the reference solution assay, which also showed a high level of nonspecific background hydrolysis.
19. Fluorescence reading of an empty silica gel plate showed a background fluorescence signal or up to 5% of the signal recorded when reading the assays.
20. Leroy, E.; Reymond, J.-L. Org. Lett. 1999, 1, 775-777.
21. (b) Gartenmann Dickson, L.; Leroy, E.; Reymond, J.-L. Org. Biomol. Chem. 2004, 2, 1217-1226.
22. Klein, G.; Reymond, J.-L. Angew. Chem., Int. Ed. 2001, 40, 1771.
23. (b) Dean, K. E. S.; Klein, G.; Renaudet, O.; Reymond, J.-L. Bioorg. Med. Chem. Lett. 2003, 10, 1653.
24. Wahler, D.; Badalassi, F.; Crotti, P.; Reymond, J.-L. Angew. Chem., Int. Ed. 2001, 40, 4457,
25. (b) Wahler, D.; Badalassi, F.; Crotti, P.; Reymond, J.-L. Chem. Eur. J. 2002, 8, 3211.
26. (c) Reymond, J.-L.; Wahler, D. ChemBioChem 2002, 3, 701-708.
27. (d) Grognux, J.; Reymond, J.-L. ChemBioChem 2004, 5, 826-831.
28. (e) Goddard, J.-P.; Reymond, J.-L. J. Am. Chem. Soc. 2004, 126, 11116-11117.
29. Salisbury, C. M.; Maly, D. J.; Ellman, J. A. J. Am. Chem. Soc. 2002, 50, 14868-14870.
30. (b) Zhu, Q.; Uttamchandani, M.; Li, D.; Lesaicherre, M. L.; Yao, S. Q. Org. Lett. 2003, 8, 1257-1260.
31. (c) Hauseman, B. T.; Huh, J. H.; Kron, S. J.; Mrksich, M. Nat. Biotechnol. 2002, 20, 270-274,
32. (d) Gosalia, D. N.; Diamond, S. L. Proc. Natl. Acad. Sci. U.S.A. 2003, 15, 8721-8726.