메뉴 건너뛰기




Volumn 6, Issue 5, 2013, Pages 579-603

Advances in mammalian cell line development technologies for recombinant protein production

Author keywords

Biopharmaceutical production; Cell line development; Clone screening; Protein expression

Indexed keywords

RECOMBINANT PROTEIN; X BOX BINDING PROTEIN 1; X LINKED INHIBITOR OF APOPTOSIS; ZINC FINGER NUCLEASE;

EID: 84876959581     PISSN: None     EISSN: 14248247     Source Type: Journal    
DOI: 10.3390/ph6050579     Document Type: Review
Times cited : (240)

References (135)
  • 2
    • 84856389498 scopus 로고    scopus 로고
    • CHO cells in biotechnology for production of recombinant proteins: Current state and further potential
    • Kim, J.Y.; Kim, Y.G.; Lee, G.M. CHO cells in biotechnology for production of recombinant proteins: Current state and further potential. Appl. Microbiol. Biotechnol. 2012, 93, 917-930.
    • (2012) Appl. Microbiol. Biotechnol , vol.93 , pp. 917-930
    • Kim, J.Y.1    Kim, Y.G.2    Lee, G.M.3
  • 3
    • 84860912187 scopus 로고    scopus 로고
    • Production platforms for biotherapeutic glycoproteins. Occurrence, impact, and challenges of non-human sialylation
    • Ghaderi, D.; Zhang, M.; Hurtado-Ziola, N.; Varki, A. Production platforms for biotherapeutic glycoproteins. Occurrence, impact, and challenges of non-human sialylation. Biotechnol. Genet. Eng. Rev. 2012, 28, 147-175.
    • (2012) Biotechnol. Genet. Eng. Rev , vol.28 , pp. 147-175
    • Ghaderi, D.1    Zhang, M.2    Hurtado-Ziola, N.3    Varki, A.4
  • 5
    • 84876996309 scopus 로고    scopus 로고
    • Accessed on 16 April 2013
    • TOP 30 Biologics 2011. Available online: http://www.pipelinereview.com/index.php/archive/ view/listid-1-la-merie-daily/mailid-35-La-Merie-Daily-TOP-30-Biologics-2011-new-free-report/tmpl-component (Accessed on 16 April 2013).
    • (2011) TOP 30 Biologics
  • 7
    • 0020400592 scopus 로고
    • Amplification and expression of sequences cotransfected with a modular dihydrofolate reductase complementary dna gene
    • Kaufman, R.J.; Sharp, P.A. Amplification and expression of sequences cotransfected with a modular dihydrofolate reductase complementary dna gene. J. Mol. Biol. 1982, 159, 601-621.
    • (1982) J. Mol. Biol , vol.159 , pp. 601-621
    • Kaufman, R.J.1    Sharp, P.A.2
  • 8
    • 0035313635 scopus 로고    scopus 로고
    • Industrial choices for protein production by large-scale cell culture
    • Chu, L.; Robinson, D.K. Industrial choices for protein production by large-scale cell culture. Curr. Opin. Biotechnol. 2001, 12, 180-187.
    • (2001) Curr. Opin. Biotechnol , vol.12 , pp. 180-187
    • Chu, L.1    Robinson, D.K.2
  • 9
    • 14744283551 scopus 로고
    • High-level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable marker
    • Bebbington, C.R.; Renner, G.; Thomson, S.; King, D.; Abrams, D.; Yarranton, G.T. High-level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable marker. Biotechnology (NY) 1992, 10, 169-175.
    • (1992) Biotechnology (NY) , vol.10 , pp. 169-175
    • Bebbington, C.R.1    Renner, G.2    Thomson, S.3    King, D.4    Abrams, D.5    Yarranton, G.T.6
  • 10
    • 35548990601 scopus 로고    scopus 로고
    • Selection methods for high-producing mammalian cell lines
    • Browne, S.M.; Al-Rubeai, M. Selection methods for high-producing mammalian cell lines. Trends Biotechnol. 2007, 25, 425-432.
    • (2007) Trends Biotechnol , vol.25 , pp. 425-432
    • Browne, S.M.1    Al-Rubeai, M.2
  • 12
    • 0001127374 scopus 로고
    • Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity
    • Urlaub, G.; Chasin, L.A. Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity. Proc. Natl. Acad. Sci. USA 1980, 77, 4216-4220.
    • (1980) Proc. Natl. Acad. Sci. USA , vol.77 , pp. 4216-4220
    • Urlaub, G.1    Chasin, L.A.2
  • 14
    • 84876981020 scopus 로고    scopus 로고
    • Accessed on 15 February
    • Lonza launches next generation GS gene expression system. Available online: http://www.lonza.com/ about-lonza/media-center/news/2012/120710-GS-System-e.aspx (Accessed on 15 February 2013).
    • (2013) Lonza Launches Next Generation GS Gene Expression System
  • 15
    • 0021735425 scopus 로고
    • Gene amplification in cultured animal cells
    • Schimke, R.T. Gene amplification in cultured animal cells. Cell 1984, 37, 705-713.
    • (1984) Cell , vol.37 , pp. 705-713
    • Schimke, R.T.1
  • 16
    • 0025884056 scopus 로고
    • Efficient selection for high-expression transfectants with a novel eukaryotic vector
    • Niwa, H.; Yamamura, K.; Miyazaki, J. Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 1991, 108, 193-199.
    • (1991) Gene , vol.108 , pp. 193-199
    • Niwa, H.1    Yamamura, K.2    Miyazaki, J.3
  • 17
    • 15944391987 scopus 로고    scopus 로고
    • Selection of high-producing CHO cells using NPT selection marker with reduced enzyme activity
    • Sautter, K.; Enenkel, B. Selection of high-producing CHO cells using NPT selection marker with reduced enzyme activity. Biotechnol. Bioeng. 2005, 89, 530-538.
    • (2005) Biotechnol. Bioeng , vol.89 , pp. 530-538
    • Sautter, K.1    Enenkel, B.2
  • 18
    • 2942533342 scopus 로고    scopus 로고
    • Illegitimate DNA integration in mammalian cells
    • Wurtele, H.; Little, K.C.; Chartrand, P. Illegitimate DNA integration in mammalian cells. Gene Ther. 2003, 10, 1791-1799.
    • (2003) Gene Ther , vol.10 , pp. 1791-1799
    • Wurtele, H.1    Little, K.C.2    Chartrand, P.3
  • 19
    • 17444369067 scopus 로고    scopus 로고
    • Remote control of gene transcription
    • West, A.G.; Fraser, P. Remote control of gene transcription. Hum. Mol. Genet. 2005, 14, R101-R111.
    • (2005) Hum. Mol. Genet , vol.14
    • West, A.G.1    Fraser, P.2
  • 20
    • 8344271026 scopus 로고    scopus 로고
    • Production of recombinant protein therapeutics in cultivated mammalian cells
    • Wurm, F.M. Production of recombinant protein therapeutics in cultivated mammalian cells. Nat. Biotechnol. 2004, 22, 1393-1398.
    • (2004) Nat. Biotechnol , vol.22 , pp. 1393-1398
    • Wurm, F.M.1
  • 22
    • 77954076709 scopus 로고    scopus 로고
    • Intraclonal protein expression heterogeneity in recombinant CHO cells
    • Pilbrough, W.; Munro, T.P.; Gray, P. Intraclonal protein expression heterogeneity in recombinant CHO cells. PLoS One 2009, 4, e8432.
    • (2009) PLoS One , vol.4
    • Pilbrough, W.1    Munro, T.P.2    Gray, P.3
  • 24
    • 34247526431 scopus 로고    scopus 로고
    • Protein-free transfection of CHO host cells with an IgG-fusion protein: Selection and characterization of stable high producers and comparison to conventionally transfected clones
    • Lattenmayer, C.; Loeschel, M.; Schriebl, K.; Steinfellner, W.; Sterovsky, T.; Trummer, E.; Vorauer-Uhl, K.; Muller, D.; Katinger, H.; Kunert, R. Protein-free transfection of CHO host cells with an IgG-fusion protein: Selection and characterization of stable high producers and comparison to conventionally transfected clones. Biotechnol. Bioeng. 2007, 96, 1118-1126.
    • (2007) Biotechnol. Bioeng , vol.96 , pp. 1118-1126
    • Lattenmayer, C.1    Loeschel, M.2    Schriebl, K.3    Steinfellner, W.4    Sterovsky, T.5    Trummer, E.6    Vorauer-Uhl, K.7    Muller, D.8    Katinger, H.9    Kunert, R.10
  • 25
    • 0002598959 scopus 로고    scopus 로고
    • Characterization of chimeric antibody producing CHO cells in the course of dihydrofolate reductase-mediated gene amplification and their stability in the absence of selective pressure
    • Kim, S.J.; Kim, N.S.; Ryu, C.J.; Hong, H.J.; Lee, G.M. Characterization of chimeric antibody producing CHO cells in the course of dihydrofolate reductase-mediated gene amplification and their stability in the absence of selective pressure. Biotechnol. Bioeng. 1998, 58, 73-84.
    • (1998) Biotechnol. Bioeng , vol.58 , pp. 73-84
    • Kim, S.J.1    Kim, N.S.2    Ryu, C.J.3    Hong, H.J.4    Lee, G.M.5
  • 26
    • 0035128469 scopus 로고    scopus 로고
    • Key determinants in the occurrence of clonal variation in humanized antibody expression of cho cells during dihydrofolate reductase mediated gene amplification
    • Kim, N.S.; Byun, T.H.; Lee, G.M. Key determinants in the occurrence of clonal variation in humanized antibody expression of cho cells during dihydrofolate reductase mediated gene amplification. Biotechnol. Prog. 2001, 17, 69-75.
    • (2001) Biotechnol. Prog , vol.17 , pp. 69-75
    • Kim, N.S.1    Byun, T.H.2    Lee, G.M.3
  • 27
    • 0022100495 scopus 로고
    • Coamplification and coexpression of human tissue-type plasminogen activator and murine dihydrofolate reductase sequences in Chinese hamster ovary cells
    • Kaufman, R.J.; Wasley, L.C.; Spiliotes, A.J.; Gossels, S.D.; Latt, S.A.; Larsen, G.R.; Kay, R.M. Coamplification and coexpression of human tissue-type plasminogen activator and murine dihydrofolate reductase sequences in Chinese hamster ovary cells. Mol. Cell. Biol. 1985, 5, 1750-1759.
    • (1985) Mol. Cell. Biol , vol.5 , pp. 1750-1759
    • Kaufman, R.J.1    Wasley, L.C.2    Spiliotes, A.J.3    Gossels, S.D.4    Latt, S.A.5    Larsen, G.R.6    Kay, R.M.7
  • 28
    • 60549102844 scopus 로고    scopus 로고
    • A study of monoclonal antibody-producing CHO cell lines: What makes a stable high producer
    • Chusainow, J.; Yang, Y.S.; Yeo, J.H.; Toh, P.C.; Asvadi, P.; Wong, N.S.; Yap, M.G. A study of monoclonal antibody-producing CHO cell lines: What makes a stable high producer? Biotechnol. Bioeng. 2009, 102, 1182-1196.
    • (2009) Biotechnol. Bioeng , vol.102 , pp. 1182-1196
    • Chusainow, J.1    Yang, Y.S.2    Yeo, J.H.3    Toh, P.C.4    Asvadi, P.5    Wong, N.S.6    Yap, M.G.7
  • 29
    • 0033038265 scopus 로고    scopus 로고
    • Genetic optimization of recombinant glycoprotein production by mammalian cells
    • Fussenegger, M.; Bailey, J.E.; Hauser, H.; Mueller, P.P. Genetic optimization of recombinant glycoprotein production by mammalian cells. Trends Biotechnol. 1999, 17, 35-42.
    • (1999) Trends Biotechnol , vol.17 , pp. 35-42
    • Fussenegger, M.1    Bailey, J.E.2    Hauser, H.3    Mueller, P.P.4
  • 31
    • 84855237715 scopus 로고    scopus 로고
    • IRES-mediated Tricistronic vectors for enhancing generation of high monoclonal antibody expressing CHO cell lines
    • Ho, S.C.; Bardor, M.; Feng, H.; Mariati S., Tong Y.W.; Song, Z, Yap, M.G.; Yang, Y. IRES-mediated Tricistronic vectors for enhancing generation of high monoclonal antibody expressing CHO cell lines. J. Biotechnol. 2012, 157, 130-139.
    • (2012) J. Biotechnol , vol.157 , pp. 130-139
    • Ho, S.C.1    Bardor, M.2    Feng, H.3    Mariati, S.4    Tong, Y.W.5    Song, Z.6    Yap, M.G.7    Yang, Y.8
  • 32
    • 0028877140 scopus 로고
    • Production of monoclonal antibodies in COS and CHO cells
    • Trill, J.J.; Shatzman, A.R.; Ganguly, S. Production of monoclonal antibodies in COS and CHO cells. Curr. Opin. Biotechnol. 1995, 6, 553-560.
    • (1995) Curr. Opin. Biotechnol , vol.6 , pp. 553-560
    • Trill, J.J.1    Shatzman, A.R.2    Ganguly, S.3
  • 33
    • 76849100565 scopus 로고    scopus 로고
    • Vector fragmentation: Characterizing vector integrity in transfected clones by Southern blotting
    • Ng, S.K.; Lin, W.; Sachdeva, R.; Wang, D.I.; Yap, M.G. Vector fragmentation: Characterizing vector integrity in transfected clones by Southern blotting. Biotechnol. Prog. 2010, 26, 11-20.
    • (2010) Biotechnol. Prog , vol.26 , pp. 11-20
    • Ng, S.K.1    Lin, W.2    Sachdeva, R.3    Wang, D.I.4    Yap, M.G.5
  • 34
    • 0026431613 scopus 로고
    • Improved vectors for stable expression of foreign genes in mammalian cells by use of the untranslated leader sequence from EMC virus
    • Kaufman, R.J.; Davies, M.V.; Wasley, L.C.; Michnick, D. Improved vectors for stable expression of foreign genes in mammalian cells by use of the untranslated leader sequence from EMC virus. Nucleic Acids Res. 1991, 19, 4485-4490.
    • (1991) Nucleic Acids Res , vol.19 , pp. 4485-4490
    • Kaufman, R.J.1    Davies, M.V.2    Wasley, L.C.3    Michnick, D.4
  • 35
    • 0030027934 scopus 로고    scopus 로고
    • Bicistronic vector for the creation of stable mammalian cell lines that predisposes all antibiotic-resistant cells to express recombinant protein
    • 106
    • Rees, S.; Coote, J.; Stables, J.; Goodson, S.; Harris, S.; Lee, M.G. Bicistronic vector for the creation of stable mammalian cell lines that predisposes all antibiotic-resistant cells to express recombinant protein. Biotechniques 1996, 20, 102-104, 106, 108-110.
    • (1996) Biotechniques , vol.20 , pp. 102-104
    • Rees, S.1    Coote, J.2    Stables, J.3    Goodson, S.4    Harris, S.5    Lee, M.G.6
  • 36
    • 0030569542 scopus 로고    scopus 로고
    • IRES bicistronic expression vectors for efficient creation of stable mammalian cell lines
    • Gurtu, V.; Yan, G.; Zhang, G. IRES bicistronic expression vectors for efficient creation of stable mammalian cell lines. Biochem. Biophys. Res. Commun. 1996, 229, 295-298.
    • (1996) Biochem. Biophys. Res. Commun , vol.229 , pp. 295-298
    • Gurtu, V.1    Yan, G.2    Zhang, G.3
  • 37
    • 0030695272 scopus 로고    scopus 로고
    • Expression of a recombinant monoclonal antibody from a bicistronic mRNA
    • Kolb, A.F.; Siddell, S.G. Expression of a recombinant monoclonal antibody from a bicistronic mRNA. Hybridoma 1997, 16, 421-426.
    • (1997) Hybridoma , vol.16 , pp. 421-426
    • Kolb, A.F.1    Siddell, S.G.2
  • 39
    • 84871556009 scopus 로고    scopus 로고
    • Production of Functional Soluble Dectin-1 Glycoprotein Using an IRES-Linked Destabilized-Dihydrofolate Reductase Expression Vector
    • Ng, S.K.; Tan, T.R.; Wang, Y.; Ng, D.; Goh, L.T.; Bardor, M.; Wong, V.V.; Lam, K.P. Production of Functional Soluble Dectin-1 Glycoprotein Using an IRES-Linked Destabilized-Dihydrofolate Reductase Expression Vector. PLoS One 2012, 7, e52785.
    • (2012) PLoS One , vol.7
    • Ng, S.K.1    Tan, T.R.2    Wang, Y.3    Ng, D.4    Goh, L.T.5    Bardor, M.6    Wong, V.V.7    Lam, K.P.8
  • 40
    • 0029127463 scopus 로고
    • Heterologous expression as a tool for gene identification and analysis
    • Gross, G.; Hauser, H. Heterologous expression as a tool for gene identification and analysis. J. Biotechnol. 1995, 41, 91-110.
    • (1995) J. Biotechnol , vol.41 , pp. 91-110
    • Gross, G.1    Hauser, H.2
  • 41
    • 78650197691 scopus 로고    scopus 로고
    • Improved recombinant protein yield using a codon deoptimized DHFR selectable marker in a CHEF1 expression plasmid
    • Westwood, A.D.; Rowe, D.A.; Clarke, H.R. Improved recombinant protein yield using a codon deoptimized DHFR selectable marker in a CHEF1 expression plasmid. Biotechnol. Prog. 2010, 26, 1558-1566.
    • (2010) Biotechnol. Prog , vol.26 , pp. 1558-1566
    • Westwood, A.D.1    Rowe, D.A.2    Clarke, H.R.3
  • 42
    • 34249741495 scopus 로고    scopus 로고
    • Application of destabilizing sequences on selection marker for improved recombinant protein productivity in CHO-DG44
    • Ng, S.K.; Wang, D.I.; Yap, M.G. Application of destabilizing sequences on selection marker for improved recombinant protein productivity in CHO-DG44. Metab. Eng. 2007, 9, 304-316.
    • (2007) Metab. Eng , vol.9 , pp. 304-316
    • Ng, S.K.1    Wang, D.I.2    Yap, M.G.3
  • 43
    • 0021675784 scopus 로고
    • Organization of the higher-order chromatin loop: Specific DNA attachment sites on nuclear scaffold
    • Mirkovitch, J.; Mirault, M.E.; Laemmli, U.K. Organization of the higher-order chromatin loop: Specific DNA attachment sites on nuclear scaffold. Cell 1984, 39, 223-232.
    • (1984) Cell , vol.39 , pp. 223-232
    • Mirkovitch, J.1    Mirault, M.E.2    Laemmli, U.K.3
  • 44
    • 0035504496 scopus 로고    scopus 로고
    • 5-Methylcytosine DNA glycosylase participates in the genome-wide loss of DNA methylation occurring during mouse myoblast differentiation
    • Jost, J.P.; Oakeley, E.J.; Zhu, B.; Benjamin, D.; Thiry, S.; Siegmann, M.; Jost, Y.C. 5-Methylcytosine DNA glycosylase participates in the genome-wide loss of DNA methylation occurring during mouse myoblast differentiation. Nucleic Acids Res. 2001, 29, 4452-4461.
    • (2001) Nucleic Acids Res , vol.29 , pp. 4452-4461
    • Jost, J.P.1    Oakeley, E.J.2    Zhu, B.3    Benjamin, D.4    Thiry, S.5    Siegmann, M.6    Jost, Y.C.7
  • 45
    • 0035942193 scopus 로고    scopus 로고
    • Overexpression of 5-methylcytosine DNA glycosylase in human embryonic kidney cells EcR293 demethylates the promoter of a hormone-regulated reporter gene
    • Zhu, B.; Benjamin, D.; Zheng, Y.; Angliker, H.; Thiry, S.; Siegmann, M.; Jost, J.P. Overexpression of 5-methylcytosine DNA glycosylase in human embryonic kidney cells EcR293 demethylates the promoter of a hormone-regulated reporter gene. Proc. Natl. Acad. Sci. USA 2001, 98, 5031-5036.
    • (2001) Proc. Natl. Acad. Sci. USA , vol.98 , pp. 5031-5036
    • Zhu, B.1    Benjamin, D.2    Zheng, Y.3    Angliker, H.4    Thiry, S.5    Siegmann, M.6    Jost, J.P.7
  • 47
    • 0033529654 scopus 로고    scopus 로고
    • The protein CTCF is required for the enhancer blocking activity of vertebrate insulators
    • Bell, A.C.; West, A.G.; Felsenfeld, G. The protein CTCF is required for the enhancer blocking activity of vertebrate insulators. Cell 1999, 98, 387-396.
    • (1999) Cell , vol.98 , pp. 387-396
    • Bell, A.C.1    West, A.G.2    Felsenfeld, G.3
  • 49
    • 21344449323 scopus 로고    scopus 로고
    • Use of the chicken lysozyme 5' matrix attachment region to generate high producer CHO cell lines
    • Girod, P.A.; Zahn-Zabal, M.; Mermod, N. Use of the chicken lysozyme 5' matrix attachment region to generate high producer CHO cell lines. Biotechnol. Bioeng. 2005, 91, 1-11.
    • (2005) Biotechnol. Bioeng , vol.91 , pp. 1-11
    • Girod, P.A.1    Zahn-Zabal, M.2    Mermod, N.3
  • 50
    • 0346094306 scopus 로고    scopus 로고
    • Improved recombinant gene expression in CHO cells using matrix attachment regions
    • Kim, J.M.; Kim, J.S.; Park, D.H.; Kang, H.S.; Yoon, J.; Baek, K.; Yoon, Y. Improved recombinant gene expression in CHO cells using matrix attachment regions. J. Biotechnol. 2004, 107, 95-105.
    • (2004) J. Biotechnol , vol.107 , pp. 95-105
    • Kim, J.M.1    Kim, J.S.2    Park, D.H.3    Kang, H.S.4    Yoon, J.5    Baek, K.6    Yoon, Y.7
  • 51
    • 20144374138 scopus 로고    scopus 로고
    • Efficient selection of stable chinese hamster ovary (CHO) cell lines for expression of recombinant proteins by using human interferon beta SAR element
    • Kim, J.D.; Yoon, Y.; Hwang, H.Y.; Park, J.S.; Yu, S.; Lee, J.; Baek, K.; Yoon, J. Efficient selection of stable chinese hamster ovary (CHO) cell lines for expression of recombinant proteins by using human interferon beta SAR element. Biotechnol. Prog. 2005, 21, 933-937.
    • (2005) Biotechnol. Prog , vol.21 , pp. 933-937
    • Kim, J.D.1    Yoon, Y.2    Hwang, H.Y.3    Park, J.S.4    Yu, S.5    Lee, J.6    Baek, K.7    Yoon, J.8
  • 53
    • 0036422427 scopus 로고    scopus 로고
    • The use of UCOE vectors in combination with a preadapted serum free, suspension cell line allows for rapid production of large quantities of protein
    • Benton, T.; Chen, T.; McEntee, M.; Fox, B.; King, D.; Crombie, R.; Thomas, T.C.; Bebbington, C. The use of UCOE vectors in combination with a preadapted serum free, suspension cell line allows for rapid production of large quantities of protein. Cytotechnology 2002, 38, 43-46.
    • (2002) Cytotechnology , vol.38 , pp. 43-46
    • Benton, T.1    Chen, T.2    McEntee, M.3    Fox, B.4    King, D.5    Crombie, R.6    Thomas, T.C.7    Bebbington, C.8
  • 54
    • 34548105386 scopus 로고    scopus 로고
    • Optimization of short-term transgene expression by sodium butyrate and ubiquitous chromatin opening elements (UCOEs)
    • De Poorter, J.J.; Lipinski, K.S.; Nelissen, R.G; Huizinga, T.W.; Hoeben, R.C. Optimization of short-term transgene expression by sodium butyrate and ubiquitous chromatin opening elements (UCOEs). J. Gene Med. 2007, 9, 639-648.
    • (2007) J. Gene Med , vol.9 , pp. 639-648
    • de Poorter, J.J.1    Lipinski, K.S.2    Nelissen, R.G.3    Huizinga, T.W.4    Hoeben, R.C.5
  • 56
    • 76649086996 scopus 로고    scopus 로고
    • A "GC-rich" method for mammalian gene expression: A dominant role of non-coding DNA GC content in regulation of mammalian gene expression
    • Jia, Q.; Wu, H.; Zhou, X.; Gao, J.; Zhao, W.; Aziz, J.; Wei, J.; Hou, L.; Wu, S.; Zhang Y.; et al. A "GC-rich" method for mammalian gene expression: A dominant role of non-coding DNA GC content in regulation of mammalian gene expression. Sci. China Life Sci. 2010, 53, 94-100.
    • (2010) Sci. China Life Sci , vol.53 , pp. 94-100
    • Jia, Q.1    Wu, H.2    Zhou, X.3    Gao, J.4    Zhao, W.5    Aziz, J.6    Wei, J.7    Hou, L.8    Wu, S.9    Zhang, Y.10
  • 58
    • 0032512794 scopus 로고    scopus 로고
    • New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning
    • Lowary, P.T.; Widom, J. New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning. J. Mol. Biol. 1998, 276, 19-42.
    • (1998) J. Mol. Biol , vol.276 , pp. 19-42
    • Lowary, P.T.1    Widom, J.2
  • 59
    • 3242876859 scopus 로고    scopus 로고
    • RECON: A program for prediction of nucleosome formation potential
    • Levitsky, V.G RECON: A program for prediction of nucleosome formation potential. Nucleic Acids Res. 2004, 32, W346-W349.
    • (2004) Nucleic Acids Res , vol.32
    • Levitsky, V.G.1
  • 60
    • 34147179033 scopus 로고    scopus 로고
    • An efficient and targeted gene integration system for high-level antibody expression
    • Huang, Y.; Li, Y.; Wang, Y.G.; Gu, X.; Wang, Y.; Shen, B.F. An efficient and targeted gene integration system for high-level antibody expression. J. Immunol. Methods 2007, 322, 28-39.
    • (2007) J. Immunol. Methods , vol.322 , pp. 28-39
    • Huang, Y.1    Li, Y.2    Wang, Y.G.3    Gu, X.4    Wang, Y.5    Shen, B.F.6
  • 61
    • 0027920028 scopus 로고
    • Genetics. Small and perfectly formed
    • Little, P. Genetics. Small and perfectly formed. Nature 1993, 366, 204-205.
    • (1993) Nature , vol.366 , pp. 204-205
    • Little, P.1
  • 62
    • 0346503889 scopus 로고    scopus 로고
    • Talking about a revolution: The impact of site-specific recombinases on genetic analyses in mice
    • Branda, C.S.; Dymecki, S.M. Talking about a revolution: The impact of site-specific recombinases on genetic analyses in mice. Dev. Cell 2004, 6, 7-28.
    • (2004) Dev. Cell , vol.6 , pp. 7-28
    • Branda, C.S.1    Dymecki, S.M.2
  • 63
    • 2442458847 scopus 로고    scopus 로고
    • Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31
    • Groth, A.C.; Fish, M.; Nusse, R.; Calos, M.P. Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31. Genetics 2004, 166, 1775-1782.
    • (2004) Genetics , vol.166 , pp. 1775-1782
    • Groth, A.C.1    Fish, M.2    Nusse, R.3    Calos, M.P.4
  • 64
    • 0030880076 scopus 로고    scopus 로고
    • FLP-mediated DNA mobilization to specific target sites in Drosophila chromosomes
    • Golic, M.M.; Rong, Y.S.; Petersen, R.B.; Lindquist, S.L.; Golic, K.G. FLP-mediated DNA mobilization to specific target sites in Drosophila chromosomes. Nucleic Acids Res. 1997, 25, 3665-3671.
    • (1997) Nucleic Acids Res , vol.25 , pp. 3665-3671
    • Golic, M.M.1    Rong, Y.S.2    Petersen, R.B.3    Lindquist, S.L.4    Golic, K.G.5
  • 65
    • 0033557147 scopus 로고    scopus 로고
    • A general model for site-specific recombination by the integrase family recombinases
    • Voziyanov, Y.; Pathania, S.; Jayaram, M. A general model for site-specific recombination by the integrase family recombinases. Nucleic Acids Res. 1999, 27, 930-941.
    • (1999) Nucleic Acids Res , vol.27 , pp. 930-941
    • Voziyanov, Y.1    Pathania, S.2    Jayaram, M.3
  • 67
    • 0025847620 scopus 로고
    • Recombinase-mediated gene activation and site-specific integration in mammalian cells
    • O'Gorman, S.; Fox, D.T.; Wahl, G.M. Recombinase-mediated gene activation and site-specific integration in mammalian cells. Science 1991, 251, 1351-1355.
    • (1991) Science , vol.251 , pp. 1351-1355
    • O'Gorman, S.1    Fox, D.T.2    Wahl, G.M.3
  • 68
    • 0037423742 scopus 로고    scopus 로고
    • Stepwise manipulation of DNA specificity in Flp recombinase: Progressively adapting Flp to individual and combinatorial mutations in its target site
    • Voziyanov, Y.; Konieczka, J.H.; Stewart, A.F.; Jayaram, M. Stepwise manipulation of DNA specificity in Flp recombinase: Progressively adapting Flp to individual and combinatorial mutations in its target site. J. Mol. Biol. 2003, 326, 65-76.
    • (2003) J. Mol. Biol , vol.326 , pp. 65-76
    • Voziyanov, Y.1    Konieczka, J.H.2    Stewart, A.F.3    Jayaram, M.4
  • 69
    • 0037212982 scopus 로고    scopus 로고
    • Construction of engineered CHO strains for high-level production of recombinant proteins
    • Kito, M.; Itami, S.; Fukano, Y.; Yamana, K.; Shibui, T. Construction of engineered CHO strains for high-level production of recombinant proteins. Appl. Microbiol. Biotechnol. 2002, 60, 442-448.
    • (2002) Appl. Microbiol. Biotechnol , vol.60 , pp. 442-448
    • Kito, M.1    Itami, S.2    Fukano, Y.3    Yamana, K.4    Shibui, T.5
  • 70
    • 77951552865 scopus 로고    scopus 로고
    • An accumulative site-specific gene integration system using Cre recombinase-mediated cassette exchange
    • Kameyama, Y.; Kawabe, Y.; Ito, A.; Kamihira, M. An accumulative site-specific gene integration system using Cre recombinase-mediated cassette exchange. Biotechnol. Bioeng. 2010, 105, 1106-1114.
    • (2010) Biotechnol. Bioeng , vol.105 , pp. 1106-1114
    • Kameyama, Y.1    Kawabe, Y.2    Ito, A.3    Kamihira, M.4
  • 71
    • 0036229438 scopus 로고    scopus 로고
    • Diversity in the serine recombinases
    • Smith, M.C.; Thorpe H.M. Diversity in the serine recombinases. Mol. Microbiol. 2002, 44, 299-307.
    • (2002) Mol. Microbiol , vol.44 , pp. 299-307
    • Smith, M.C.1    Thorpe, H.M.2
  • 72
    • 33646843556 scopus 로고    scopus 로고
    • Phage Bxb1 integrase mediates highly efficient site-specific recombination in mammalian cells
    • Russell, J.P.; Chang, D.W.; Tretiakova, A.; Padidam, M. Phage Bxb1 integrase mediates highly efficient site-specific recombination in mammalian cells. Biotechniques 2006, 40, 460, 462-464.
    • (2006) Biotechniques , vol.40 , Issue.460 , pp. 462-464
    • Russell, J.P.1    Chang, D.W.2    Tretiakova, A.3    Padidam, M.4
  • 73
    • 77954928400 scopus 로고    scopus 로고
    • Utilization of site-specific recombination for generating therapeutic protein producing cell lines
    • Campbell, M.; Corisdeo, S.; McGee, C.; Kraichely, D. Utilization of site-specific recombination for generating therapeutic protein producing cell lines. Mol. Biotechnol. 2010, 45, 199-202.
    • (2010) Mol. Biotechnol , vol.45 , pp. 199-202
    • Campbell, M.1    Corisdeo, S.2    McGee, C.3    Kraichely, D.4
  • 74
    • 79960924581 scopus 로고    scopus 로고
    • Engineered mammalian chromosomes in cellular protein production: Future prospects
    • Kennard, M.L. Engineered mammalian chromosomes in cellular protein production: Future prospects. Methods Mol. Biol. 2011, 738, 217-238.
    • (2011) Methods Mol. Biol , vol.738 , pp. 217-238
    • Kennard, M.L.1
  • 75
    • 70349329466 scopus 로고    scopus 로고
    • The generation of stable, high MAb expressing CHO cell lines based on the artificial chromosome expression (ACE) technology
    • Kennard, M.L.; Goosney, D.L.; Monteith, D.; Zhang, L.; Moffat, M.; Fischer, D.; Mott, J. The generation of stable, high MAb expressing CHO cell lines based on the artificial chromosome expression (ACE) technology. Biotechnol. Bioeng. 2009, 104, 540-553.
    • (2009) Biotechnol. Bioeng , vol.104 , pp. 540-553
    • Kennard, M.L.1    Goosney, D.L.2    Monteith, D.3    Zhang, L.4    Moffat, M.5    Fischer, D.6    Mott, J.7
  • 76
    • 0033081317 scopus 로고    scopus 로고
    • Mammalian artificial chromosome pilot production facility: Large-scale isolation of functional satellite DNA-based artificial chromosomes
    • Dejong, G.; Telenius, A.H.; Telenius, H.; Perez, C.F.; Drayer, J.I.; Hadlaczky, G. Mammalian artificial chromosome pilot production facility: Large-scale isolation of functional satellite DNA-based artificial chromosomes. Cytometry 1999, 35, 129-133.
    • (1999) Cytometry , vol.35 , pp. 129-133
    • Dejong, G.1    Telenius, A.H.2    Telenius, H.3    Perez, C.F.4    Drayer, J.I.5    Hadlaczky, G.6
  • 78
    • 57349087717 scopus 로고    scopus 로고
    • Effect of Akt overexpression on programmed cell death in antibody-producing Chinese hamster ovary cells
    • Hwang, S.O.; Lee, G.M. Effect of Akt overexpression on programmed cell death in antibody-producing Chinese hamster ovary cells. J. Biotechnol. 2009, 139, 89-94.
    • (2009) J. Biotechnol , vol.139 , pp. 89-94
    • Hwang, S.O.1    Lee, G.M.2
  • 79
    • 79952581899 scopus 로고    scopus 로고
    • Ectopic expression of human mTOR increases viability, robustness, cell size, proliferation, and antibody production of chinese hamster ovary cells
    • Dreesen, I.A.; Fussenegger, M. Ectopic expression of human mTOR increases viability, robustness, cell size, proliferation, and antibody production of chinese hamster ovary cells. Biotechnol. Bioeng. 2011, 108, 853-866.
    • (2011) Biotechnol. Bioeng , vol.108 , pp. 853-866
    • Dreesen, I.A.1    Fussenegger, M.2
  • 80
    • 39149105287 scopus 로고    scopus 로고
    • The role of Bcl-2 and its combined effect with p21CIP1 in adaptation of CHO cells to suspension and protein-free culture
    • Astley, K.; Al-Rubeai, M. The role of Bcl-2 and its combined effect with p21CIP1 in adaptation of CHO cells to suspension and protein-free culture. Appl. Microbiol. Biotechnol. 2008, 78, 391-399.
    • (2008) Appl. Microbiol. Biotechnol , vol.78 , pp. 391-399
    • Astley, K.1    Al-Rubeai, M.2
  • 81
    • 79955540196 scopus 로고    scopus 로고
    • Decreasing lactate level and increasing antibody production in Chinese Hamster Ovary cells (CHO) by reducing the expression of lactate dehydrogenase and pyruvate dehydrogenase kinases
    • Zhou, M.; Crawford, Y.; Ng, D.; Tung, J.; Pynn, A.F.; Meier, A.; Yuk, I.H.; Vijayasankaran, N.; Leach, K.; Joly J.; et al. Decreasing lactate level and increasing antibody production in Chinese Hamster Ovary cells (CHO) by reducing the expression of lactate dehydrogenase and pyruvate dehydrogenase kinases. J. Biotechnol. 2011, 153, 27-34.
    • (2011) J. Biotechnol , vol.153 , pp. 27-34
    • Zhou, M.1    Crawford, Y.2    Ng, D.3    Tung, J.4    Pynn, A.F.5    Meier, A.6    Yuk, I.H.7    Vijayasankaran, N.8    Leach, K.9    Joly, J.10
  • 82
    • 78751507365 scopus 로고    scopus 로고
    • Differential effect of exocytic SNAREs on the production of recombinant proteins in mammalian cells
    • Peng, R.W.; Abellan, E.; Fussenegger, M. Differential effect of exocytic SNAREs on the production of recombinant proteins in mammalian cells. Biotechnol. Bioeng. 2011, 108, 611-620.
    • (2011) Biotechnol. Bioeng , vol.108 , pp. 611-620
    • Peng, R.W.1    Abellan, E.2    Fussenegger, M.3
  • 83
  • 84
    • 44949158950 scopus 로고    scopus 로고
    • Assessment of cell engineering strategies for improved therapeutic protein production in CHO cells
    • Mohan, C.; Kim, Y.G.; Koo, J.; Lee, G.M. Assessment of cell engineering strategies for improved therapeutic protein production in CHO cells. Biotechnol. J. 2008, 3, 624-630.
    • (2008) Biotechnol. J , vol.3 , pp. 624-630
    • Mohan, C.1    Kim, Y.G.2    Koo, J.3    Lee, G.M.4
  • 85
    • 77950461287 scopus 로고    scopus 로고
    • Evaluation of a combinatorial cell engineering approach to overcome apoptotic effects in XBP-1(s) expressing cells
    • Becker, E.; Florin, L.; Pfizenmaier, K.; Kaufmann, H. Evaluation of a combinatorial cell engineering approach to overcome apoptotic effects in XBP-1(s) expressing cells. J. Biotechnol. 2010, 146, 198-206.
    • (2010) J. Biotechnol , vol.146 , pp. 198-206
    • Becker, E.1    Florin, L.2    Pfizenmaier, K.3    Kaufmann, H.4
  • 86
    • 3142658576 scopus 로고    scopus 로고
    • XBP1, downstream of Blimp-1, expands the secretory apparatus and other organelles, and increases protein synthesis in plasma cell differentiation
    • Shaffer, A.L.; Shapiro-Shelef, M.; Iwakoshi, N.N.; Lee, A.H.; Qian, S.B.; Zhao, H.; Yu, X.; Yang, L.; Tan, B.K.; Rosenwald A, et al. XBP1, downstream of Blimp-1, expands the secretory apparatus and other organelles, and increases protein synthesis in plasma cell differentiation. Immunity 2004, 21, 81-93.
    • (2004) Immunity , vol.21 , pp. 81-93
    • Shaffer, A.L.1    Shapiro-Shelef, M.2    Iwakoshi, N.N.3    Lee, A.H.4    Qian, S.B.5    Zhao, H.6    Yu, X.7    Yang, L.8    Tan, B.K.9    Rosenwald, A.10
  • 88
    • 84865513069 scopus 로고    scopus 로고
    • TALE nucleases: Tailored genome engineering made easy
    • Mussolino, C.; Cathomen, T. TALE nucleases: Tailored genome engineering made easy. Curr. Opin. Biotechnol. 2012, 23, 644-650.
    • (2012) Curr. Opin. Biotechnol , vol.23 , pp. 644-650
    • Mussolino, C.1    Cathomen, T.2
  • 89
    • 72149090954 scopus 로고    scopus 로고
    • A simple cipher governs DNA recognition by TAL effectors
    • Moscou, M.J.; Bogdanove, A.J. A simple cipher governs DNA recognition by TAL effectors. Science 2009, 326, 1501.
    • (2009) Science , vol.326 , pp. 1501
    • Moscou, M.J.1    Bogdanove, A.J.2
  • 92
    • 84857444298 scopus 로고    scopus 로고
    • Improving the efficiency of CHO cell line generation using glutamine synthetase gene knockout cells
    • Fan, L.; Kadura, I.; Krebs, L.E.; Hatfield, C.C.; Shaw, M.M.; Frye, C.C. Improving the efficiency of CHO cell line generation using glutamine synthetase gene knockout cells. Biotechnol. Bioeng. 2012, 109, 1007-1015.
    • (2012) Biotechnol. Bioeng , vol.109 , pp. 1007-1015
    • Fan, L.1    Kadura, I.2    Krebs, L.E.3    Hatfield, C.C.4    Shaw, M.M.5    Frye, C.C.6
  • 93
    • 0035883706 scopus 로고    scopus 로고
    • Homing endonucleases: Structural and functional insight into the catalysts of intron/intein mobility
    • Chevalier, B.S.; Stoddard, B.L. Homing endonucleases: Structural and functional insight into the catalysts of intron/intein mobility. Nucleic Acids Res. 2001, 29, 3757-3774.
    • (2001) Nucleic Acids Res , vol.29 , pp. 3757-3774
    • Chevalier, B.S.1    Stoddard, B.L.2
  • 96
    • 67651209209 scopus 로고    scopus 로고
    • On the reconstruction of the Mus musculus genome-scale metabolic network model
    • Quek, L.E.; Nielsen, L.K. On the reconstruction of the Mus musculus genome-scale metabolic network model. Genome Inform. 2008, 21, 89-100.
    • (2008) Genome Inform , vol.21 , pp. 89-100
    • Quek, L.E.1    Nielsen, L.K.2
  • 97
    • 0033735527 scopus 로고    scopus 로고
    • The gel microdrop secretion assay: Identification of a low productivity subpopulation arising during the production of human antibody in CHO cells
    • Hammill, L.; Welles, J.; Carson, G.R. The gel microdrop secretion assay: Identification of a low productivity subpopulation arising during the production of human antibody in CHO cells. Cytotechnology 2000, 34, 27-37.
    • (2000) Cytotechnology , vol.34 , pp. 27-37
    • Hammill, L.1    Welles, J.2    Carson, G.R.3
  • 98
    • 0023838813 scopus 로고
    • Hazards of the limiting-dilution method of cloning hybridomas
    • Underwood, P.A.; Bean, P.A. Hazards of the limiting-dilution method of cloning hybridomas. J. Immunol. Methods 1988, 107, 119-128.
    • (1988) J. Immunol. Methods , vol.107 , pp. 119-128
    • Underwood, P.A.1    Bean, P.A.2
  • 99
    • 73149119826 scopus 로고    scopus 로고
    • Ultrahigh-throughput FACS-based screening for directed enzyme evolution
    • Yang, G.; Withers, S.G. Ultrahigh-throughput FACS-based screening for directed enzyme evolution. Chembiochem 2009, 10, 2704-2715.
    • (2009) Chembiochem , vol.10 , pp. 2704-2715
    • Yang, G.1    Withers, S.G.2
  • 101
    • 0033969002 scopus 로고    scopus 로고
    • Green fluorescent protein as a second selectable marker for selection of high producing clones from transfected CHO cells
    • Meng, Y.G.; Liang, J.; Wong, W.L.; Chisholm, V. Green fluorescent protein as a second selectable marker for selection of high producing clones from transfected CHO cells. Gene 2000, 242, 201-207.
    • (2000) Gene , vol.242 , pp. 201-207
    • Meng, Y.G.1    Liang, J.2    Wong, W.L.3    Chisholm, V.4
  • 102
    • 0035812441 scopus 로고    scopus 로고
    • Flow cytometry: An improved method for the selection of highly productive gene-amplified CHO cells using flow cytometry
    • Yoshikawa, T.; Nakanishi, F.; Ogura, Y.; Oi, D.; Omasa, T.; Katakura, Y.; Kishimoto, M.; Suga, K.I. Flow cytometry: An improved method for the selection of highly productive gene-amplified CHO cells using flow cytometry. Biotechnol. Bioeng. 2001, 74, 435-442.
    • (2001) Biotechnol. Bioeng , vol.74 , pp. 435-442
    • Yoshikawa, T.1    Nakanishi, F.2    Ogura, Y.3    Oi, D.4    Omasa, T.5    Katakura, Y.6    Kishimoto, M.7    Suga, K.I.8
  • 103
    • 3242712345 scopus 로고    scopus 로고
    • Comparison of results using the gel microdrop cytokine secretion assay with ELISPOT and intracellular cytokine staining assay
    • Atochina, O.; Mylvaganam, R.; Akselband, Y.; McGrath, P. Comparison of results using the gel microdrop cytokine secretion assay with ELISPOT and intracellular cytokine staining assay. Cytokine 2004, 27, 120-128.
    • (2004) Cytokine , vol.27 , pp. 120-128
    • Atochina, O.1    Mylvaganam, R.2    Akselband, Y.3    McGrath, P.4
  • 104
    • 0029029084 scopus 로고
    • Secretion capture and report web: Use of affinity derivatized agarose microdroplets for the selection of hybridoma cells
    • Gray, F.; Kenney, J.S.; Dunne, J.F. Secretion capture and report web: Use of affinity derivatized agarose microdroplets for the selection of hybridoma cells. J. Immunol. Methods 1995, 182, 155-163.
    • (1995) J. Immunol. Methods , vol.182 , pp. 155-163
    • Gray, F.1    Kenney, J.S.2    Dunne, J.F.3
  • 105
    • 11944254485 scopus 로고
    • Gel microdroplets and flow cytometry: Rapid determination of antibody secretion by individual cells within a cell population
    • Powell, K.T.; Weaver, J.C. Gel microdroplets and flow cytometry: Rapid determination of antibody secretion by individual cells within a cell population. Biotechnology (NY) 1990, 8, 333-337.
    • (1990) Biotechnology (NY) , vol.8 , pp. 333-337
    • Powell, K.T.1    Weaver, J.C.2
  • 106
    • 0028960201 scopus 로고
    • Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix
    • Manz, R.; Assenmacher, M.; Pfluger, E.; Miltenyi, S.; Radbruch, A. Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix. Proc. Natl. Acad. Sci. USA 1995, 92, 1921-1925.
    • (1995) Proc. Natl. Acad. Sci. USA , vol.92 , pp. 1921-1925
    • Manz, R.1    Assenmacher, M.2    Pfluger, E.3    Miltenyi, S.4    Radbruch, A.5
  • 107
    • 0032758728 scopus 로고    scopus 로고
    • Improved cell line development by a high throughput affinity capture surface display technique to select for high secretors
    • Holmes, P.; Al-Rubeai, M. Improved cell line development by a high throughput affinity capture surface display technique to select for high secretors. J. Immunol. Methods 1999, 230, 141-147.
    • (1999) J. Immunol. Methods , vol.230 , pp. 141-147
    • Holmes, P.1    Al-Rubeai, M.2
  • 109
    • 36348949333 scopus 로고    scopus 로고
    • High-throughput screening of cell lines expressing monoclonal antibodies
    • Lee, C.; Ly, C.; Sauerwald, T.; Kelly, T.; Moore, G. High-throughput screening of cell lines expressing monoclonal antibodies. BioProcess Int. 2006, 4, 32-35.
    • (2006) BioProcess Int , vol.4 , pp. 32-35
    • Lee, C.1    Ly, C.2    Sauerwald, T.3    Kelly, T.4    Moore, G.5
  • 110
    • 79955493477 scopus 로고    scopus 로고
    • Rapid automated selection of mammalian cell line secreting high level of humanized monoclonal antibody using Clone Pix FL system and the correlation between exterior median intensity and antibody productivity
    • doi:10.2225/vol14-issue2-fulltext-7
    • Dharshanan, S.; Chong, H.; Hung, C.S.; Zamrod, Z.; Kamal, N. Rapid automated selection of mammalian cell line secreting high level of humanized monoclonal antibody using Clone Pix FL system and the correlation between exterior median intensity and antibody productivity. Electron. J. Biotechnol. 2011, 14, doi:10.2225/vol14-issue2-fulltext-7.
    • (2011) Electron. J. Biotechnol , vol.14
    • Dharshanan, S.1    Chong, H.2    Hung, C.S.3    Zamrod, Z.4    Kamal, N.5
  • 113
    • 79956298130 scopus 로고    scopus 로고
    • Murine insulin growth factor-like (IGFL) and human IGFL1 proteins are induced in inflammatory skin conditions and bind to a novel tumor necrosis factor receptor family member, IGFLR1
    • Lobito, A.A.; Ramani, S.R.; Tom, I.; Bazan, J.F.; Luis, E.; Fairbrother, W.J.; Ouyang, W.; Gonzalez, L.C. Murine insulin growth factor-like (IGFL) and human IGFL1 proteins are induced in inflammatory skin conditions and bind to a novel tumor necrosis factor receptor family member, IGFLR1. J. Biol. Chem. 2011, 286, 18969-18981.
    • (2011) J. Biol. Chem , vol.286 , pp. 18969-18981
    • Lobito, A.A.1    Ramani, S.R.2    Tom, I.3    Bazan, J.F.4    Luis, E.5    Fairbrother, W.J.6    Ouyang, W.7    Gonzalez, L.C.8
  • 114
    • 25144433398 scopus 로고    scopus 로고
    • Automated in situ measurement of cell-specific antibody secretion and laser-mediated purification for rapid cloning of highly-secreting producers
    • Hanania, E.G.; Fieck, A.; Stevens, J.; Bodzin, L.J.; Palsson, B.O.; Koller, M.R. Automated in situ measurement of cell-specific antibody secretion and laser-mediated purification for rapid cloning of highly-secreting producers. Biotechnol. Bioeng. 2005, 91, 872-876.
    • (2005) Biotechnol. Bioeng , vol.91 , pp. 872-876
    • Hanania, E.G.1    Fieck, A.2    Stevens, J.3    Bodzin, L.J.4    Palsson, B.O.5    Koller, M.R.6
  • 116
    • 84876989287 scopus 로고    scopus 로고
    • Cell Xpress™ Technology Facilitates High-Producing Chinese Hamster Ovary Cell Line Generation Using Glutamine Synthetase Gene Expression System
    • Noll, T., Ed. Springer: Berlin, Heidelberg, Germany
    • Richardson, G.; Lin, N.; Lacy, K.; Davis, L.; Gray, M.; Cresswell, J.; Gerber, M.; Caple, M.; Kayser, K. Cell Xpress™ Technology Facilitates High-Producing Chinese Hamster Ovary Cell Line Generation Using Glutamine Synthetase Gene Expression System. In Cells and Culture; Noll, T., Ed. Springer: Berlin, Heidelberg, Germany, 2010; Volume 4, pp. 45-48.
    • (2010) Cells and Culture , vol.4 , pp. 45-48
    • Richardson, G.1    Lin, N.2    Lacy, K.3    Davis, L.4    Gray, M.5    Cresswell, J.6    Gerber, M.7    Caple, M.8    Kayser, K.9
  • 118
    • 78650967062 scopus 로고    scopus 로고
    • New strategy for large-scale preparation of the extracellular domain of tumor-associated antigen HAb18G/CD147 (HAb18GED)
    • Zhang, Z.; Yang, X.; Yang, H.; Yu, X.; Li, Y.; Xing, J.; Chen, Z. New strategy for large-scale preparation of the extracellular domain of tumor-associated antigen HAb18G/CD147 (HAb18GED). J. Biosci. Bioeng. 2011, 111, 1-6.
    • (2011) J. Biosci. Bioeng , vol.111 , pp. 1-6
    • Zhang, Z.1    Yang, X.2    Yang, H.3    Yu, X.4    Li, Y.5    Xing, J.6    Chen, Z.7
  • 122
    • 33645573249 scopus 로고    scopus 로고
    • Initial transcriptome and proteome analyses of low culture temperature-induced expression in CHO cells producing erythropoietin
    • Baik, J.Y.; Lee, M.S.; An, S.R.; Yoon, S.K.; Joo, E.J.; Kim, Y.H.; Park, H.W.; Lee, G.M. Initial transcriptome and proteome analyses of low culture temperature-induced expression in CHO cells producing erythropoietin. Biotechnol. Bioeng. 2006, 93, 361-371.
    • (2006) Biotechnol. Bioeng , vol.93 , pp. 361-371
    • Baik, J.Y.1    Lee, M.S.2    An, S.R.3    Yoon, S.K.4    Joo, E.J.5    Kim, Y.H.6    Park, H.W.7    Lee, G.M.8
  • 123
    • 33846839360 scopus 로고    scopus 로고
    • Comparative transcriptional analysis of mouse hybridoma and recombinant Chinese hamster ovary cells undergoing butyrate treatment
    • De Leon Gatti, M.; Wlaschin, K.F.; Nissom, P.M.; Yap, M.; Hu, W.S. Comparative transcriptional analysis of mouse hybridoma and recombinant Chinese hamster ovary cells undergoing butyrate treatment. J. Biosci. Bioeng. 2007, 103, 82-91.
    • (2007) J. Biosci. Bioeng , vol.103 , pp. 82-91
    • De Leon Gatti, M.1    Wlaschin, K.F.2    Nissom, P.M.3    Yap, M.4    Hu, W.S.5
  • 129
    • 84862778524 scopus 로고    scopus 로고
    • Profiling conserved microRNA expression in recombinant CHO cell lines using Illumina sequencing
    • Hammond, S.; Swanberg, J.C.; Polson, S.W.; Lee, K.H. Profiling conserved microRNA expression in recombinant CHO cell lines using Illumina sequencing. Biotechnol. Bioeng. 2012, 109, 1371-1375.
    • (2012) Biotechnol. Bioeng , vol.109 , pp. 1371-1375
    • Hammond, S.1    Swanberg, J.C.2    Polson, S.W.3    Lee, K.H.4
  • 131
    • 84859926395 scopus 로고    scopus 로고
    • A screening method to assess biological effects of microRNA overexpression in Chinese hamster ovary cells
    • Jadhav, V.; Hackl, M.; Bort, J.A.; Wieser, M.; Harreither, E.; Kunert, R.; Borth, N.; Grillari, J. A screening method to assess biological effects of microRNA overexpression in Chinese hamster ovary cells. Biotechnol. Bioeng. 2012, 109, 1376-1385.
    • (2012) Biotechnol. Bioeng , vol.109 , pp. 1376-1385
    • Jadhav, V.1    Hackl, M.2    Bort, J.A.3    Wieser, M.4    Harreither, E.5    Kunert, R.6    Borth, N.7    Grillari, J.8
  • 132
    • 79955132774 scopus 로고    scopus 로고
    • Next-generation sequencing of the Chinese hamster ovary microRNA transcriptome: Identification, annotation and profiling of microRNAs as targets for cellular engineering
    • Hackl, M.; Jakobi, T.; Blom, J.; Doppmeier, D.; Brinkrolf, K.; Szczepanowski, R.; Bernhart, S.H.; Honer Zu Siederdissen, C.; Bort, J.A.; Wieser M, et al. Next-generation sequencing of the Chinese hamster ovary microRNA transcriptome: Identification, annotation and profiling of microRNAs as targets for cellular engineering. J. Biotechnol. 2011, 153, 62-75.
    • (2011) J. Biotechnol , vol.153 , pp. 62-75
    • Hackl, M.1    Jakobi, T.2    Blom, J.3    Doppmeier, D.4    Brinkrolf, K.5    Szczepanowski, R.6    Bernhart, S.H.7    Honer Zu Siederdissen, C.8    Bort, J.A.9    Wieser, M.10
  • 133
    • 45049085323 scopus 로고    scopus 로고
    • MicroRNAs as targets for engineering of CHO cell factories
    • Muller, D.; Katinger, H.; Grillari, J. MicroRNAs as targets for engineering of CHO cell factories. Trends Biotechnol. 2008, 26, 359-365.
    • (2008) Trends Biotechnol , vol.26 , pp. 359-365
    • Muller, D.1    Katinger, H.2    Grillari, J.3
  • 134
    • 0033044588 scopus 로고    scopus 로고
    • Engineered glycoforms of an antineuroblastoma IgG1 with optimized antibody-dependent cellular cytotoxic activity
    • Umana, P.; Jean-Mairet, J.; Moudry, R.; Amstutz, H.; Bailey, J.E. Engineered glycoforms of an antineuroblastoma IgG1 with optimized antibody-dependent cellular cytotoxic activity. Nat. Biotechnol. 1999, 17, 176-180.
    • (1999) Nat. Biotechnol , vol.17 , pp. 176-180
    • Umana, P.1    Jean-Mairet, J.2    Moudry, R.3    Amstutz, H.4    Bailey, J.E.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.