메뉴 건너뛰기




Volumn 78, Issue 3, 2008, Pages 391-399

The role of Bcl-2 and its combined effect with p21CIP1 in adaptation of CHO cells to suspension and protein-free culture

Author keywords

Apoptosis; Bcl 2; Cell culture; Cell cycle; p21; Proliferation

Indexed keywords

CELL CULTURE; CELL PROLIFERATION; GENE EXPRESSION; PROTEINS; SUSPENSIONS (FLUIDS);

EID: 39149105287     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-007-1320-2     Document Type: Article
Times cited : (19)

References (42)
  • 1
    • 0025500378 scopus 로고
    • Mechanisms and kinetics of monoclonal antibody synthesis and secretion in synchronous and asynchronous hybridoma cell cultures
    • Al-Rubeai M, Emery AN (1990) Mechanisms and kinetics of monoclonal antibody synthesis and secretion in synchronous and asynchronous hybridoma cell cultures. J Biotechnol 16:67-86
    • (1990) J Biotechnol , vol.16 , pp. 67-86
    • Al-Rubeai, M.1    Emery, A.N.2
  • 2
    • 0027024341 scopus 로고
    • Specific antibody productivity and the cell cycle comparisons of batch, continuous and perfusion cultures
    • Al-Rubeai M, Emery AN, Chalder S, Jan DC (1992a) Specific antibody productivity and the cell cycle comparisons of batch, continuous and perfusion cultures. Cytotechnology 9:85-87
    • (1992) Cytotechnology , vol.9 , pp. 85-87
    • Al-Rubeai, M.1    Emery, A.N.2    Chalder, S.3    Jan, D.C.4
  • 3
    • 0026575910 scopus 로고
    • The effect of Pluronic F-68 on hybridoma cells in continuous culture
    • Al-Rubeai M, Emery AN, Chalder S (1992b) The effect of Pluronic F-68 on hybridoma cells in continuous culture. Appl Microbiol Biotechnol 37:44-45
    • (1992) Appl Microbiol Biotechnol , vol.37 , pp. 44-45
    • Al-Rubeai, M.1    Emery, A.N.2    Chalder, S.3
  • 4
    • 0029636268 scopus 로고
    • Cell cycle and cell size dependence of susceptibility to hydrodynamic forces
    • Al-Rubeai M, Singh RP, Emery AN, Zhang Z (1995) Cell cycle and cell size dependence of susceptibility to hydrodynamic forces. Biotechnol Bioeng 46:88-92
    • (1995) Biotechnol Bioeng , vol.46 , pp. 88-92
    • Al-Rubeai, M.1    Singh, R.P.2    Emery, A.N.3    Zhang, Z.4
  • 5
    • 0028485585 scopus 로고
    • Hybridoma growth and monoclonal antibody production in a dialysis prefusion system
    • Amos B, Al-Rubeai M, Emery AN (1994) Hybridoma growth and monoclonal antibody production in a dialysis prefusion system. Enzyme Microb Technol 16:688-695
    • (1994) Enzyme Microb Technol , vol.16 , pp. 688-695
    • Amos, B.1    Al-Rubeai, M.2    Emery, A.N.3
  • 6
  • 7
    • 1542410260 scopus 로고    scopus 로고
    • Uncoupling of cell growth and proliferation results in enhancement of productivity in p21 arrested CHO cells
    • Bi J-X, Shuttleworth J, Al-Rubeai M (2004) Uncoupling of cell growth and proliferation results in enhancement of productivity in p21 arrested CHO cells. Biotechnol Bioeng 85:741-749
    • (2004) Biotechnol Bioeng , vol.85 , pp. 741-749
    • Bi, J.-X.1    Shuttleworth, J.2    Al-Rubeai, M.3
  • 9
    • 0032483461 scopus 로고    scopus 로고
    • Ectopic p21(WAF1) expression induces differentiation-specific cell cycle changes in PC12 cells characteristic of nerve growth factor treatment
    • Erhardt JA, Pittman RN (1998) Ectopic p21(WAF1) expression induces differentiation-specific cell cycle changes in PC12 cells characteristic of nerve growth factor treatment. J Biol Chem 273:23517-23523
    • (1998) J Biol Chem , vol.273 , pp. 23517-23523
    • Erhardt, J.A.1    Pittman, R.N.2
  • 11
    • 0031911956 scopus 로고    scopus 로고
    • Long term cultivation of immortalised mouse hepatocytes in high cell density, fixed-bed reactor
    • Fassnacht D, Rossing S, Strange J, Porter R (1998a) Long term cultivation of immortalised mouse hepatocytes in high cell density, fixed-bed reactor. Biotechnol Techniq 12:25-30
    • (1998) Biotechnol Techniq , vol.12 , pp. 25-30
    • Fassnacht, D.1    Rossing, S.2    Strange, J.3    Porter, R.4
  • 12
    • 0031899540 scopus 로고    scopus 로고
    • Effect of Bcl-2 expression on hybridoma cell growth in serum supplemented, protein free and diluted media
    • Fassnacht D, Rossing S, Franek F, Al-Rubeai M, Porter R (1998b) Effect of Bcl-2 expression on hybridoma cell growth in serum supplemented, protein free and diluted media. Cytotechnol 26:219-225
    • (1998) Cytotechnol , vol.26 , pp. 219-225
    • Fassnacht, D.1    Rossing, S.2    Franek, F.3    Al-Rubeai, M.4    Porter, R.5
  • 13
    • 0025756428 scopus 로고
    • Nucleosomes occurring in protein free hybridoma cell cultures. Evidence for programmed cell death
    • Franek F, Dolnikova J (1991) Nucleosomes occurring in protein free hybridoma cell cultures. Evidence for programmed cell death. FEBS Lett 248:285-287
    • (1991) FEBS Lett , vol.248 , pp. 285-287
    • Franek, F.1    Dolnikova, J.2
  • 14
    • 0014661176 scopus 로고
    • Spin filter culture: The propagation of mammalian cells in suspension
    • Himmelfarb P, Thayer PS, Martin HE (1969) Spin filter culture: the propagation of mammalian cells in suspension. Science 164:555-557
    • (1969) Science , vol.164 , pp. 555-557
    • Himmelfarb, P.1    Thayer, P.S.2    Martin, H.E.3
  • 16
    • 0032726415 scopus 로고    scopus 로고
    • Role of Ca, Mg and K ions in determining apoptosis and extent of suppression afforded by bcl-2 during hybridoma cell culture
    • Ishaque A, Al-Rubeai M (1999) Role of Ca, Mg and K ions in determining apoptosis and extent of suppression afforded by bcl-2 during hybridoma cell culture. Apoptosis 4:335-355
    • (1999) Apoptosis , vol.4 , pp. 335-355
    • Ishaque, A.1    Al-Rubeai, M.2
  • 17
    • 0036016922 scopus 로고    scopus 로고
    • Role of vitamins in determining apoptosis and extent of suppression by bcl-2 during hybridoma cell culture
    • Ishaque A, Al-Rubeai M (2002) Role of vitamins in determining apoptosis and extent of suppression by bcl-2 during hybridoma cell culture. Apoptosis 7:231-239
    • (2002) Apoptosis , vol.7 , pp. 231-239
    • Ishaque, A.1    Al-Rubeai, M.2
  • 18
    • 0029048389 scopus 로고
    • Overexpression of Bcl-2, apoptosis suppressing gene: Prolonged viable culture period of hybridoma and enhanced antibody production
    • Itoh Y, Ueda H, Suzuki E (1995) Overexpression of Bcl-2, apoptosis suppressing gene: prolonged viable culture period of hybridoma and enhanced antibody production. Biotechnol Bioeng 48:118-122
    • (1995) Biotechnol Bioeng , vol.48 , pp. 118-122
    • Itoh, Y.1    Ueda, H.2    Suzuki, E.3
  • 19
    • 0001061059 scopus 로고
    • Introducing spin filter eliminates hydrodynamic damage to hybridomas in bioreactor
    • Jan DCH, Emery AN, Al-rubeai M (1993) Introducing spin filter eliminates hydrodynamic damage to hybridomas in bioreactor. Biotechnol Techniq 7:351-356
    • (1993) Biotechnol Techniq , vol.7 , pp. 351-356
    • Jan, D.C.H.1    Emery, A.N.2    Al-Rubeai, M.3
  • 20
    • 0037454718 scopus 로고    scopus 로고
    • Effect of shear stress on expression of a recombinant protein by Chinese hamster ovary cells
    • Keane JT (2003) Effect of shear stress on expression of a recombinant protein by Chinese hamster ovary cells. Biotechnol Bioeng 81:211-220
    • (2003) Biotechnol Bioeng , vol.81 , pp. 211-220
    • Keane, J.T.1
  • 21
    • 0029173973 scopus 로고
    • Adaptation of cholesterol-requiring NS0 mouse myeloma cells to high density growth in a fully defined protein-free and cholesterol-free culture medium
    • Keen MJ, Steward TW (1995) Adaptation of cholesterol-requiring NS0 mouse myeloma cells to high density growth in a fully defined protein-free and cholesterol-free culture medium. Cyotechnol 17:203-211
    • (1995) Cyotechnol , vol.17 , pp. 203-211
    • Keen, M.J.1    Steward, T.W.2
  • 22
    • 84984086633 scopus 로고
    • The cultivation of animal cells at controlled dissolved oxygen partial pressure
    • Kilburn DG, Webb FC (1968) The cultivation of animal cells at controlled dissolved oxygen partial pressure. Biotechnol Bioeng 10:801-814
    • (1968) Biotechnol Bioeng , vol.10 , pp. 801-814
    • Kilburn, D.G.1    Webb, F.C.2
  • 23
    • 0021267816 scopus 로고
    • Serum-free medium for hybridoma and parental myeloma cell cultivation: A novel composition of growth-supporting substances
    • Kovar J, Franek F (1984) Serum-free medium for hybridoma and parental myeloma cell cultivation: a novel composition of growth-supporting substances. Immunol Lett 7:339-345
    • (1984) Immunol Lett , vol.7 , pp. 339-345
    • Kovar, J.1    Franek, F.2
  • 24
    • 0026457919 scopus 로고
    • Influence of shear stress on adherent mammalian cells during division
    • Ludwig A (1992) Influence of shear stress on adherent mammalian cells during division. Biotechnol Lett 14:881-884
    • (1992) Biotechnol Lett , vol.14 , pp. 881-884
    • Ludwig, A.1
  • 25
    • 0030440856 scopus 로고    scopus 로고
    • Bcl-2 inhibits apoptosis and extends recombinant protein production in cells infected with sinbis viral vectors
    • Mastrangelo AJ, Hardwick JM, Betenbaugh MJ (1996) Bcl-2 inhibits apoptosis and extends recombinant protein production in cells infected with sinbis viral vectors. Cytotechnology 22:169-178
    • (1996) Cytotechnology , vol.22 , pp. 169-178
    • Mastrangelo, A.J.1    Hardwick, J.M.2    Betenbaugh, M.J.3
  • 26
    • 0028765762 scopus 로고
    • Induction of apoptosis in nutrient deprived cultures of hybridoma and myeloma cells
    • Mercille S, Massie B (1994) Induction of apoptosis in nutrient deprived cultures of hybridoma and myeloma cells. Biotechnol Bioeng 44:1140-1154
    • (1994) Biotechnol Bioeng , vol.44 , pp. 1140-1154
    • Mercille, S.1    Massie, B.2
  • 27
    • 0343035687 scopus 로고    scopus 로고
    • Bcl-2 expression in Spodoptera frugiperda Sf9 and Trichoplusia ni BT-Tn-5B1-4 insect cell: Effect on recombinant protein expression and cell viability
    • Mitchell-Logean C, Murhammer DW (1997) Bcl-2 expression in Spodoptera frugiperda Sf9 and Trichoplusia ni BT-Tn-5B1-4 insect cell: effect on recombinant protein expression and cell viability. Biotechnol Bioeng 56:380-388
    • (1997) Biotechnol Bioeng , vol.56 , pp. 380-388
    • Mitchell-Logean, C.1    Murhammer, D.W.2
  • 30
    • 0032442810 scopus 로고    scopus 로고
    • Variable functions of bcl-2 in mediating bioreactor stress- induced apoptosis in hybridoma cells
    • Perani A, Singh RP, Chauhan R, Al-Rubeai M (1999) Variable functions of bcl-2 in mediating bioreactor stress- induced apoptosis in hybridoma cells. Cytotechnology 28:177-188
    • (1999) Cytotechnology , vol.28 , pp. 177-188
    • Perani, A.1    Singh, R.P.2    Chauhan, R.3    Al-Rubeai, M.4
  • 31
    • 0343812075 scopus 로고    scopus 로고
    • Kinetic studies on hybridoma cells immobilised in fixed-bed reactors
    • Portner R, Rossing S, Koop M, Ludemann I (1997) Kinetic studies on hybridoma cells immobilised in fixed-bed reactors. Biotechnol Bioeng 55:535-541
    • (1997) Biotechnol Bioeng , vol.55 , pp. 535-541
    • Portner, R.1    Rossing, S.2    Koop, M.3    Ludemann, I.4
  • 32
    • 0026161967 scopus 로고
    • Weaning of three hybridoma cell lines to serum free low protein medium
    • Radford K, Niloperbowo W, Reid S, Greenfield PF (1991) Weaning of three hybridoma cell lines to serum free low protein medium. Cytotechnology 6:65-78
    • (1991) Cytotechnology , vol.6 , pp. 65-78
    • Radford, K.1    Niloperbowo, W.2    Reid, S.3    Greenfield, P.F.4
  • 33
    • 0031554004 scopus 로고    scopus 로고
    • Prevention of hybridoma cell death by Bcl-2 during sub optimal culture conditions
    • Simpson NH, Milner AN, Al-Rubeai M (1997) Prevention of hybridoma cell death by Bcl-2 during sub optimal culture conditions. Biotechnol Bioeng 54:1-16
    • (1997) Biotechnol Bioeng , vol.54 , pp. 1-16
    • Simpson, N.H.1    Milner, A.N.2    Al-Rubeai, M.3
  • 34
    • 0033587794 scopus 로고    scopus 로고
    • Bcl-2 over-expression reduces growth rate and prolongs G1 phase in continuous chemostat cultures of hybridoma cells
    • Simpson NH, Singh RP, Emery AN, Al-Rubeai M (1999) Bcl-2 over-expression reduces growth rate and prolongs G1 phase in continuous chemostat cultures of hybridoma cells. Biotechnol Bioeng 64:174-186
    • (1999) Biotechnol Bioeng , vol.64 , pp. 174-186
    • Simpson, N.H.1    Singh, R.P.2    Emery, A.N.3    Al-Rubeai, M.4
  • 35
    • 0033846848 scopus 로고    scopus 로고
    • Adaptation of mammalian cells to growth in serum free media
    • Sinacore M, Drapeau D, Adamson SR (2000) Adaptation of mammalian cells to growth in serum free media. Mol Biotechnol 15:249-257
    • (2000) Mol Biotechnol , vol.15 , pp. 249-257
    • Sinacore, M.1    Drapeau, D.2    Adamson, S.R.3
  • 37
    • 21644458683 scopus 로고    scopus 로고
    • Suppression of apoptosis in perfusion culture of myeloma NS0 enhances cell growth but reduces antibody productivity
    • Tey BT, Al-Rubeai M (2004) Suppression of apoptosis in perfusion culture of myeloma NS0 enhances cell growth but reduces antibody productivity. Apoptosis 9:843-852
    • (2004) Apoptosis , vol.9 , pp. 843-852
    • Tey, B.T.1    Al-Rubeai, M.2
  • 38
    • 0034607272 scopus 로고    scopus 로고
    • Influence of Bcl-2 on cell death during the cultivation of a Chinese hamster ovary cell line expressing a chimeric antibody
    • Tey BT, Singh L, Piredda M, Al-Rubeai M (2000a) Influence of Bcl-2 on cell death during the cultivation of a Chinese hamster ovary cell line expressing a chimeric antibody. Biotechnol Bioeng 68:31-43
    • (2000) Biotechnol Bioeng , vol.68 , pp. 31-43
    • Tey, B.T.1    Singh, L.2    Piredda, M.3    Al-Rubeai, M.4
  • 39
    • 0034607272 scopus 로고    scopus 로고
    • Influence of Bcl-2 on cell death during cultivation of a Chinese Hamster Ovary cell line expressing a chimeric antibody
    • Tey BT, Singh RP, Piredda L, Piacentini M, Al-Rubeai M (2000b) Influence of Bcl-2 on cell death during cultivation of a Chinese Hamster Ovary cell line expressing a chimeric antibody. Biotechnol Bioeng 68:31-43
    • (2000) Biotechnol Bioeng , vol.68 , pp. 31-43
    • Tey, B.T.1    Singh, R.P.2    Piredda, L.3    Piacentini, M.4    Al-Rubeai, M.5
  • 40
    • 0022679115 scopus 로고
    • A radial flow hollow fiber reactor for large scale culture of mammalian cells
    • Tharakan JP, Chau PC (1986) A radial flow hollow fiber reactor for large scale culture of mammalian cells. Biotechnol Bioeng 28:329-342
    • (1986) Biotechnol Bioeng , vol.28 , pp. 329-342
    • Tharakan, J.P.1    Chau, P.C.2
  • 41
    • 0037021802 scopus 로고    scopus 로고
    • Regulation of cell cycle and productivity in NS0 cells by the over-expression of p21
    • Watanabe S, Shuttleworth J, Al-Rubeai M (2002) Regulation of cell cycle and productivity in NS0 cells by the over-expression of p21. Biotechnol Bioeng 77:1-7
    • (2002) Biotechnol Bioeng , vol.77 , pp. 1-7
    • Watanabe, S.1    Shuttleworth, J.2    Al-Rubeai, M.3
  • 42
    • 0031974591 scopus 로고    scopus 로고
    • Ammonium ion transport by the Na+ K+ 2C1- co transporter induces apoptosis in hybridoma cells
    • Westlund A, Haggstrom L (1998) Ammonium ion transport by the Na+ K+ 2C1- co transporter induces apoptosis in hybridoma cells. Biotechnol Lett 20:87-90
    • (1998) Biotechnol Lett , vol.20 , pp. 87-90
    • Westlund, A.1    Haggstrom, L.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.