Development of stable cell lines for production or regulated expression using matrix attachment regions

Journal of Biotechnology

Volumn 87, Issue 1, 2001, Pages 29-42

  Zahn Zabal, Monique ^a   Kobr, Michel ^a   Girod, Pierre Alain ^a   Imhof, Markus ^a   Chatellard, Philippe ^a   De Jesus, Maria ^b   Wurm, Florian ^b   Mermod, Nicolas ^a  

^a UNIVERSITY OF LAUSANNE   (Switzerland)

^b EPFL   (Switzerland)

Author keywords

Gene expression; Matrix attachment region; Regulation; Transfection

Indexed keywords

BETA GALACTOSIDASE; LYSOZYME;

ANIMAL CELL; ARTICLE; CELL CLONE; CELL CLONING; CELL CULTURE; CHO CELL; CHROMATIN STRUCTURE; CONTROLLED STUDY; GENE CASSETTE; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE STRUCTURE; GENETIC SCREENING; GENETIC TRANSFECTION; MATRIX ATTACHMENT REGION; NONHUMAN; PLASMID; PRIORITY JOURNAL; REGULATOR GENE; REPORTER GENE; TRANSGENE;

ANIMALS; CELL LINE; CHICKENS; CHO CELLS; CHROMATIN; CRICETINAE; EXTRACELLULAR MATRIX; GENE EXPRESSION REGULATION; MURAMIDASE; PROTEIN ENGINEERING; TRANSFECTION; TRANSGENES;

CRICETINAE; CRICETULUS GRISEUS; GALLUS GALLUS;

EID: 0035957848     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(00)00423-5     Document Type: Article

References (37)

1. Scaffold attachment region-mediated enhancement of retroviral vector expression in primary T cells
2. High-level transgene expression in plant cells: Effects of a strong scaffold attachment region from tobacco
3. Stopped at the border: Boundaries and insulators
4. UASrpg can function as a heterochromatin boundary element in yeast
5. Scaffold/matrix-attached regions: Structural properties creating transcriptionally active loci
6. Transcriptional augmentation: Modulation of gene expression by scaffold/matrix-attached regions (S/MAR elements)
7. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: Polyethylenimine
8. Engineering passive immunity in transgenic mice secreting virus-neutralizing antibodies in milk
9. Identification of a class of chromatin boundary elements
10. Genetic optimization of recombinant glycoprotein production by mammalian cells
11. Activation by locus control regions
12. Facilitation of chromatin dynamics by SARs
13. A regulatory network for the efficient control of transgene expression
14. Extension of chromatin accessibility by nuclear matrix attachment regions
15. Transfecting mammalian cells: Optimization of critical parameters affecting calcium-phosphate precipitate formation
16. Position-independent transgene expression mediated by boundary elements from the apolipoprotein B chromatin domain
17. Amplification and expression of sequences cotransfected with a modular dihydrofolate reductase complementary DNA gene
18. Scaffold-attached regions from the human interferon β domain can be used to enhance the stable expression of genes under the control of various promoters
19. Construction of plasmids that express E. coli β-galactosidase in mammalian cells
20. Matrix-attachement regions can impart position-independent regulation of a tissue-specific gene in transgenic mice
21. CHO expression of a novel human recombinant IgG1 anti-Rh D antibody isolated by phage display
22. Stem cell gene therapy, position effects and chromatin insulators
23. Adjacent DNA elements dominantly restrict the ubiquitous activity of a novel chromatin-opening region to specific tissues
24. Retroviral vectors aimed at the gene therapy of human β-globin gene disorders
25. The matrix attachment regions of the chicken lysozyme gene co-map with the boundaries of the chromatin domain
26. The chicken lysozyme 5′ matrix attachment region increases transcription from a heterologous promoter in heterologous cells and dampens position effects on the expression of transfected genes
27. A vector based on the SV40 origin of replication and chromosomal S/MARs replicates episomally in CHO cells
28. SARs stimulate but do not confer position independent gene expression
29. Gene amplification and drug resistance in mammalian cells
30. A nuclear DNA attachment element mediates elevated and position-independent gene activity
31. The 5′ flanking region of the rat LAP (C/EPBβ) gene can direct high-level, position-independent, copy number-dependent expression in multiple tissues in transgenic mice
32. Dividing the empire: Boundary chromatin elements delimit the territory of enhancers
33. Deletion of the diploid dihydrofolate reductase locus from cultured mammalian cells
34. The chicken β-globin 5′HS4 boundary element blocks enhancer-mediated suppression of silencing
35. Ligand-inducible and liver-specific target gene expression in transgenic mice
36. Codon optimization, genetic insulation, and an rtTA reporter improve performance of the tetracycline switch
37. Integration and stability of CHO amplicons containing plasmid sequences