IRES-mediated Tricistronic vectors for enhancing generation of high monoclonal antibody expressing CHO cell lines

Journal of Biotechnology

Volumn 157, Issue 1, 2012, Pages 130-139

  Ho, Steven C L ^a   Bardor, Muriel ^a   Feng, Huatao ^a   Mariati, ^a   Tong, Yen Wah ^b   Song, Zhiwei ^a   Yap, Miranda G S ^a,b   Yang, Yuansheng ^a  

^a AGENCY FOR SCIENCE   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Aggregation; CHO; Glycosylation; IRES; Monoclonal antibody; Tricistronic

Indexed keywords

BATCH CULTURE; CHO; CHO CELL; GENE AMPLIFICATION; HEAVY CHAIN; HIGH PRODUCTIVITY; INTERNAL RIBOSOME ENTRY SITE; IRES; LIGHT CHAIN; NEOMYCIN-PHOSPHOTRANSFERASE; PRODUCT QUALITY; PROTEIN-FREE; SELECTION MARKER; SHAKE FLASKS; SPECIFIC PRODUCTIVITY; TRICISTRONIC;

AGGLOMERATION; BATCH CELL CULTURE; CELLS; CLONING; ESTERIFICATION; GENE EXPRESSION; GLYCOSYLATION; LAKES; MONOCLONAL ANTIBODIES; OPTIMIZATION; PRODUCTIVITY; SITE SELECTION;

VECTORS;

KANAMYCIN KINASE; MONOCLONAL ANTIBODY;

ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; CELL DENSITY; CELL VIABILITY; CHO CELL; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPRESSION VECTOR; GEL PERMEATION CHROMATOGRAPHY; GENE AMPLIFICATION; GENE EXPRESSION; GENETIC TRANSFECTION; HEAVY CHAIN; INTERNAL RIBOSOME ENTRY SITE; LIGHT CHAIN; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; PROTEIN PURIFICATION; TRICISTRONIC VECTOR; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, MONOCLONAL; CELL SURVIVAL; CHO CELLS; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; CRICETINAE; CRICETULUS; GENETIC VECTORS; GLYCOSYLATION; HUMANS; PARTICLE SIZE; PEPTIDE CHAIN INITIATION, TRANSLATIONAL; PLASMIDS; POLYSACCHARIDES; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; REGULATORY ELEMENTS, TRANSCRIPTIONAL; STAPHYLOCOCCAL PROTEIN A; TRANSFECTION;

EID: 84855237715     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.09.023     Document Type: Article

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