Meganuclease-driven targeted integration in CHO-K1 cells for the fast generation of HTS-compatible cell-based assays

Journal of Biomolecular Screening

Volumn 15, Issue 8, 2010, Pages 956-967

  Cabaniols, Jean Pierre ^a   Ouvry, Christine ^b   Lamamy, Véronique ^b   Fery, Isabelle ^b   Craplet, Marie Laure ^c   Moulharat, Natacha ^b   Guenin, Sophie Pénélope ^b   Bedut, Stéphane ^c   Nosjean, Olivier ^b   Ferry, Gilles ^b   Devavry, Séverine ^b   Jacqmarcq, Cécile ^a   Lebuhotel, Céline ^a   Mathis, Luc ^a   Delenda, Christophe ^a   Boutin, Jean A ^b   Duchâteau, Philippe ^a   Cogé, Francis ^b   Pâques, Frédéric ^a  

^a Arvid Wallgrens Backe 20   (France)

^b INSTITUT DE RECHERCHES SERVIER   (France)

^c INSTITUT DE RECHERCHES SERVIER   (France)

Author keywords

cell based assay; high throughput screening; homologous gene targeting; meganuclease; protein expression

Indexed keywords

AUTOTAXIN; BETA GALACTOSIDASE; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; CELL ASSAY; CELLULAR GENOME POSITIONING SYSTEM; CHO CELL; CONTROLLED STUDY; GENE SILENCING; GENE TARGETING; HIGH THROUGHPUT SCREENING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANIMALS; CELL LINE; CELLS; CHO CELLS; CHROMOSOME MAPPING; CRICETINAE; CRICETULUS; DEOXYRIBONUCLEASES; GENE TARGETING; HIGH-THROUGHPUT SCREENING ASSAYS; MODELS, BIOLOGICAL; MUTAGENESIS, SITE-DIRECTED; TIME FACTORS; TRANSFECTION;

CRICETULUS GRISEUS;

EID: 78449280396     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057110375115     Document Type: Article

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