Stepwise manipulation of DNA specificity in Flp recombinase: Progressively adapting Flp to individual and combinatorial mutations in its target site

Journal of Molecular Biology

Volumn 326, Issue 1, 2003, Pages 65-76

  Voziyanov, Yuri ^a   Konieczka, Jay H ^a   Stewart, A Francis ^b   Jayaram, Makkuni ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^b DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

Cre; Flp; Genomic engineering; Protein evolution; Site specific recombination

Indexed keywords

AMINO ACID; DNA; RECOMBINASE;

AMINO ACID ANALYSIS; ARTICLE; CONTROLLED STUDY; DNA DETERMINATION; ENZYME BINDING; GENE LIBRARY; GENE MUTATION; GENE TARGETING; GENETIC PARAMETERS; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0037423742     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-2836(02)01364-5     Document Type: Article

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