-
1
-
-
33746163862
-
Biopharmaceutical benchmarks 2006
-
Walsh G. Biopharmaceutical benchmarks 2006. Nat. Biotechnol. 24 (2006) 769-776
-
(2006)
Nat. Biotechnol.
, vol.24
, pp. 769-776
-
-
Walsh, G.1
-
2
-
-
8344271025
-
Advances in the production of human therapeutic proteins in yeasts and filamentous fungi
-
Gerngross T.U. Advances in the production of human therapeutic proteins in yeasts and filamentous fungi. Nat. Biotechnol. 22 (2004) 1409-1414
-
(2004)
Nat. Biotechnol.
, vol.22
, pp. 1409-1414
-
-
Gerngross, T.U.1
-
3
-
-
8344271026
-
Production of recombinant protein therapeutics in cultivated mammalian cells
-
Wurm F.M. Production of recombinant protein therapeutics in cultivated mammalian cells. Nat. Biotechnol. 22 (2004) 1393-1398
-
(2004)
Nat. Biotechnol.
, vol.22
, pp. 1393-1398
-
-
Wurm, F.M.1
-
4
-
-
23844433927
-
Animal cell cultures: recent achievements and perspectives in the production of biopharmaceuticals
-
Butler M. Animal cell cultures: recent achievements and perspectives in the production of biopharmaceuticals. Appl. Microbiol. Biotechnol. 68 (2005) 283-291
-
(2005)
Appl. Microbiol. Biotechnol.
, vol.68
, pp. 283-291
-
-
Butler, M.1
-
5
-
-
33745221421
-
Phenotypic variation during cloning procedures: analysis of the growth behavior of clonal cell lines
-
Barnes L.M., et al. Phenotypic variation during cloning procedures: analysis of the growth behavior of clonal cell lines. Biotechnol. Bioeng. 94 (2006) 530-537
-
(2006)
Biotechnol. Bioeng.
, vol.94
, pp. 530-537
-
-
Barnes, L.M.1
-
6
-
-
0035918616
-
Characterization of the stability of recombinant protein production in the GS-NS0 expression system
-
Barnes L.M., et al. Characterization of the stability of recombinant protein production in the GS-NS0 expression system. Biotechnol. Bioeng. 73 (2001) 261-270
-
(2001)
Biotechnol. Bioeng.
, vol.73
, pp. 261-270
-
-
Barnes, L.M.1
-
7
-
-
0035128469
-
Key determinants in the occurrence of clonal variation in humanized antibody expression of CHO cells during dihydrofolate reductase mediated gene amplification
-
Kim N.S., et al. Key determinants in the occurrence of clonal variation in humanized antibody expression of CHO cells during dihydrofolate reductase mediated gene amplification. Biotechnol. Prog. 17 (2001) 69-75
-
(2001)
Biotechnol. Prog.
, vol.17
, pp. 69-75
-
-
Kim, N.S.1
-
8
-
-
0025332641
-
Selective cloning of hybridoma cells for enhanced immunoglobulin production using flow cytometric cell sorting and automated laser nephelometry
-
Marder P., et al. Selective cloning of hybridoma cells for enhanced immunoglobulin production using flow cytometric cell sorting and automated laser nephelometry. Cytometry 11 (1990) 498-505
-
(1990)
Cytometry
, vol.11
, pp. 498-505
-
-
Marder, P.1
-
9
-
-
0008451224
-
Clonal variability within dihydrofolate reductase-mediated gene amplified Chinese hamster ovary cells: stability in the absence of selective pressure
-
Kim N.S., et al. Clonal variability within dihydrofolate reductase-mediated gene amplified Chinese hamster ovary cells: stability in the absence of selective pressure. Biotechnol. Bioeng. 60 (1998) 679-688
-
(1998)
Biotechnol. Bioeng.
, vol.60
, pp. 679-688
-
-
Kim, N.S.1
-
10
-
-
0025920324
-
Application of a population balance model to the loss of hybridoma antibody productivity
-
Lee G.M., et al. Application of a population balance model to the loss of hybridoma antibody productivity. Biotechnol. Prog. 7 (1991) 72-75
-
(1991)
Biotechnol. Prog.
, vol.7
, pp. 72-75
-
-
Lee, G.M.1
-
11
-
-
0028430190
-
Stability of producer hybridoma cell lines after cell sorting: a case study
-
Kromenaker S.J., and Srienc F. Stability of producer hybridoma cell lines after cell sorting: a case study. Biotechnol. Prog. 10 (1994) 299-307
-
(1994)
Biotechnol. Prog.
, vol.10
, pp. 299-307
-
-
Kromenaker, S.J.1
Srienc, F.2
-
13
-
-
0000291202
-
A rapid method for viable cell titration and clone production with HeLa cells in tissue culture: the use of X-irradiated cells to supply conditioning factors
-
Puck T.T., and Marcus P.I. A rapid method for viable cell titration and clone production with HeLa cells in tissue culture: the use of X-irradiated cells to supply conditioning factors. Proc. Natl. Acad. Sci. U. S. A. 41 (1955) 432-437
-
(1955)
Proc. Natl. Acad. Sci. U. S. A.
, vol.41
, pp. 432-437
-
-
Puck, T.T.1
Marcus, P.I.2
-
14
-
-
0023838813
-
Hazards of the limiting-dilution methods of cloning hybridomas
-
Underwood P.A., and Bean P.A. Hazards of the limiting-dilution methods of cloning hybridomas. J. Immunol. Methods 107 (1987) 119-128
-
(1987)
J. Immunol. Methods
, vol.107
, pp. 119-128
-
-
Underwood, P.A.1
Bean, P.A.2
-
15
-
-
0022556022
-
Poisson statistical analysis of repetitive sub-cloning by the limiting dilution technique as a way of assessing hybridoma monoclonality
-
Coller H.A., and Coller B.S. Poisson statistical analysis of repetitive sub-cloning by the limiting dilution technique as a way of assessing hybridoma monoclonality. Methods Enzymol. 121 (1986) 412-417
-
(1986)
Methods Enzymol.
, vol.121
, pp. 412-417
-
-
Coller, H.A.1
Coller, B.S.2
-
16
-
-
0025730563
-
Flow cytometric study of cultured mammalian cells
-
Al-Rubeai M., et al. Flow cytometric study of cultured mammalian cells. J. Biotechnol. 19 (1991) 67-81
-
(1991)
J. Biotechnol.
, vol.19
, pp. 67-81
-
-
Al-Rubeai, M.1
-
17
-
-
0013475072
-
Flow cytometry in animal culture
-
Al-Rubeai M., and Emery A.N. Flow cytometry in animal culture. Nat. Biotechnol. 11 (1993) 572-579
-
(1993)
Nat. Biotechnol.
, vol.11
, pp. 572-579
-
-
Al-Rubeai, M.1
Emery, A.N.2
-
18
-
-
27744540773
-
Dynamics of single cell property distributions in Chinese hamster ovary cell cultures monitored and controlled with automated flow cytometry
-
Kacmar J., and Srienc F. Dynamics of single cell property distributions in Chinese hamster ovary cell cultures monitored and controlled with automated flow cytometry. J. Biotechnol. 120 (2005) 410-420
-
(2005)
J. Biotechnol.
, vol.120
, pp. 410-420
-
-
Kacmar, J.1
Srienc, F.2
-
19
-
-
0033525428
-
A flow injection flow cytometry system for on-line monitoring of bioreactors
-
Zhao R., et al. A flow injection flow cytometry system for on-line monitoring of bioreactors. Biotechnol. Bioeng. 62 (1998) 609-617
-
(1998)
Biotechnol. Bioeng.
, vol.62
, pp. 609-617
-
-
Zhao, R.1
-
20
-
-
0025472073
-
Flow cytometric study of hybridoma cell culture: correlation between cell surface fluorescence and IgG production rate
-
Sen S., et al. Flow cytometric study of hybridoma cell culture: correlation between cell surface fluorescence and IgG production rate. Enzyme Microb. Technol. 12 (1990) 571-576
-
(1990)
Enzyme Microb. Technol.
, vol.12
, pp. 571-576
-
-
Sen, S.1
-
21
-
-
0038208103
-
A simple method for enriching populations of transfected CHO cells for cells of higher specific productivity
-
Brezinsky S.C.G., et al. A simple method for enriching populations of transfected CHO cells for cells of higher specific productivity. J. Immunol. Methods 277 (2003) 141-145
-
(2003)
J. Immunol. Methods
, vol.277
, pp. 141-145
-
-
Brezinsky, S.C.G.1
-
22
-
-
0024336762
-
Application of flow cytometric measurement of surface IgG in kinetic analysis of monoclonal antibody synthesis and secretion by murine hybridoma cells
-
Meilhoc E., et al. Application of flow cytometric measurement of surface IgG in kinetic analysis of monoclonal antibody synthesis and secretion by murine hybridoma cells. J. Immunol. Methods 121 (1989) 167-174
-
(1989)
J. Immunol. Methods
, vol.121
, pp. 167-174
-
-
Meilhoc, E.1
-
23
-
-
34247280567
-
Accelerated clone selection for recombinant CHO cells using a FACS-based high-throughput screen
-
DeMaria C.T., et al. Accelerated clone selection for recombinant CHO cells using a FACS-based high-throughput screen. Biotechnol. Prog. 23 (2007) 465-472
-
(2007)
Biotechnol. Prog.
, vol.23
, pp. 465-472
-
-
DeMaria, C.T.1
-
24
-
-
0031577267
-
Long-term, stable expression of green fluorescent protein in mammalian cells
-
Gubin A.N., et al. Long-term, stable expression of green fluorescent protein in mammalian cells. Biochem. Biophys. Res. Commun. 236 (1997) 347-350
-
(1997)
Biochem. Biophys. Res. Commun.
, vol.236
, pp. 347-350
-
-
Gubin, A.N.1
-
25
-
-
0033969002
-
Green fluorescent protein (GFP) as a second selectable marker for selection of high producing clones from transfected CHO cells
-
Meng Y.G., et al. Green fluorescent protein (GFP) as a second selectable marker for selection of high producing clones from transfected CHO cells. Gene 242 (2000) 201-207
-
(2000)
Gene
, vol.242
, pp. 201-207
-
-
Meng, Y.G.1
-
26
-
-
4143053500
-
Optimization of protein production in mammalian cells with a coexpressed fluorescent marker
-
Mancia F., et al. Optimization of protein production in mammalian cells with a coexpressed fluorescent marker. Structure 12 (2004) 1355-1360
-
(2004)
Structure
, vol.12
, pp. 1355-1360
-
-
Mancia, F.1
-
27
-
-
0035812441
-
Flow cytometry: an improved method for the selection of highly productive gene-amplified CHO cells using flow cytometry
-
Yoshikawa T., et al. Flow cytometry: an improved method for the selection of highly productive gene-amplified CHO cells using flow cytometry. Biotechnol. Bioeng. 74 (2001) 435-442
-
(2001)
Biotechnol. Bioeng.
, vol.74
, pp. 435-442
-
-
Yoshikawa, T.1
-
28
-
-
0022938612
-
Gel microdroplets for microbial measurement and screening: basic principles
-
Weaver J.C. Gel microdroplets for microbial measurement and screening: basic principles. Biotechnol. Bioeng. Symp. 17 (1986) 185-195
-
(1986)
Biotechnol. Bioeng. Symp.
, vol.17
, pp. 185-195
-
-
Weaver, J.C.1
-
29
-
-
0025267202
-
Rapid microbial detection and enumeration using gel microdroplets and colorimetric or fluorescence indicator systems
-
Williams G.B., et al. Rapid microbial detection and enumeration using gel microdroplets and colorimetric or fluorescence indicator systems. J. Clin. Microbiol. 28 (1990) 1002-1008
-
(1990)
J. Clin. Microbiol.
, vol.28
, pp. 1002-1008
-
-
Williams, G.B.1
-
30
-
-
2542466949
-
Isolation of rare isotype switch variants in hybridoma cell lines using an agarose gel microdrop-based protein secretion assay
-
Akselband Y., et al. Isolation of rare isotype switch variants in hybridoma cell lines using an agarose gel microdrop-based protein secretion assay. Assay Drug Dev. Technol. 1 (2003) 619-626
-
(2003)
Assay Drug Dev. Technol.
, vol.1
, pp. 619-626
-
-
Akselband, Y.1
-
31
-
-
0029029084
-
Secretion capture and report web: use of affinity derivatized agarose microdroplets for the selection of hybridoma cells
-
Gray F., et al. Secretion capture and report web: use of affinity derivatized agarose microdroplets for the selection of hybridoma cells. J. Immunol. Methods 182 (1994) 155-163
-
(1994)
J. Immunol. Methods
, vol.182
, pp. 155-163
-
-
Gray, F.1
-
32
-
-
0033735527
-
The gel microdrop secretion assay: identification of a low productivity subpopulation arising during the production of human antibody in CHO cells
-
Hammill L., et al. The gel microdrop secretion assay: identification of a low productivity subpopulation arising during the production of human antibody in CHO cells. Cytotechnology 34 (2000) 27-37
-
(2000)
Cytotechnology
, vol.34
, pp. 27-37
-
-
Hammill, L.1
-
33
-
-
0030904484
-
Gel microdrop technology for rapid isolation of rare and high producer cells
-
Weaver J.C., et al. Gel microdrop technology for rapid isolation of rare and high producer cells. Nat. Med. 3 (1997) 583-585
-
(1997)
Nat. Med.
, vol.3
, pp. 583-585
-
-
Weaver, J.C.1
-
34
-
-
11944254485
-
Gel microdroplets and flow cytometry: rapid determination of antibody secretion by individual cells within a cell population
-
Powell K.T., and Weaver J.C. Gel microdroplets and flow cytometry: rapid determination of antibody secretion by individual cells within a cell population. Biotechnology (N. Y.) 8 (1990) 333-337
-
(1990)
Biotechnology (N. Y.)
, vol.8
, pp. 333-337
-
-
Powell, K.T.1
Weaver, J.C.2
-
35
-
-
0032551664
-
Quantitating secretion rates of individual cells: design of secretion assays
-
Frykman S., and Srienc F. Quantitating secretion rates of individual cells: design of secretion assays. Biotechnol. Bioeng. 59 (1998) 214-226
-
(1998)
Biotechnol. Bioeng.
, vol.59
, pp. 214-226
-
-
Frykman, S.1
Srienc, F.2
-
36
-
-
0028960201
-
Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix
-
Manz R., et al. Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix. Proc. Natl. Acad. Sci. U. S. A. 92 (1995) 1921-1925
-
(1995)
Proc. Natl. Acad. Sci. U. S. A.
, vol.92
, pp. 1921-1925
-
-
Manz, R.1
-
37
-
-
0032758728
-
Improved cell line development by a high throughput affinity capture surface display technique to select for high secretors
-
Holmes P., and Al-Rubeai M. Improved cell line development by a high throughput affinity capture surface display technique to select for high secretors. J. Immunol. Methods 230 (1999) 141-147
-
(1999)
J. Immunol. Methods
, vol.230
, pp. 141-147
-
-
Holmes, P.1
Al-Rubeai, M.2
-
38
-
-
0034446598
-
Efficient selection of high-producing sub-clones during gene amplification of recombinant Chinese hamster ovary cells by flow cytometry and cell sorting
-
Borth N., et al. Efficient selection of high-producing sub-clones during gene amplification of recombinant Chinese hamster ovary cells by flow cytometry and cell sorting. Biotechnol. Bioeng. 71 (2000) 266-273
-
(2000)
Biotechnol. Bioeng.
, vol.71
, pp. 266-273
-
-
Borth, N.1
-
39
-
-
5544322216
-
High-throughput laser-mediated in situ cell purification with high purity and yield
-
Koller M.R., et al. High-throughput laser-mediated in situ cell purification with high purity and yield. Cytometry A 61A (2004) 153-161
-
(2004)
Cytometry A
, vol.61 A
, pp. 153-161
-
-
Koller, M.R.1
-
40
-
-
25144433398
-
Automated in situ measurement of cell-specific antibody secretion and laser-mediated purification for rapid cloning of highly-secreting producers
-
Hanania E.G., et al. Automated in situ measurement of cell-specific antibody secretion and laser-mediated purification for rapid cloning of highly-secreting producers. Biotechnol. Bioeng. 91 (2005) 872-876
-
(2005)
Biotechnol. Bioeng.
, vol.91
, pp. 872-876
-
-
Hanania, E.G.1
-
41
-
-
36348949333
-
High-throughput screening of cell lines expressing monoclonal antibodies
-
Lee C., et al. High-throughput screening of cell lines expressing monoclonal antibodies. Bioproc. Int. 4 Suppl. 3 (2006) 32-35
-
(2006)
Bioproc. Int.
, vol.4
, Issue.SUPPL. 3
, pp. 32-35
-
-
Lee, C.1
|