Selection of high-producing CHO cells using NPT selection marker with reduced enzyme activity

Biotechnology and Bioengineering

Volumn 89, Issue 5, 2005, Pages 530-538

  Sautter, Kerstin ^a,b   Enenkel, Barbara ^a  

^a B Ingelheim Pharma GmbH/Co KG   (Germany)

^b UNIVERSITY OF WÜRZBURG   (Germany)

Author keywords

Chinese hamster ovary (CHO); High expression system; MCP 1 (monocyte chemoattractant protein 1) production; Monoclonal antibody (mAb) production; Neomycin phosphotransferase (NPT)

Indexed keywords

AMINO ACIDS; BIOMARKERS; CHROMATES; ENZYME KINETICS; ENZYMES; GENES; RNA;

CLONE SCREENING; EXPRESSION SYSTEM; NEOMYCIN-PHOSPHOTRANSFERASE (NPT); TRANSCRIPTIONAL ACTIVITIES;

CELLS;

KANAMYCIN KINASE; RNA;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CELL COUNT; CELL POPULATION; CELL SURVIVAL; CHO CELL; CHROMATIN; CONTROLLED STUDY; ENZYME ACTIVITY; GENE EXPRESSION SYSTEM; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; MARKER GENE; MOLECULAR CLONING; MUTANT; NONHUMAN; POINT MUTATION; WILD TYPE;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; CELL SURVIVAL; CHEMOKINE CCL2; CHO CELLS; CLONE CELLS; CRICETINAE; CRICETULUS; GENE EXPRESSION; GENETIC MARKERS; GENTAMICINS; KANAMYCIN KINASE; MOLECULAR SEQUENCE DATA; POINT MUTATION; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SELECTION (GENETICS); TRANSFECTION;

CRICETINAE; CRICETULUS GRISEUS;

EID: 15944391987     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20374     Document Type: Article

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