Improving the efficiency of CHO cell line generation using glutamine synthetase gene knockout cells

Biotechnology and Bioengineering

Volumn 109, Issue 4, 2012, Pages 1007-1015

  Fan, Lianchun ^a   Kadura, Ibrahim ^a   Krebs, Lara E ^a   Hatfield, Christopher C ^a   Shaw, Margaret M ^a   Frye, Christopher C ^a  

^a ELI LILLY AND COMPANY   (United States)

Author keywords

Chinese hamster ovary cell; Glutamine synthetase; Knockout; Mammalian cell culture; Monoclonal antibody; Zinc finger nuclease

Indexed keywords

CHINESE HAMSTER OVARY CELLS; GLUTAMINE SYNTHETASE; KNOCKOUT; MAMMALIAN CELL CULTURE; ZINC FINGER NUCLEASE;

AMINO ACIDS; ANIMAL CELL CULTURE; CLONING; CYTOLOGY; EFFICIENCY; GENE EXPRESSION; INDUSTRIAL APPLICATIONS; MONOCLONAL ANTIBODIES; PRODUCTIVITY; RECOMBINANT PROTEINS; ZINC;

CLONE CELLS;

DIHYDROFOLATE REDUCTASE; GLUTAMATE AMMONIA LIGASE; METHIONINE SULFOXIMINE; ZINC FINGER PROTEIN;

ANIMAL CELL; ARTICLE; CELL CULTURE; CHO CELL; CHO CELL LINE GENERATION; CONTROLLED STUDY; NONHUMAN; PROTEIN EXPRESSION; PROTEIN FUNCTION;

ANIMALS; ANTIBODIES, MONOCLONAL; BATCH CELL CULTURE TECHNIQUES; CELL SEPARATION; CELL SURVIVAL; CHO CELLS; CLONE CELLS; CRICETINAE; CRICETULUS; DIPLOIDY; ENDODEOXYRIBONUCLEASES; EXONS; FLOW CYTOMETRY; GENE KNOCKOUT TECHNIQUES; GLUTAMATE-AMMONIA LIGASE; GLUTAMINE; METHIONINE SULFOXIMINE; POLYPLOIDY; RECOMBINANT FUSION PROTEINS; SELECTION, GENETIC; TRANSFECTION; ZINC FINGERS;

CRICETULUS GRISEUS; MAMMALIA;

EID: 84857444298     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24365     Document Type: Article

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