Improved recombinant protein yield using a codon deoptimized DHFR selectable marker in a CHEF1 expression plasmid

Biotechnology Progress

Volumn 26, Issue 6, 2010, Pages 1558-1566

  Westwood, Amy D ^a   Rowe, Daniel A ^a   Clarke, Howard R G ^a  

^a ELI LILLY AND COMPANY   (United States)

Author keywords

CHEF1; CHO; Codon deoptimization; DHFR; Recombinant protein expression

Indexed keywords

CHEF1; CHO; CODON DEOPTIMIZATION; DHFR; RECOMBINANT PROTEIN EXPRESSION;

CELL CULTURE; LAKES; PROTEINS; SITE SELECTION;

GENE EXPRESSION;

BIOLOGICAL MARKER; ELONGATION FACTOR 1; METHYLENETETRAHYDROFOLATE REDUCTASE (NADPH2); RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BIOSYNTHESIS; CELL CULTURE; CHEMISTRY; CHO CELL; CRICETULUS; GENETICS; HAMSTER; METABOLISM; MOLECULAR GENETICS; PLASMID; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

AMINO ACID SEQUENCE; ANIMALS; BIOLOGICAL MARKERS; CELLS, CULTURED; CHO CELLS; CRICETINAE; CRICETULUS; METHYLENETETRAHYDROFOLATE REDUCTASE (NADPH2); MOLECULAR SEQUENCE DATA; PEPTIDE ELONGATION FACTOR 1; PLASMIDS; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

CRICETINAE; CRICETULUS GRISEUS;

EID: 78650197691     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.491     Document Type: Article

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