Mammalian artificial chromosome pilot production facility: Large-scale isolation of functional satellite DNA-based artificial chromosomes

Cytometry

Volumn 35, Issue 2, 1999, Pages 129-133

  DeJong, Gary ^a   Telenius, Adèle H ^a   Telenius, Håkan ^a   Perez, Carl F ^a   Drayer, Jan I ^a   Hadlaczky, Gyula ^b  

^a Chromos Molecular Systems Inc   (Canada)

^b INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

Author keywords

Artificial chromosomes; Chromosome sorting; SATAC artificial chromosomes

Indexed keywords

ANIMAL CELL; ARTICLE; CELL CULTURE; CHROMOSOME CONDENSATION; CHROMOSOME PREPARATION; DNA DETERMINATION; FLOW CYTOMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEIN TARGETING;

ANIMALS; CELL LINE; CHO CELLS; CHROMOMYCINS; CHROMOSOMES; CRICETINAE; DNA, SATELLITE; FIBROBLASTS; FLOW CYTOMETRY; GENETIC TECHNIQUES; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; INFERTILITY; KARYOTYPING; MICE; PILOT PROJECTS;

ANIMALIA; MAMMALIA; RODENTIA;

EID: 0033081317     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19990201)35:2<129::AID-CYTO4>3.0.CO;2-A     Document Type: Article

References (12)

1. Harrington JJ, Bokkelen GV, Mays RW, Gustashaw K, Willard HF. Formation for de novo centromeres and construction of first-generation human artificial microchromosomes. Nat Genet 1997;15: 345-355.
2. Sun TQ, Fenstermacher D, Vos JMH. Human artificial episomal chromosomes for cloning large DNA in human cells. Nat Genet 1994;8:33-41.
3. Featherstone T, Huxley O. Extrachromosomal maintenance and amplification of yeast artificial chromosomes. Genomics 1993;17:267-278.
4. Holló G, Keresò J, Praznovszky T, Cserpán I, Fodor K, Katona R, Csonka E, Fátyol K, Szeles A, Szalay AA, Hadlaczky G. Evidence for a megareplicon covering megabases of centomeric chromosome segments. Chromosome Res 1996;4:240-247.
5. Keresö J, Praznovsky T, Cserpán I, Fodor K, Katona R, Csonka E, Fátyol K, Holló G, Szeles A, Ross AR, Sumner AT, Szalay AA, Hadlaczky G. De novo chromosome formation by large-scale amplification of the centromeric region of mouse chromosomes. Chromosome Res 1996; 4:226-239.
6. Gray JW, Carrano AV, Steinmetz LL, Van Dilla MA, Moore DH, Mayall BH, Mendelsohn ML. Chromosome measurement and sorting by flow systems. Proc Nat Acad Sci USA 1975;72:1231-1234.
7. Sillar R, Young BD. A new method for the preparation of metaphase chromosomes for flow analysis. J Histochem Cytochem 1981;29:74-79.
8. Telenius H, Szeles A, Keresö J, Csonka E, Praznovszky T, Imren S, Maxwell A, Perez CF, Drayer JI, Hadlaczky G. Stability of a functional murine satellite DNA-based artificial chromosome across mammalian species. Chromosome Res 1999: in press.
9. Cram LS, Campbell M, Fawcett JJ, Deaven LL. Polyamine buffer for bivariate human flow cytogenetic analysis and sorting. Methods Cell Biol 1990;33:369-376.
10. Van den Engh GJ, Trask BJ, Lansdorp P, Gray JW. Improved resolution of flow cytometric measurements of Hoechst- and chromomycin-A3-stained human chromosomes after addition of citrate and sulfite. Cytometry 1988;9:266-270.
11. Pinkel D, Straume T, Gray J. Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc Nat Acad Sci USA 1986;83:9138-9142.
12. Telenius H, de Vos D, Blennow E, Willat LR, Ponder BA, Carter NP. Chromatid contamination can impair the purity of flow-sorted metaphase chromosomes. Cytometry 1993;14:97-101.