Decreasing lactate level and increasing antibody production in Chinese Hamster Ovary cells (CHO) by reducing the expression of lactate dehydrogenase and pyruvate dehydrogenase kinases

Journal of Biotechnology

Volumn 153, Issue 1-2, 2011, Pages 27-34

  Zhou, Meixia ^a   Crawford, Yongping ^a   Ng, Domingos ^a   Tung, Jack ^a   Pynn, Abigail F J ^a   Meier, Angela ^a   Yuk, Inn H ^a   Vijayasankaran, Natarajan ^a   Leach, Kimberly ^a   Joly, John ^a   Snedecor, Bradley ^a   Shen, Amy ^a  

^a GENENTECH INC   (United States)

Author keywords

CHO; LDH and PDHK; Simultaneous knockdown; SiRNA

Indexed keywords

ACETYL-COA; ANTIBODY PRODUCTION; CELL CULTURE MEDIUMS; CHINESE HAMSTER OVARY CELLS; CHO; CHO CELL; COMMERCIAL PRODUCTIONS; FED BATCHES; FED-BATCH CULTURES; LACTATE DEHYDROGENASE; LDH AND PDHK; OSMOLALITY; PROTEIN PRODUCTION; PYRUVATE DEHYDROGENASE KINASE; PYRUVATES; SHAKE FLASKS; SIMULTANEOUS KNOCKDOWN; SIRNA; SPECIFIC PRODUCTIVITY; TCA CYCLE; THERAPEUTIC MONOCLONAL ANTIBODIES; THERAPEUTIC PROTEIN;

ALLERGIES; BIOREACTORS; CELL CULTURE; CELL GROWTH; CLONE CELLS; ENZYMES; GENE EXPRESSION; GROWTH KINETICS; MANUFACTURE; MONOCLONAL ANTIBODIES; PHOSPHORYLATION; PRODUCTIVITY; SURFACE PLASMON RESONANCE;

BATCH CELL CULTURE;

LACTATE DEHYDROGENASE; LACTIC ACID; MONOCLONAL ANTIBODY; PYRUVATE DEHYDROGENASE KINASE; SMALL INTERFERING RNA;

ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; BIOREACTOR; CELL GROWTH; CHO CELL; CLONE; CONTROLLED STUDY; DOWN REGULATION; FED BATCH CULTURE; FEMALE; GENE EXPRESSION; GENE SILENCING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS;

ADENOSINE TRIPHOSPHATE; ANIMALS; ANTIBODY FORMATION; BIOREACTORS; CELL PROLIFERATION; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENETIC VECTORS; GLUCOSE; HYDROGEN-ION CONCENTRATION; INTRACELLULAR SPACE; L-LACTATE DEHYDROGENASE; LACTIC ACID; PROTEIN-SERINE-THREONINE KINASES; RNA, MESSENGER; RNA, SMALL INTERFERING; TITRIMETRY;

CRICETULUS GRISEUS;

EID: 79955540196     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.03.003     Document Type: Article

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