Utilization of site-specific recombination for generating therapeutic protein producing cell lines

Molecular Biotechnology

Volumn 45, Issue 3, 2010, Pages 199-202

  Campbell, Margie ^a   Corisdeo, Susanne ^a   McGee, Clair ^a   Kraichely, Denny ^a  

^a CENTOCOR INC   (United States)

Author keywords

Chinese hamster ovary (CHO) stable cell lines; Mammalian cell line development; Serine recombinase; Site specific recombination; Targeted integration

Indexed keywords

CHINESE HAMSTER OVARY; MAMMALIAN CELL LINES; SERINE RECOMBINASE; SITE-SPECIFIC RECOMBINATION; STABLE CELL LINES; TARGETED INTEGRATION;

AMINO ACIDS; CELL CULTURE; DNA SEQUENCES; GENES; MAMMALS; PHOSPHATASES;

CELLS;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; GENE EXPRESSION; GENE LOCUS; GENETIC RECOMBINATION; GENETIC TRANSCRIPTION; NONHUMAN;

ANIMALS; ANTIBODIES, MONOCLONAL; ATTACHMENT SITES, MICROBIOLOGICAL; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; CRICETULUS; GENETIC VECTORS; PLASMIDS; RECOMBINANT PROTEINS; RECOMBINASES; RECOMBINATION, GENETIC;

CRICETULUS GRISEUS; MAMMALIA;

EID: 77954928400     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-010-9266-5     Document Type: Article

References (6)

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4. Smith, M. C., & Thorpe, H. M. (2002). Diversity in the serine recombinases. Molecular Microbiology, 44(2), 299-307.
5. Russell, J. P., Chang, D. W., Tretiakova, A., & Padidam, M. (2006). Phage Bxb1 integrase mediates highly efficient sitespecific recombination in mammalian cells. BioTechniques, 40, 460-464.
6. Patent: Site-specific serine recombinases and methods of their use. U.S. Provisional Patent Application 20080020465. Filed 08/20/ 2007. Inventors: Padidam, M.