Illuminating the life of GPCRs

Cell Communication and Signaling

Volumn 7, Issue , 2009, Pages

  Böhme, Ilka ^a   Beck Sickinger, Annette G ^a  

^a UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 78650172215     PISSN: None     EISSN: 1478811X     Source Type: Journal    
DOI: 10.1186/1478-811X-7-16     Document Type: Review

References (222)

1. G protein-coupled receptor interacting proteins: emerging roles in localization and signal transduction. AE Brady, LE Limbird, Cell Signal 2002 14 297 309 11858937
2. The role of beta-arrestins in the termination and transduction of G-protein-coupled receptor signals. LM Luttrell, RJ Lefkowitz, J Cell Sci 2002 115 455 465 11861753
3. Peptide receptors as molecular targets for cancer diagnosis and therapy. JC Reubi, Endocr Rev 2003 24 389 427 12920149
4. Creating new fluorescent probes for cell biology. J Zhang, RE Campbell, AY Ting, RY Tsien, Nat Rev Mol Cell Biol 2002 3 906 918 12461557
5. The fluorescent toolbox for assessing protein location and function. BN Giepmans, SR Adams, MH Ellisman, RY Tsien, Science 2006 312 217 224 16614209
6. Exploring membrane organization and dynamics by the wavelength-selective fluorescence approach. A Chattopadhyay, Chem Phys Lipids 2003 122 3 17 12598034
7. Membrane-dependent conformational changes initiate cholesterol-dependent cytolysin oligomerization and intersubunit beta-strand alignment. R Ramachandran, RK Tweten, AE Johnson, Nat Struct Mol Biol 2004 11 697 705 15235590
8. Nascent membrane and secretory proteins differ in FRET-detected folding far inside the ribosome and in their exposure to ribosomal proteins. CA Woolhead, PJ McCormick, AE Johnson, Cell 2004 116 725 736 15006354
9. Quantitative imaging in live human cells reveals intracellular alpha(1)-adrenoceptor ligand-binding sites. JF Mackenzie, CJ Daly, JD Pediani, JC McGrath, J Pharmacol Exp Ther 2000 294 434 443 10900216
10. Single-molecule spectroscopic methods. E Haustein, P Schwille, Curr Opin Struct Biol 2004 14 531 540 15465312
11. Imaging single events at the cell membrane. JK Jaiswal, SM Simon, Nat Chem Biol 2007 3 92 98 17235347
12. Total internal reflection fluorescence microscopy. D Axelrod, Methods Cell Biol 1989 30 245 270 2648112
13. Real-time measurement of exocytosis and endocytosis using interference of light. A Llobet, V Beaumont, L Lagnado, Neuron 2003 40 1075 1086 14687543
14. Lighting up the cell surface with evanescent wave microscopy. D Toomre, DJ Manstein, Trends Cell Biol 2001 11 298 303 11413041
15. RP Haugland, The Handbook-A Guide to Fluorescent Probes and Labeling Technologies Eugene: Molecular Probes 2005
16. Fluorescent labels for proteomics and genomics. A Waggoner, Curr Opin Chem Biol 2006 10 62 66 16418012
17. Intracellular trafficking pathways and drug delivery: fluorescence imaging of living and fixed cells. P Watson, AT Jones, DJ Stephens, Adv Drug Deliv Rev 2005 57 43 61 15518920
18. Molecular determinants underlying the formation of stable intracellular G protein-coupled receptor-beta-arrestin complexes after receptor endocytosis*. RH Oakley, SA Laporte, JA Holt, LS Barak, MG Caron, J Biol Chem 2001 276 19452 19460 11279203
19. Mutation of tyrosine in the conserved NPXXY sequence leads to constitutive phosphorylation and internalization, but not signaling, of the human B2 bradykinin receptor. I Kalatskaya, S Schussler, A Blaukat, W Muller-Esterl, M Jochum, D Proud, A Faussner, J Biol Chem 2004 279 31268 31276 15161928
20. Characterisation of G-protein-coupled receptors by antibodies. CP Eckard, AG Beck-Sickinger, Curr Med Chem 2000 7 897 910 10911022
21. The use of phage display peptide libraries for basic and translational research. R Brissette, NI Goldstein, Methods Mol Biol 2007 383 203 213 18217687
22. A beta-arrestin binding determinant common to the second intracellular loops of rhodopsin family G protein-coupled receptors. S Marion, RH Oakley, KM Kim, MG Caron, LS Barak, J Biol Chem 2006 281 2932 2938 16319069
23. Homologous desensitization of signalling by the beta (beta) isoform of the human thromboxane A2 receptor. LP Kelley-Hickie, BT Kinsella, Biochim Biophys Acta 2006 1761 1114 1131 16956790
24. Diverse functional motifs within the three intracellular loops of the CGRP1 receptor. AC Conner, J Simms, MT Conner, DL Wootten, M Wheatley, DR Poyner, Biochemistry 2006 45 12976 12985 17059214
25. Role of the C terminus in neuropeptide Y Y1 receptor desensitization and internalization. ND Holliday, CW Lam, IR Tough, HM Cox, Mol Pharmacol 2005 67 655 664 15576634
26. Rapid agonist-induced desensitization and internalization of the A(2B) adenosine receptor is mediated by a serine residue close to the COOH terminus. AL Matharu, SJ Mundell, JL Benovic, E Kelly, J Biol Chem 2001 276 30199 30207 11333255
27. Importance of constitutive activity and arrestin-independent mechanisms for intracellular trafficking of the ghrelin receptor. ND Holliday, B Holst, EA Rodionova, TW Schwartz, HM Cox, Mol Endocrinol 2007 21 3100 3112 17717076
28. Lipid raft-dependent glucagon-like peptide-2 receptor trafficking occurs independently of agonist-induced desensitization. JL Estall, B Yusta, DJ Drucker, Mol Biol Cell 2004 15 3673 3687 15169869
29. Complementary roles of the DRY motif and C-terminus tail of GPCRS for G protein coupling and beta-arrestin interaction. KM Kim, MG Caron, Biochem Biophys Res Commun 2008 366 42 47 18036556
30. Internalization of sst2, sst3, and sst5 receptors: effects of somatostatin agonists and antagonists. R Cescato, S Schulz, B Waser, V Eltschinger, JE Rivier, HJ Wester, M Culler, M Ginj, Q Liu, A Schonbrunn, JC Reubi, J Nucl Med 2006 47 502 511 16513620
31. Agonist-selective mechanisms of mu-opioid receptor desensitization in human embryonic kidney 293 cells. EA Johnson, S Oldfield, E Braksator, A Gonzalez-Cuello, D Couch, KJ Hall, SJ Mundell, CP Bailey, E Kelly, G Henderson, Mol Pharmacol 2006 70 676 685 16682505
32. Unraveling G protein-coupled receptor endocytosis pathways using real-time monitoring of agonist-promoted interaction between beta-arrestins and AP-2. FF Hamdan, MD Rochdi, B Breton, D Fessart, DE Michaud, PG Charest, SA Laporte, M Bouvier, J Biol Chem 2007 282 29089 29100 17675294
33. Solubilization and display of G protein-coupled receptors on beads for real-time fluorescence and flow cytometric analysis. LA Sklar, J Vilven, E Lynam, D Neldon, TA Bennett, E Prossnitz, BioTechniques 2000 28 976 980 10818705
34. CypHer 5: a generic approach for measuring the activation and trafficking of G protein-coupled receptors in live cells. EJ Adie, MJ Francis, J Davies, L Smith, A Marenghi, C Hather, K Hadingham, NP Michael, G Milligan, S Game, Assay Drug Dev Technol 2003 1 251 259 15090190
35. Distinct phosphorylation sites in the SST2A somatostatin receptor control internalization, desensitization, and arrestin binding. Q Liu, DA Dewi, W Liu, MS Bee, A Schonbrunn, Mol Pharmacol 2008 73 292 304 17981995
36. Intracellular cross-talk between the GPCR CXCR1 and CXCR2: role of carboxyl terminus phosphorylation sites. H Attal, E Cohen-Hillel, T Meshel, JM Wang, W Gong, A Ben-Baruch, Exp Cell Res 2008 314 352 365 17996233
37. CXCR1 and CXCR2 activation and regulation. Role of aspartate 199 of the second extracellular loop of CXCR2 in CXCL8-mediated rapid receptor internalization. MW Nasser, SK Raghuwanshi, KM Malloy, P Gangavarapu, JY Shim, K Rajarathnam, RM Richardson, J Biol Chem 2007 282 6906 6915 17204468
38. Beta-arrestin binding to CC chemokine receptor 5 requires multiple C-terminal receptor phosphorylation sites and involves a conserved Asp-Arg-Tyr sequence motif. F Huttenrauch, A Nitzki, FT Lin, S Honing, M Oppermann, J Biol Chem 2002 277 30769 30777 12065593
39. Effect of inactivating mutations on phosphorylation and internalization of the human VPAC2 receptor. I Langer, C Langlet, P Robberecht, J Mol Endocrinol 2005 34 405 414 15821106
40. Contribution of the carboxyl terminus of the VPAC1 receptor to agonist-induced receptor phosphorylation, internalization, and recycling. C Langlet, I Langer, P Vertongen, N Gaspard, JM Vanderwinden, P Robberecht, J Biol Chem 2005 280 28034 28043 15932876
41. Agonist- and antagonist-induced sequestration/internalization of neuropeptide Y Y1 receptors in HEK293 cells. LH Pheng, Y Dumont, A Fournier, JG Chabot, A Beaudet, R Quirion, Br J Pharmacol 2003 139 695 704 12812992
42. Functional and molecular properties of the human recombinant Y4 receptor: resistance to agonist-promoted desensitization. T Voisin, M Goumain, AM Lorinet, JJ Maoret, M Laburthe, J Pharmacol Exp Ther 2000 292 638 646 10640301
43. Intracellular trafficking of angiotensin II and its AT1 and AT2 receptors: evidence for selective sorting of receptor and ligand. L Hein, L Meinel, RE Pratt, VJ Dzau, BK Kobilka, Mol Endocrinol 1997 11 1266 1277 9259318
44. Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. O Shimomura, FH Johnson, Y Saiga, J Cell Comp Physiol 1962 59 223 239 13911999
45. Primary structure of the Aequorea victoria green-fluorescent protein. DC Prasher, VK Eckenrode, WW Ward, FG Prendergast, MJ Cormier, Gene 1992 111 229 233 1347277
46. Green fluorescent protein as a marker for gene expression. M Chalfie, Y Tu, G Euskirchen, WW Ward, DC Prasher, Science 1994 263 802 805 8303295
47. The discovery of aequorin and green fluorescent protein. O Shimomura, J Microsc 2005 217 1 15 15655058
48. The green fluorescent protein. RY Tsien, Annu Rev Biochem 1998 67 509 544 9759496
49. Go with the glow: fluorescent proteins to light transgenic organisms. CN Stewart Jr, Trends Biotechnol 2006 24 155 162 16488034
50. Ca2+ activation of hSlo K+ channel is suppressed by N-terminal GFP tag. E Meyer, P Fromherz, Eur J Neurosci 1999 11 1105 1108 10103104
51. Fusion to GFP blocks intercellular trafficking of the sucrose transporter SUT1 leading to accumulation in companion cells. S Lalonde, A Weise, RP Walsh, JM Ward, WB Frommer, BMC Plant Biol 2003 3 8 14667250
52. Green fluorescent protein (GFP): applications, structure, and related photophysical behavior. M Zimmer, Chem Rev 2002 102 759 781 11890756
53. Efficiently folding and circularly permuted variants of the Sapphire mutant of GFP. O Zapata-Hommer, O Griesbeck, BMC Biotechnol 2003 3 5 12769828
54. Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. DA Zacharias, JD Violin, AC Newton, RY Tsien, Science 2002 296 913 916 11988576
55. An improved cyan fluorescent protein variant useful for FRET. MA Rizzo, GH Springer, B Granada, DW Piston, Nat Biotechnol 2004 22 445 449 14990965
56. Evolutionary optimization of fluorescent proteins for intracellular FRET. AW Nguyen, PS Daugherty, Nat Biotechnol 2005 23 355 360 15696158
57. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. T Nagai, K Ibata, ES Park, M Kubota, K Mikoshiba, A Miyawaki, Nat Biotechnol 2002 20 87 90 11753368
58. Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications. O Griesbeck, GS Baird, RE Campbell, DA Zacharias, RY Tsien, J Biol Chem 2001 276 29188 29194 11387331
59. Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. S Karasawa, T Araki, T Nagai, H Mizuno, A Miyawaki, Biochem J 2004 381 307 312 15065984
60. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. NC Shaner, RE Campbell, PA Steinbach, BN Giepmans, AE Palmer, RY Tsien, Nat Biotechnol 2004 22 1567 1572 15558047
61. Evolution of new nonantibody proteins via iterative somatic hypermutation. L Wang, WC Jackson, PA Steinbach, RY Tsien, Proc Natl Acad Sci USA 2004 101 16745 16749 15556995
62. Green fluorescent proteins in receptor research: an emerging tool for drug discovery. KH Arun, CL Kaul, P Ramarao, J Pharmacol Toxicol Methods 2005 51 1 23 15596111
63. Fluorescent proteins as a toolkit for in vivo imaging. DM Chudakov, S Lukyanov, KA Lukyanov, Trends Biotechnol 2005 23 605 613 16269193
64. Evolution of function and color in GFP-like proteins. MV Matz, YA Labas, J Ugalde, Methods Biochem Anal 2006 47 139 161 16342417
65. Engineering and characterization of a superfolder green fluorescent protein. JD Pedelacq, S Cabantous, T Tran, TC Terwilliger, GS Waldo, Nat Biotechnol 2006 24 79 88 16369541
66. A guide to choosing fluorescent proteins. NC Shaner, PA Steinbach, RY Tsien, Nat Methods 2005 2 905 909 16299475
67. Five-colour in vivo imaging of neurons in Caenorhabditis elegans. H Hutter, J Microsc 2004 215 213 218 15315508
68. A new configuration of the Zeiss LSM 510 for simultaneous optical separation of green and red fluorescent protein pairs. KI Anderson, J Sanderson, S Gerwig, J Peychl, Cytometry A 2006 69 920 929 16969813
69. Recent advances using green and red fluorescent protein variants. A Muller-Taubenberger, KI Anderson, Appl Microbiol Biotechnol 2007 77 1 12 17704916
70. Fluorescent proteins as sensors for cellular functions. O Griesbeck, Curr Opin Neurobiol 2004 14 636 641 15464898
71. "Fluorescent timer": protein that changes color with time. A Terskikh, A Fradkov, G Ermakova, A Zaraisky, P Tan, AV Kajava, X Zhao, S Lukyanov, M Matz, S Kim, I Weissman, P Siebert, Science 2000 290 1585 1588 11090358
72. Red, yellow, green go!-A novel tool for microscopic segregation of secretory vesicle pools according to their age. UK Wiegand, RR Duncan, J Greaves, RH Chow, MJ Shipston, DK Apps, Biochem Soc Trans 2003 31 851 856 12887320
73. Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis. CD Hu, TK Kerppola, Nat Biotechnol 2003 21 539 545 12692560
74. Nanoscale resolution in GFP-based microscopy. KI Willig, RR Kellner, R Medda, B Hein, S Jakobs, SW Hell, Nat Methods 2006 3 721 723 16896340
75. FRET imaging. EA Jares-Erijman, TM Jovin, Nat Biotechnol 2003 21 1387 1395 14595367
76. Imaging protein molecules using FRET and FLIM microscopy. H Wallrabe, A Periasamy, Curr Opin Biotechnol 2005 16 19 27 15722011
77. The use of resonance energy transfer in high-throughput screening: BRET versus FRET. N Boute, R Jockers, T Issad, Trends Pharmacol Sci 2002 23 351 354 12377570
78. Bimolecular fluorescence complementation (BiFC) analysis as a probe of protein interactions in living cells. TK Kerppola, Annu Rev Biophys 2008 37 465 487 18573091
79. Visualization of molecular interactions by fluorescence complementation. TK Kerppola, Nat Rev Mol Cell Biol 2006 7 449 456 16625152
80. Tracking of human Y receptors in living cells-a fluorescence approach. I Bohme, K Morl, D Bamming, C Meyer, AG Beck-Sickinger, Peptides 2007 28 226 234 17207557
81. Specific covalent labeling of recombinant protein molecules inside live cells. BA Griffin, SR Adams, RY Tsien, Science 1998 281 269 272 9657724
82. Site-specific incorporation of fluorescent probes into protein: hexahistidine-tag-mediated fluorescent labeling with (Ni(2+):nitrilotriacetic Acid (n)-fluorochrome conjugates. AN Kapanidis, YW Ebright, RH Ebright, J Am Chem Soc 2001 123 12123 12125 11724636
83. Reversible site-selective labeling of membrane proteins in live cells. EG Guignet, R Hovius, H Vogel, Nat Biotechnol 2004 22 440 444 15034592
84. A general method for the covalent labeling of fusion proteins with small molecules in vivo. A Keppler, S Gendreizig, T Gronemeyer, H Pick, H Vogel, K Johnsson, Nat Biotechnol 2003 21 86 89 12469133
85. Labeling of fusion proteins with synthetic fluorophores in live cells. A Keppler, H Pick, C Arrivoli, H Vogel, K Johnsson, Proc Natl Acad Sci USA 2004 101 9955 9959 15226507
86. Specific labeling of cell surface proteins with chemically diverse compounds. N George, H Pick, H Vogel, N Johnsson, K Johnsson, J Am Chem Soc 2004 126 8896 8897 15264811
87. Visualizing odorant receptor trafficking in living cells down to the single-molecule level. V Jacquier, M Prummer, JM Segura, H Pick, H Vogel, Proc Natl Acad Sci USA 2006 103 14325 14330 16980412
88. The HaloTag: a novel technology for cell imaging and protein analysis. GV Los, K Wood, Methods Mol Biol 2007 356 195 208 16988404
89. Spatial separation and bidirectional trafficking of proteins using a multi-functional reporter. S Svendsen, C Zimprich, MG McDougall, DH Klaubert, GV Los, BMC Cell Biol 2008 9 17 18384686
90. Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase. I Chen, M Howarth, W Lin, AY Ting, Nat Methods 2005 2 99 104 15782206
91. Site-specific labeling of proteins with small molecules in live cells. I Chen, AY Ting, Curr Opin Biotechnol 2005 16 35 40 15722013
92. Fluorophores for live cell imaging of AGT fusion proteins across the visible spectrum. A Keppler, C Arrivoli, L Sironi, J Ellenberg, BioTechniques 2006 41 167 170 16925018
93. A FlAsH-based FRET approach to determine G protein-coupled receptor activation in living cells. C Hoffmann, G Gaietta, M Bunemann, SR Adams, S Oberdorff-Maass, B Behr, JP Vilardaga, RY Tsien, MH Ellisman, MJ Lohse, Nat Methods 2005 2 171 176 15782185
94. SNAP-tag mediated live cell labeling as an alternative to GFP in anaerobic organisms. A Regoes, AB Hehl, BioTechniques 2005 39 809 810 16382896
95. Fluorescein-labeled naloxone binding to mu opioid receptors on live Chinese hamster ovary cells using confocal fluorescent microscopy. BW Madsen, CL Beglan, CE Spivak, J Neurosci Methods 2000 97 123 131 10788666
96. Differently labeled peptide ligands for rapid investigation of receptor expression on a new human glioblastoma cell line. M Fabry, C Cabrele, H Hocker, AG Beck-Sickinger, Peptides 2000 21 1885 1893 11150651
97. Differential PI 3-kinase dependence of early and late phases of recycling of the internalized AT1 angiotensin receptor. L Hunyady, AJ Baukal, Z Gaborik, JA Olivares-Reyes, M Bor, M Szaszak, R Lodge, KJ Catt, T Balla, J Cell Biol 2002 157 1211 1222 12070129
98. MUSK, a new target for mutations causing congenital myasthenic syndrome. F Chevessier, B Faraut, A Ravel-Chapuis, P Richard, K Gaudon, S Bauche, C Prioleau, R Herbst, E Goillot, C Ioos, JP Azulay, S Attarian, JP Leroy, E Fournier, C Legay, L Schaeffer, J Koenig, M Fardeau, B Eymard, J Pouget, D Hantai, Hum Mol Genet 2004 13 3229 3240 15496425
99. Single-molecule imaging of EGFR signalling on the surface of living cells. Y Sako, S Minoghchi, T Yanagida, Nat Cell Biol 2000 2 168 172 10707088
100. Monitoring expression and clustering of the ionotropic 5HT3 receptor in plasma membranes of live biological cells. H Pick, AK Preuss, M Mayer, T Wohland, R Hovius, H Vogel, Biochemistry 2003 42 877 884 12549905
101. BODIPY-conjugated neuropeptide Y ligands: new fluorescent tools to tag Y1, Y2, Y4 and Y5 receptor subtypes. Y Dumont, P Gaudreau, M Mazzuferi, D Langlois, JG Chabot, A Fournier, M Simonato, R Quirion, Br J Pharmacol 2005 146 1069 1081 16231000
102. Fluorescent pirenzepine derivatives as potential bitopic ligands of the human M1 muscarinic receptor. C Tahtaoui, I Parrot, P Klotz, F Guillier, JL Galzi, M Hibert, B Ilien, J Med Chem 2004 47 4300 4315 15294002
103. Dynamics of beta2-adrenergic receptor-ligand complexes on living cells. O Hegener, L Prenner, F Runkel, SL Baader, J Kappler, H Haberlein, Biochemistry 2004 43 6190 6199 15147203
104. Synthetic chemokines directly labeled with a fluorescent dye as tools for studying chemokine and chemokine receptor interactions. AE Strong, AC Thierry, P Cousin, C Moulon, S Demotz, Eur Cytokine Netw 2006 17 49 59 16613763
105. Fluorescence imaging in vivo: recent advances. J Rao, A Dragulescu-Andrasi, H Yao, Curr Opin Biotechnol 2007 18 17 25 17234399
106. Quantum dots and peptides: a bright future together. M Zhou, I Ghosh, Biopolymers 2007 88 325 339 17167795
107. Peptide-labeled quantum dots for imaging GPCRs in whole cells and as single molecules. M Zhou, E Nakatani, LS Gronenberg, T Tokimoto, MJ Wirth, VJ Hruby, A Roberts, RM Lynch, I Ghosh, Bioconjug Chem 2007 18 323 332 17373766
108. Photophysics of dopamine-modified quantum dots and effects on biological systems. SJ Clarke, CA Hollmann, Z Zhang, D Suffern, SE Bradforth, NM Dimitrijevic, WG Minarik, JL Nadeau, Nat Mater 2006 5 409 417 16617348
109. Differences in the subcellular localization of alpha1-adrenoceptor subtypes can affect the subtype selectivity of drugs in a study with the fluorescent ligand BODIPY FL-prazosin. T Sugawara, A Hirasawa, K Hashimoto, G Tsujimoto, Life Sci 2002 70 2113 2124 12002804
110. De novo identification of highly active fluorescent kappa opioid ligands from a rhodamine labeled tetrapeptide positional scanning library. RA Houghten, CT Dooley, JR Appel, Bioorg Med Chem Lett 2004 14 1947 1951 15050634
111. Hessian-free low-mode conformational search for large-scale protein loop optimization: application to c-jun N-terminal kinase JNK3. I Kolossváry, GM Keserü J Comput Chem 2001 22 21 30
112. Synthesis and characterization of the first fluorescent nonpeptide NPY Y1 receptor antagonist. E Schneider, M Keller, A Brennauer, BK Hoefelschweiger, D Gross, OS Wolfbeis, G Bernhardt, A Buschauer, Chembiochem 2007 8 1981 1988 17876753
113. Cluster formation of opiate (enkephalin) receptors in neuroblastoma cells: differences between agonists and antagonists and possible relationships to biological functions. E Hazum, KJ Chang, P Cuatrecasas, Proc Natl Acad Sci USA 1980 77 3038 3041 6248885
114. Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. MG Gustafsson, J Microsc 2000 198 82 87 10810003
115. I5M: 3D widefield light microscopy with better than 100 nm axial resolution. MG Gustafsson, DA Agard, JW Sedat, J Microsc 1999 195 10 16 10444297
116. Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. MN Bobrow, TD Harris, KJ Shaughnessy, GJ Litt, J Immunol Methods 1989 125 279 285 2558138
117. Tyramide signal amplification method in multiple-label immunofluorescence confocal microscopy. G Wang, CL Achim, RL Hamilton, CA Wiley, V Soontornniyomkij, Methods 1999 18 459 464 10491275
118. The antigen-antibody reaction in immunohistochemistry. C Montero, J Histochem Cytochem 2003 51 1 4 12502748
119. Plasma membrane localization and functional rescue of truncated forms of a G protein-coupled receptor. T Schoneberg, J Liu, J Wess, J Biol Chem 1995 270 18000 18006 7629108
120. Receptor subtype-specific docking of Asp6.59 with C-terminal arginine residues in Y receptor ligands. N Merten, D Lindner, N Rabe, H Rompler, K Morl, T Schoneberg, AG Beck-Sickinger, J Biol Chem 2007 282 7543 7551 17204471
121. Differential agonist-mediated internalization of the human 5-hydroxytryptamine 7 receptor isoforms. CR Guthrie, AT Murray, AA Franklin, MW Hamblin, J Pharmacol Exp Ther 2005 313 1003 1010 15716386
122. Binding and internalization of fluorescent opioid peptide conjugates in living cells. S Arttamangkul, V Alvarez-Maubecin, G Thomas, JT Williams, DK Grandy, Mol Pharmacol 2000 58 1570 1580 11093798
123. Cellular localization and pharmacological characterization of functioning alpha-1 adrenoceptors by fluorescent ligand binding and image analysis reveals identical binding properties of clustered and diffuse populations of receptors. CJ Daly, CM Milligan, G Milligan, JF Mackenzie, JC McGrath, J Pharmacol Exp Ther 1998 286 984 990 9694959
124. Importance of agonists in alpha-adrenoceptor classification and localisation of alpha1-adrenoceptors in human prostate. JC McGrath, MA Naghadeh, JD Pediani, JF Mackenzie, CJ Daly, Eur Urol 1999 36 Suppl 1 80 88 10393479
125. Measurement of cellular and tissue distribution of beta-adrenoceptors in rat thoracic aorta using BODIPY-CGP 12177. N Brahmadevara, A MacDonald, JC McGrath, CJ Daly, Br J Pharmacol 2002 137 93P
126. Visualising agonist and antagonist interactions; The value of fluorescent ligands. CJ Daly, JD Pediani, JF Mackenzie, JC McGrath, The Pharmacologist 2002 44
127. Implications for bcd mRNA localization from spatial distribution of exu protein in Drosophila oogenesis. S Wang, T Hazelrigg, Nature 1994 369 400 403 7910952
128. Quantitative in vivo microscopy: the return from the 'omics'. R Fernandez-Gonzalez, A Munoz-Barrutia, MH Barcellos-Hoff, C Ortiz-de-Solorzano, Curr Opin Biotechnol 2006 17 501 510 16899361
129. Visualization of G protein-coupled receptor trafficking with the aid of the green fluorescent protein. Endocytosis and recycling of cholecystokinin receptor type A. NI Tarasova, RH Stauber, JK Choi, EA Hudson, G Czerwinski, JL Miller, GN Pavlakis, CJ Michejda, SA Wank, J Biol Chem 1997 272 14817 14824 9169450
130. Polarized cell surface expression of the green fluorescent protein-tagged vasopressin V2 receptor in Madin Darby canine kidney cells. R Schulein, D Lorenz, A Oksche, B Wiesner, R Hermosilla, J Ebert, W Rosenthal, FEBS Lett 1998 441 170 176 9883878
131. Lipid rafts: heterogeneity on the high seas. LJ Pike, Biochem J 2004 378 281 292 14662007
132. Shrinking patches and slippery rafts: scales of domains in the plasma membrane. M Edidin, Trends Cell Biol 2001 11 492 496 11719055
133. Membrane domains. S Mukherjee, FR Maxfield, Annu Rev Cell Dev Biol 2004 20 839 866 15473862
134. Detergents as tools for the purification and classification of lipid rafts. LH Chamberlain, FEBS Lett 2004 559 1 5 14986659
135. A GFP fluorescence-based approach to determine detergent insolubility of the human serotonin1A receptor. S Kalipatnapu, A Chattopadhyay, FEBS Lett 2004 576 455 460 15498580
136. The evolving role of lipid rafts and caveolae in G protein-coupled receptor signaling: implications for molecular pharmacology. RS Ostrom, PA Insel, Br J Pharmacol 2004 143 235 245 15289291
137. G protein-coupled receptor signalling and cross-talk: achieving rapidity and specificity. EM Hur, KT Kim, Cell Signal 2002 14 397 405 11882384
138. Fluorescence photobleaching analysis for the study of cellular dynamics. N Klonis, M Rug, I Harper, M Wickham, A Cowman, L Tilley, Eur Biophys J 2002 31 36 51 12046896
139. Internal trafficking and surface mobility of a functionally intact beta2-adrenergic receptor-green fluorescent protein conjugate. LS Barak, SS Ferguson, J Zhang, C Martenson, T Meyer, MG Caron, Mol Pharmacol 1997 51 177 184 9203621
140. Characterization of an intrinsically fluorescent gonadotropin-releasing hormone receptor and effects of ligand binding on receptor lateral diffusion. S Nelson, RD Horvat, J Malvey, DA Roess, BG Barisas, CM Clay, Endocrinology 1999 140 950 957 9927328
141. Structural and functional aspects of G protein-coupled receptor oligomerization. TE Hebert, M Bouvier, Biochem Cell Biol 1998 76 1 11 9666301
142. Neurotransmitter receptor dynamics studied in vivo by reversible photo-unbinding of fluorescent ligands. M Akaaboune, RM Grady, S Turney, JR Sanes, JW Lichtman, Neuron 2002 34 865 876 12086635
143. Regulation of CB1 cannabinoid receptor internalization by a promiscuous phosphorylation-dependent mechanism. TL Daigle, ML Kwok, K Mackie, J Neurochem 2008 106 70 82 18331587
144. Receptor/beta-arrestin complex formation and the differential trafficking and resensitization of beta2-adrenergic and angiotensin II type 1A receptors. PH Anborgh, JL Seachrist, LB Dale, SS Ferguson, Mol Endocrinol 2000 14 2040 2053 11117533
145. The glucagon-like peptide-2 receptor C terminus modulates beta-arrestin-2 association but is dispensable for ligand-induced desensitization, endocytosis, and G-protein-dependent effector activation. JL Estall, JA Koehler, B Yusta, DJ Drucker, J Biol Chem 2005 280 22124 22134 15817468
146. Association of beta-arrestin with G protein-coupled receptors during clathrin-mediated endocytosis dictates the profile of receptor resensitization. RH Oakley, SA Laporte, JA Holt, LS Barak, MG Caron, J Biol Chem 1999 274 32248 32257 10542263
147. Exploring the dynamics of regulation of G protein-coupled receptors using green fluorescent protein. G Milligan, Br J Pharmacol 1999 128 501 510 10516625
148. Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins. J Llopis, JM McCaffery, A Miyawaki, MG Farquhar, RY Tsien, Proc Natl Acad Sci USA 1998 95 6803 6808 9618493
149. Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins. G Miesenbock, DA De Angelis, JE Rothman, Nature 1998 394 192 195 9671304
150. Fluorescent ligands for studying neuropeptide receptors by confocal microscopy. A Beaudet, D Nouel, T Stroh, F Vandenbulcke, C Dal-Farra, JP Vincent, Braz J Med Biol Res 1998 31 1479 1489 9921286
151. Pharmacology and functional properties of NTS2 neurotensin receptors in cerebellar granule cells. P Sarret, L Gendron, P Kilian, HM Nguyen, N Gallo-Payet, MD Payet, A Beaudet, J Biol Chem 2002 277 36233 36243 12084713
152. The regulatory mechanisms of export trafficking of G protein-coupled receptors. MT Duvernay, CM Filipeanu, G Wu, Cell Signal 2005 17 1457 1465 16014327
153. Subtype-specific intracellular trafficking of alpha2-adrenergic receptors. DA Daunt, C Hurt, L Hein, J Kallio, F Feng, BK Kobilka, Mol Pharmacol 1997 51 711 720 9145909
154. A photoactivatable GFP for selective photolabeling of proteins and cells. GH Patterson, J Lippincott-Schwartz, Science 2002 297 1873 1877 12228718
155. Photoswitchable cyan fluorescent protein for protein tracking. DM Chudakov, VV Verkhusha, DB Staroverov, EA Souslova, S Lukyanov, KA Lukyanov, Nat Biotechnol 2004 22 1435 1439 15502815
156. EosFP, a fluorescent marker protein with UV-inducible green-to-red fluorescence conversion. J Wiedenmann, S Ivanchenko, F Oswald, F Schmitt, C Rocker, A Salih, KD Spindler, GU Nienhaus, Proc Natl Acad Sci USA 2004 101 15905 15910 15505211
157. Photoconversion of YFP into a CFP-like species during acceptor photobleaching FRET experiments. G Valentin, C Verheggen, T Piolot, H Neel, M Coppey-Moisan, E Bertrand, Nat Methods 2005 2 801 16278647
158. Spinning disk confocal microscope system for rapid high-resolution, multimode, fluorescence speckle microscopy and green fluorescent protein imaging in living cells. PS Maddox, B Moree, JC Canman, ED Salmon, Meth Enzymol 2003 360 597 617 12622170
159. Multi-photon microscopy: seeing more by imaging less. N White, R Errington, BioTechniques 2002 33 298 300 302, 304-295 12188180
160. Distribution plasticity of the human estrogen receptor alpha in live cells: distinct imaging of consecutively expressed receptors. H Pick, H Jankevics, H Vogel, J Mol Biol 2007 374 1213 1223 17991486
161. Fluorescent ligands for the study of receptors. JC McGrath, S Arribas, CJ Daly, Trends Pharmacol Sci 1996 17 393 399 8990953
162. Fluorescent ligands, antibodies, and proteins for the study of receptors. CJ Daly, JC McGrath, Pharmacol Ther 2003 100 101 118 14609715
163. Flow cytometry: a novel approach for the quantitative analysis of receptor-ligand interactions on surfaces of living cells. B Bohn, Mol Cell Endocrinol 1980 20 1 15 6254819
164. A simple and powerful flow cytometric method for the simultaneous determination of multiple parameters at G protein-coupled receptor subtypes. E Schneider, M Mayer, R Ziemek, L Li, C Hutzler, G Bernhardt, A Buschauer, Chembiochem 2006 7 1400 1409 16888730
165. Fluorescence correlation spectroscopy for the detection and study of single molecules in biology. MA Medina, P Schwille, Bioessays 2002 24 758 764 12210537
166. Pharmacology under the microscope: the use of fluorescence correlation spectroscopy to determine the properties of ligand-receptor complexes. SJ Briddon, SJ Hill, Trends Pharmacol Sci 2007 28 637 645 18001848
167. Fluorescence polarization and anisotropy in high throughput screening: perspectives and primer. JC Owicki, J Biomol Screen 2000 5 297 306 11080688
168. The neuropeptide Y monomer in solution is not folded in the pancreatic-polypeptide fold. A Bettio, MC Dinger, AG Beck-Sickinger, Protein Sci 2002 11 1834 1844 12070335
169. Conformation state-sensitive antibodies to G-protein-coupled receptors. A Gupta, FM Decaillot, I Gomes, O Tkalych, AS Heimann, ES Ferro, LA Devi, J Biol Chem 2007 282 5116 5124 17148456
170. Optical techniques to analyze real-time activation and signaling of G-protein-coupled receptors. MJ Lohse, VO Nikolaev, P Hein, C Hoffmann, JP Vilardaga, M Bunemann, Trends Pharmacol Sci 2008 29 159 165 18262662
171. The neurokinin A receptor activates calcium and cAMP responses through distinct conformational states. T Palanche, B Ilien, S Zoffmann, MP Reck, B Bucher, SJ Edelstein, JL Galzi, J Biol Chem 2001 276 34853 34861 11459843
172. Turn-on switch in parathyroid hormone receptor by a two-step parathyroid hormone binding mechanism. M Castro, VO Nikolaev, D Palm, MJ Lohse, JP Vilardaga, Proc Natl Acad Sci USA 2005 102 16084 16089 16236727
173. Measurement of the millisecond activation switch of G protein-coupled receptors in living cells. JP Vilardaga, M Bunemann, C Krasel, M Castro, MJ Lohse, Nat Biotechnol 2003 21 807 812 12808462
174. Real-time optical recording of beta1-adrenergic receptor activation reveals supersensitivity of the Arg389 variant to carvedilol. F Rochais, JP Vilardaga, VO Nikolaev, M Bunemann, MJ Lohse, S Engelhardt, J Clin Invest 2007 117 229 235 17200720
175. Gs activation is time-limiting in initiating receptor-mediated signaling. P Hein, F Rochais, C Hoffmann, S Dorsch, VO Nikolaev, S Engelhardt, CH Berlot, MJ Lohse, M Bunemann, J Biol Chem 2006 281 33345 33351 16963443
176. Probing the activation-promoted structural rearrangements in preassembled receptor-G protein complexes. C Gales, JJ Van Durm, S Schaak, S Pontier, Y Percherancier, M Audet, H Paris, M Bouvier, Nat Struct Mol Biol 2006 13 778 786 16906158
177. Receptor-mediated activation of heterotrimeric G-proteins in living cells. C Janetopoulos, T Jin, P Devreotes, Science 2001 291 2408 2411 11264536
178. Gi protein activation in intact cells involves subunit rearrangement rather than dissociation. M Bunemann, M Frank, MJ Lohse, Proc Natl Acad Sci USA 2003 100 16077 16082 14673086
179. Fluorescent biosensor for quantitative real-time measurements of inositol 1,4,5-trisphosphate in single living cells. A Tanimura, A Nezu, T Morita, RJ Turner, Y Tojyo, J Biol Chem 2004 279 38095 38098 15272011
180. A genetically encoded, fluorescent indicator for cyclic AMP in living cells. M Zaccolo, F De Giorgi, CY Cho, L Feng, T Knapp, PA Negulescu, SS Taylor, RY Tsien, T Pozzan, Nat Cell Biol 2000 2 25 29 10620803
181. Detecting cAMP-induced Epac activation by fluorescence resonance energy transfer: Epac as a novel cAMP indicator. B Ponsioen, J Zhao, J Riedl, F Zwartkruis, G van der Krogt, M Zaccolo, WH Moolenaar, JL Bos, K Jalink, EMBO Rep 2004 5 1176 1180 15550931
182. Spatiotemporal dynamics of guanosine 3',5'-cyclic monophosphate revealed by a genetically encoded, fluorescent indicator. A Honda, SR Adams, CL Sawyer, V Lev-Ram, RY Tsien, WR Dostmann, Proc Natl Acad Sci USA 2001 98 2437 2442 11226257
183. Fluorescent sensors for rapid monitoring of intracellular cGMP. VO Nikolaev, S Gambaryan, MJ Lohse, Nat Methods 2006 3 23 25 16369548
184. Single fluorescent protein-based Ca2+ sensors with increased dynamic range. EA Souslova, VV Belousov, JG Lock, S Stromblad, S Kasparov, AP Bolshakov, VG Pinelis, YA Labas, S Lukyanov, LM Mayr, DM Chudakov, BMC Biotechnol 2007 7 37 17603870
185. New methodologies for measuring protein interactions in vivo and in vitro. J Piehler, Curr Opin Struct Biol 2005 15 4 14 15718127
186. Fluorescence resonance energy transfer analysis of cell surface receptor interactions and signaling using spectral variants of the green fluorescent protein. FK Chan, RM Siegel, D Zacharias, R Swofford, KL Holmes, RY Tsien, MJ Lenardo, Cytometry 2001 44 361 368 11500853
187. Plug flow cytometry extends analytical capabilities in cell adhesion and receptor pharmacology. BS Edwards, FW Kuckuck, ER Prossnitz, A Okun, JT Ransom, LA Sklar, Cytometry 2001 43 211 216 11170109
188. Imaging protein-protein interactions using fluorescence resonance energy transfer microscopy. AK Kenworthy, Methods 2001 24 289 296 11403577
189. New technologies: bioluminescence resonance energy transfer (BRET) for the detection of real time interactions involving G-protein coupled receptors. KD Pfleger, KA Eidne, Pituitary 2003 6 141 151 14974443
190. Monitoring of ligand-independent dimerization and ligand-induced conformational changes of melatonin receptors in living cells by bioluminescence resonance energy transfer. MA Ayoub, C Couturier, E Lucas-Meunier, S Angers, P Fossier, M Bouvier, R Jockers, J Biol Chem 2002 277 21522 21528 11940583
191. Molecular imaging of drug-modulated protein-protein interactions in living subjects. R Paulmurugan, TF Massoud, J Huang, SS Gambhir, Cancer Res 2004 64 2113 2119 15026351
192. Optical imaging of Renilla luciferase reporter gene expression in living mice. S Bhaumik, SS Gambhir, Proc Natl Acad Sci USA 2002 99 377 382 11752410
193. G-protein-coupled receptor oligomerization and its potential for drug discovery. SR George, BF O'Dowd, SP Lee, Nat Rev Drug Discov 2002 1 808 820 12360258
194. Functional consequences of 7TM receptor dimerization. JL Hansen, SP Sheikh, Eur J Pharm Sci 2004 23 301 317 15567283
195. Roles of G-protein-coupled receptor dimerization. S Terrillon, M Bouvier, EMBO Rep 2004 5 30 34 14710183
196. Detecting and imaging protein-protein interactions during G protein-mediated signal transduction in vivo and in situ by using fluorescence-based techniques. TE Hebert, C Gales, RV Rebois, Cell Biochem Biophys 2006 45 85 109 16679566
197. G-protein-coupled receptor dimerization: modulation of receptor function. CD Rios, BA Jordan, I Gomes, LA Devi, Pharmacol Ther 2001 92 71 87 11916530
198. Cell-surface protein-protein interaction analysis with time-resolved FRET and snap-tag technologies: application to GPCR oligomerization. D Maurel, L Comps-Agrar, C Brock, ML Rives, E Bourrier, MA Ayoub, H Bazin, N Tinel, T Durroux, L Prezeau, E Trinquet, JP Pin, Nat Methods 2008 5 561 567 18488035
199. FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells. BH Meyer, JM Segura, KL Martinez, R Hovius, N George, K Johnsson, H Vogel, Proc Natl Acad Sci USA 2006 103 2138 2143 16461466
200. Photobleaching fluorescence resonance energy transfer reveals ligand-induced oligomer formation of human somatostatin receptor subtypes. RC Patel, DC Lange, YC Patel, Methods 2002 27 340 348 12217650
201. Ligand binding to somatostatin receptors induces receptor-specific oligomer formation in live cells. RC Patel, U Kumar, DC Lamb, JS Eid, M Rocheville, M Grant, A Rani, T Hazlett, SC Patel, E Gratton, YC Patel, Proc Natl Acad Sci USA 2002 99 3294 3299 11880655
202. Real-time monitoring of receptor and G-protein interactions in living cells. C Gales, RV Rebois, M Hogue, P Trieu, A Breit, TE Hebert, M Bouvier, Nat Methods 2005 2 177 184 15782186
203. Subunit interactions of GTP-binding proteins. H Heithier, M Frohlich, C Dees, M Baumann, M Haring, P Gierschik, E Schiltz, WL Vaz, M Hekman, EJ Helmreich, Eur J Biochem 1992 204 1169 1181 1312936
204. Visualization of a functional Galpha q-green fluorescent protein fusion in living cells. Association with the plasma membrane is disrupted by mutational activation and by elimination of palmitoylation sites, but not be activation mediated by receptors or AlF4. TE Hughes, H Zhang, DE Logothetis, CH Berlot, J Biol Chem 2001 276 4227 4235 11076942
205. Live cell imaging of Gs and the beta2-adrenergic receptor demonstrates that both alphas and beta1gamma7 internalize upon stimulation and exhibit similar trafficking patterns that differ from that of the beta2-adrenergic receptor. TR Hynes, SM Mervine, EA Yost, JL Sabo, CH Berlot, J Biol Chem 2004 279 44101 44112 15297467
206. Intracellular trafficking of hormone receptors. Z Gaborik, L Hunyady, Trends Endocrinol Metab 2004 15 286 293 15358282
207. Detection of beta 2-adrenergic receptor dimerization in living cells using bioluminescence resonance energy transfer (BRET). S Angers, A Salahpour, E Joly, S Hilairet, D Chelsky, M Dennis, M Bouvier, Proc Natl Acad Sci USA 2000 97 3684 3689 10725388
208. The use of bioluminescence resonance energy transfer 2 to study neuropeptide Y receptor agonist-induced beta-arrestin 2 interaction. MM Berglund, DA Schober, MA Statnick, PH McDonald, DR Gehlert, J Pharmacol Exp Ther 2003 306 147 156 12665544
209. Oxytocin and vasopressin V1a and V2 receptors form constitutive homo- and heterodimers during biosynthesis. S Terrillon, T Durroux, B Mouillac, A Breit, MA Ayoub, M Taulan, R Jockers, C Barberis, M Bouvier, Mol Endocrinol 2003 17 677 691 12554793
210. Characterization of sequence determinants within the carboxyl-terminal domain of chemokine receptor CCR5 that regulate signaling and receptor internalization. K Kraft, H Olbrich, I Majoul, M Mack, A Proudfoot, M Oppermann, J Biol Chem 2001 276 34408 34418 11448957
211. Real-time detection of interactions between the human oxytocin receptor and G protein-coupled receptor kinase-2. A Hasbi, D Devost, SA Laporte, HH Zingg, Mol Endocrinol 2004 18 1277 1286 14976224
212. Agonist induced receptor internalization of neuropeptide Y receptor subtypes depends on third intracellular loop and C-terminus. I Bohme, J Stichel, C Walther, K Morl, AG Beck-Sickinger, Cell Signal 2008 20 1740 1749 18598760
213. Site-specific incorporation of keto amino acids into functional G protein-coupled receptors using unnatural amino acid mutagenesis. S Ye, C Kohrer, T Huber, M Kazmi, P Sachdev, EC Yan, A Bhagat, UL RajBhandary, TP Sakmar, J Biol Chem 2008 283 1525 1533 17993461
214. Monovalent, reduced-size quantum dots for imaging receptors on living cells. M Howarth, W Liu, S Puthenveetil, Y Zheng, LF Marshall, MM Schmidt, KD Wittrup, MG Bawendi, AY Ting, Nat Methods 2008 5 397 399 18425138
215. Binding proteins selected from combinatorial libraries of an alpha-helical bacterial receptor domain. K Nord, E Gunneriusson, J Ringdahl, S Stahl, M Uhlen, PA Nygren, Nat Biotechnol 1997 15 772 777 9255793
216. Cellular studies of binding, internalization and retention of a radiolabeled EGFR-binding affibody molecule. E Nordberg, M Friedman, L Gostring, GP Adams, H Brismar, FY Nilsson, S Stahl, B Glimelius, J Carlsson, Nucl Med Biol 2007 34 609 618 17707800
217. Development of a baculovirus-based fluorescence resonance energy transfer assay for measuring protein-protein interaction. TC Cheung, JP Hearn, Eur J Biochem 2003 270 4973 4981 14653823
218. G-protein coupled receptor oligomerization in neuroendocrine pathways. KM Kroeger, KD Pfleger, KA Eidne, Front Neuroendocrinol 2003 24 254 278 14726257
219. A syntaxin homolog encoded by VAM3 mediates down-regulation of a yeast G protein-coupled receptor. CJ Stefan, KJ Blumer, J Biol Chem 1999 274 1835 1841 9880567
220. odr-10 encodes a seven transmembrane domain olfactory receptor required for responses to the odorant diacetyl. P Sengupta, JH Chou, CI Bargmann, Cell 1996 84 899 909 8601313
221. Whole-cell recordings from preoptic/hypothalamic slices reveal burst firing in gonadotropin-releasing hormone neurons identified with green fluorescent protein in transgenic mice. KJ Suter, JP Wuarin, BN Smith, FE Dudek, SM Moenter, Endocrinology 2000 141 3731 3736 11014229
222. The color of mice: in the light of GFP-variant reporters. AK Hadjantonakis, A Nagy, Histochem Cell Biol 2001 115 49 58 11219608