I5M: 3D widefield light microscopy with better than 100 nm axial resolution

Journal of Microscopy

Volumn 195, Issue 1, 1999, Pages 10-16

  Gustafsson, M G L ^a   Agard, D A ^a   Sedat, J W ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Cytoskeleton; Deconvolution; I(n)M; I2M; I5M; Interference; Microtubule; Optical microscopy; OTF; Resolution; Superresolution; Widefield microscopy

Indexed keywords

FLUORESCENCE MICROSCOPY; OPTICAL MICROSCOPY;

CYTOSKELETONS; DECONVOLUTIONS; I(N)M; I2M; I5M; INTERFERENCE; MICROTUBULES; OTF; RESOLUTION; SUPERRESOLUTION; WIDE-FIELD MICROSCOPIES;

INTERFEROMETRY;

ARTICLE; CELL ULTRASTRUCTURE; CYTOSKELETON; IMAGE ENHANCEMENT; MICROSCOPY; MICROTUBULE; PRIORITY JOURNAL; ULTRASTRUCTURE;

CYTOSKELETON; MICROSCOPY; MICROTUBULES;

EID: 0032864460     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.1999.00576.x     Document Type: Article

References (12)

1. Abbe, E. (1873) Beiträge zür Theorie des Mikroskops und der mikroskopischen Wahrnehmung. Arkiv Mikroskopische Anal. 9, 413-468.
2. Agard, D.A., Hiraoka, Y., Shaw, P. & Sedat, J.W. (1989) Fluorescence microscopy in three dimensions. Methods Cell Biol. 30, 353-377.
3. Bailey, B., Farkas, D.L., Taylor, D.L. & Lanni, F. (1993) Enhancement of axial resolution in fluorescence microscopy by standing-wave excitation. Nature, 366, 44-48.
4. Gustafsson, M.G.L., Agard, D.A. & Sedat, J.W. (1995) Sevenfold improvement of axial resolution in 3D widefield microscopy using two objective lenses, Proc. SPIE, 2412, 147-156.
5. Gustafsson, M.G.L., Acard, D.A. & Sedat, J.W. (1996) 3D widefield microscopy with two objective lenses: experimental verification of improved axial resolution. Proc. SPIE, 2655, 62-66.
6. Hell, S.W., Schrader, M. & van der Voort, H.T.M. (1997) Far-field fluorescence microscopy with three-dimensional resolution in the 100-nm range, J. Microsc. 187, 1-7.
7. Hell, S. & Stelzer, E.H.K, (1992) Properties of a 4Pi confocal fluorescence microscope. J. Opt. Soc. Am. A, 9, 2159-2166.
8. Lanni, F., Bailey, B., Farkas, D.L. & Taylor, D.L. (1993) Excitation field synthesis as a means for obtaining enhanced axial resolution in fluorescence microscopes. Bioimaging, 1, 187-196.
9. Pawley, J.B., ed. (1990) Handbook of Biological Confocal Microscopy. Plenum Press, New York.
10. Reasenberg, R.D., ed. (1998) Astronomical interferometry. Proc. SPIE, 3350, entire volume.
11. Schrader, M., Hell, S.W. & van der Voort, H.T.M. (1998) Three-dimensional super-resolution with a 4Pi-confocal microscope using image restoration. J. Appl. Phys. 84, 4033-4042.
12. Streibl, N. (1985) Three-dimensional imaging by a microscope. J. Opt. Soc. Am. A, 2, 121-127.