Quantitative imaging in live human cells reveals intracellular α1- adrenoceptor ligand-binding sites

Journal of Pharmacology and Experimental Therapeutics

Volumn 294, Issue 2, 2000, Pages 434-443

  Mackenzie, Janet F ^a   Daly, Craig J ^a   Pediani, John D ^a   McGrath, John C ^a  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

2 [[2 (2,6 DIMETHOXYPHENOXY)ETHYL]AMINOMETHYL] 1,4 BENZODIOXAN; 5 METHYL 3 [3 [4 [2 (2,2,2 TRIFLUOROETHOXY)PHENYL] 1 PIPERAZINYL]PROPYL] 1H PYRIMIDINE 2,4 DIONE; 5 METHYLURAPIDIL; 8 [2 [4 (2 METHOXYPHENYL) 1 PIPERAZINYL]ETHYL] 8 AZASPIRO[4.5]DECANE 7,9 DIONE; ALPHA 1 ADRENERGIC RECEPTOR; DOXAZOSIN; LIGAND; N [5 (4,5 DIHYDRO 1H IMIDAZOL 2 YL) 5,6,7,8 TETRAHYDRO 2 HYDROXY 1 NAPHTHYL]METHANESULFONAMIDE; PRAZOSIN; RECEPTOR SUBTYPE; TAMSULOSIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; BINDING COMPETITION; CELLULAR DISTRIBUTION; FLUORESCENCE; HUMAN; HUMAN CELL; IMAGING; LASER MICROSCOPY; LIGAND BINDING; MEMBRANE BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; QUANTITATIVE ASSAY; SMOOTH MUSCLE FIBER;

ADRENERGIC ALPHA-ANTAGONISTS; ANIMALS; BINDING SITES; BINDING, COMPETITIVE; BORON COMPOUNDS; CELL MEMBRANE; FLUORESCENT DYES; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; KINETICS; LIGANDS; MALE; MICROSCOPY, CONFOCAL; MUSCLE, SMOOTH; PRAZOSIN; PROSTATE; RADIOLIGAND ASSAY; RECEPTORS, ADRENERGIC, ALPHA-1; SUBCELLULAR FRACTIONS; TRITIUM;

EID: 0033913768     PISSN: 00223565     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Al-Damluji S, Porter D, Krsmanovic LZ, Knutson JR and Kopin IJ (1997) Visual detection of transport-P in peptidergic neurones. Br J Pharmacol 120:876-882.
2. Avakian OV and Gillespie JS (1968) Uptake of noradrenaline by adrenergic nerve, smooth muscle and connective tissue in isolated perfused arteries and its correlation with the vasoconstrictor response. Br J Pharmacol Chemother 32:168-184.
3. 50) of an enzymatic reaction. Biochem Pharmacol 22:3099-3108.
4. 1 adrenoceptors by fluorescent ligand binding and image analysis reveals identical binding properties of clustered and diffuse populations of receptors. J Pharmacol Exp Ther 286:984-990.
5. 11α induced by agonist stimulation. Biochem J 340:529-538.
6. 1B-adrenergic receptor subcellular distribution and function. J Biol Chem 270:8902-8909.
7. Ford APDW, Arredondo NF, Blue DR Jr, Bonhaus DW, Jasper J, Kava MS, Lesnick J, Pfister JR, Shieh IA, Viniont RL, Williams TJ, McNeal JE, Stamey TA and Clarke DE (1996) RS-17053 (N-[2-(2-cyclopropylmethoxyphenoxyiethyl]-5-chloro-alpha, alpha-dimethyl-1H-indole-3-ethanamine hydrochloride), a selective alpha(1A)-adrenoceptor antagonist, displays low affinity for functional alpha1-adrenoceptors in human prostate: Implications for adrenoceptor classification. Mol Pharmacol 49:209-215
8. Ford APDW, Chang DJ, Clarke DE, Daniels DV, Eglen RM, Gever JR, Jasper JR, Kava MS, Lachnit WG, Lesnick JD, Meloy TD, Stepan GT and Williams TJ (1998) Alpha-1A-versus alpha-1L-adrenoceptors: A pharmacological comparison. Pharmacol Toxicol Suppl 83:12-14.
9. Gründemann D, Schechinger B, Rappold GA and Schömig E (1998) Molecular identification of the corticosterone-sensitive extraneuronal catecholamine transporter. Nat Neurosci 1:349-351.
10. Hall RA, Premont RT and Lefkowitz RJ (1999) Heptahelical receptor signaling: Beyond the G protein paradigm. J Cell Biol 145:927-932.
11. Hieble JP, Bylund DB, Clarke DE, Eikenburg DC, Langer SZ, Lefkowitz RJ, Minneman KP and Ruffolo RR Jr (1995) International Union of Pharmacology X: Recommendations for nomenclature of α1-adrenoceptors: Consensus update. Pharmacol Rev 47:267-270.
12. 1-adrenoceptors irrespective of the subtype. Mol Pharmacol 52:764-770.
13. 1-adrenoceptor subtypes. FEBS Lett 386: 141-148.
14. 2+ stores in single smooth muscle cells from rat cerebral arteries. J Physiol (Lond) 501:497-508.
15. 1A-adrenoceptor characterized in rat cerebral cortex and vas deferens. Br J Pharmacol 111:1003-1308.
16. Marshall I, Burt RP and Chapple CR (1995) Noradrenaline contractions of human prostate mediated by alpha(1A)-alpha(1c-) adrenoceptor subtypes. Br J Pharm 115:781-786.
17. McGrath JC, Arribas S and Daly CJ (1996a) Fluorescent ligands for the study of receptors. Trends Pharmacol Sci 17:393-399.
18. McGrath JC, Lepor H and Wyllie MG (1996b) Report of a unique meeting between the alpha-blocker subcommittee and the pharmaceutical industry. Urology 48: 665-667.
19. Schwinn DA, Lomasney JW, Lorenz W, Szklut PJ, Fremeau RT Jr, Yang-Feng TL, Caron MG, Lefkowitz RJ and Cotecchia S (1990) Molecular cloning and expression of the cDNA for a novel alpha1-adrenergic receptor subtype. J Biol Chem 265: 8183-8189.
20. Slice LW, Yee HF Jr and Walsh JH (1998) Visualization of internalization and recycling of the gastrin releasing peptide receptor-green fluorescent protein chimera expressed in epithelial cells. Receptors Channels 6:201-212.
21. Smith TJ, Jennings TA, Sciaky D and Cao HJ (1999) Prostaglandin-endoperoxidase H synthase-2 expression in human thyroid epithelium. J Biol Chem 274:15622-15632.
22. Takeda M, Hatano A, Komeyama T, Koizumi T, Mizusawa T, Kanai T, Tomita Y, Maruyama Y and Nagatomo T (1997) Alpha-1 adrenoceptor subtypes (high, low) in Human benign prostatic hypertrophy tissue according to affinities for prazosin. Prostate 31:216-222.
23. 1A-adrenoceptor selective antagonists. Br J Pharmacol 127:252-258.