Mutation of tyrosine in the conserved NPXXY sequence leads to constitutive phosphorylation and internalization, but not signaling, of the human B 2 bradykinin receptor

Journal of Biological Chemistry

Volumn 279, Issue 30, 2004, Pages 31268-31276

  Kalatskaya, Irina ^a   Schüssler, Steffen ^a   Blaukat, Andree ^b   Müller Esterl, Werner ^c   Jochum, Marianne ^a   Proud, David ^d   Faussner, Alexander ^a  

^a UNIVERSITY OF MUNICH   (Germany)

^b UNIVERSITY OF HEIDELBERG   (Germany)

^c GOETHE UNIVERSITY   (Germany)

^d UNIVERSITY OF CALGARY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVATION ANALYSIS; ANTIBODIES; CELL MEMBRANES; FLUORESCENCE; GENES; IMMUNOLOGY; PRECIPITATION (CHEMICAL); PROTEINS; REACTION KINETICS;

CONFOCAL FLUORESCENCE MICROSCOPY; IMMUNOPRECIPITATION; MUTATED RECEPTORS; RECEPTOR INTERNALIZATION;

MUTAGENESIS;

ALANINE; AMINO ACID; ANTIBODY; BETA ADRENERGIC RECEPTOR; BRADYKININ B2 RECEPTOR; G PROTEIN COUPLED RECEPTOR; GREEN FLUORESCENT PROTEIN; GUANINE NUCLEOTIDE BINDING PROTEIN; INOSITOL PHOSPHATE; LIGAND; MUTANT PROTEIN; PHOSPHOPEPTIDE; RHODAMINE; RHODOPSIN; TYROSINE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ANTIBODY LABELING; ARTICLE; CHIMERA; CONFOCAL MICROSCOPY; CONTROLLED STUDY; CYTOSOL; EMBRYO; GENETIC CONSERVATION; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; INTERNALIZATION; LIGAND BINDING; MUTATION; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION;

AMINO ACID SEQUENCE; CELL LINE; CONSERVED SEQUENCE; GTP-BINDING PROTEIN ALPHA SUBUNITS, GQ-G11; HUMANS; KINETICS; LIGANDS; MUTAGENESIS, SITE-DIRECTED; PHOSPHORYLATION; PROTEIN CONFORMATION; RECEPTOR, BRADYKININ B2; RECOMBINANT FUSION PROTEINS; SIGNAL TRANSDUCTION; TYROSINE;

EID: 3843130785     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M401796200     Document Type: Article

References (28)

1. Gether, U. (2000) Endrocr. Rev. 21, 90-113
2. Frederikson, R., Lagerström, M. C., Lundin, L.-G., and Schiöth, H. B. (2003) Mol. Pharmacol. 63, 1256-1272
3. Fritze, O., Filipek, S., Kuksa, V., Palczewski, K., Hofmann, K. P., and Ernst, O. P. (2003) Proc. Natl. Acad. Sci. U. S. A. 100, 2290-2295
4. Bouley, R., Sun, T.-X., Chenard, M., McLaughlin, M., McKee, M., Lin, H. Y., Brown, D., and Ausiello, D. A. (2003) Am. J. Physiol. Cell Physiol. 285, C750-C762
5. Prioleau, C., Visiers, I., Ebersole, B. J., Weinstein, H., and Sealfon, S. C. (2002) J. Biol. Chem. 277, 36577-36584
6. Rosendorff, A., Ebersole, B. J., and Sealfon, S. C. (2000) Mol. Brain Res. 84, 90-96
7. Hukovic, N., Panetta, R., Kumar, U., Rocheville, M., and Patel, Y. C. (1998) J. Biol. Chem. 273, 21416-21422
8. Wang, J., Zheng, J., Anderson, J. L., and Toews, M. L. (1997) Mol. Pharmacol. 52, 306-313
9. Böhm, S. K., Khitin, L. M., Smeekens, S. P., Grady, E. F., Payan, D. G., and Bunnett, N. W. (1997) J. Biol. Chem. 272, 2363-2372
10. Gabilondo, A. M., Krasel, C., and Lohse, M. J. (1996) Eur. J. Pharmacol. 307, 243-250
11. Ferguson, S. S. G., Menard, L., Barak, L. S., Koch, W. J., Colapietro, A. M., and Caron, M. G. (1995) J. Biol. Chem. 270, 24782-24789
12. Hunyady, L., Bor, M., Baukal, A. J., Balla, T., and Catt, K. J. (1995) J. Biol. Chem. 270, 16602-16609
13. Barak, L. S., Tiberi, M., Freedman, N. J., Kwatra, M. M., Lefkowitz, R. J., and Caron, M. G. (1994) J. Biol. Chem. 269, 2790-2795
14. Regoli, D., and Barabe, L. (1988) Methods Enzymol. 163, 210-230
15. Proud, D., and Kaplan, A. P. (1988) Annu. Rev. Immunol. 6, 4-83
16. Bhoola, K. D., Figueroa, C. D., and Worthy, K. (1992) Pharmacol. Rev. 44, 1-80
17. Blaukat, A., Pizard, A., Breit A., Wernstedt, C., Alhenc-Gelas, F., Müller-Esterl, W., and Dikic, I. (2001) J. Biol. Chem. 276, 40431-40440
18. Ballesteros, J. A., and Weinstein, H. (1995) Methods Neurosci. 25, 366-428
19. Faussner, A., Bauer, A., Kalatskaya, I., Jochum, M., and Fritz, H. (2003) Am. J. Physiol. 284, H1909-H1916
20. Hess, J. F., Borkowski, J. A., Young, G. S., Strader, C. D., and Ransom, R. W. (1992) Biochem. Biophys. Res. Commun. 184, 260-268
21. Blaukat, A., AbdAlla, S., Lohse, M. J., and Müller-Esterl, W. (1996) J. Biol. Chem. 271, 32366-32374
22. Boyle, W. J., van der Geer, P., and Hunter, T. (1991) Methods Enzymol. 201, 110-149
23. Faussner, A., Heinz-Erian, P., Klier, C., and Roscher, A. A. (1991) J. Biol. Chem. 266, 9442-9446
24. Sabourin, T., Bastien, L., Bachvarov, D. R., and Marceau, F. (2002) Mol. Pharmacol. 61, 546-553
25. Marchese, A., Chen, C., Kim, Y. M., and Benovic, J. L. (2003) Trends Biochem. Sci. 28, 369-376
26. Trowbridge, I. S., Collawn, J. F., and Hopkins, C. R. (1993) Annu. Rev. Cell Biol. 9, 129-161
27. Samama, P., Cotecchia, S., Costa, T., and Lefkowitz, R. J. (1993) J. Biol. Chem. 268, 4625-4638
28. Milligan G. (2003) Trends Pharmacol. Sci. 24, 87-90