Exploring the dynamics of regulation of G protein-coupled receptors using green fluorescent protein

British Journal of Pharmacology

Volumn 128, Issue 3, 1999, Pages 501-510

  Milligan, Graeme ^a  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

Arrestins; G protein; G protein coupled receptor; Green fluorescent protein; Imaging

Indexed keywords

ALPRENOLOL; BETA 2 ADRENERGIC RECEPTOR; BETAXOLOL; CELL RECEPTOR; GREEN FLUORESCENT PROTEIN; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE TRIPHOSPHATE; ISOPRENALINE; RETINA S ANTIGEN;

BINDING AFFINITY; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; RECEPTOR AFFINITY; RECEPTOR BINDING; REVIEW;

GREEN FLUORESCENT PROTEINS; GTP-BINDING PROTEINS; HUMANS; LUMINESCENT PROTEINS; RECEPTORS, CELL SURFACE; RECOMBINANT FUSION PROTEINS;

EID: 0032699862     PISSN: 00071188     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.bjp.0702824     Document Type: Review

References (51)

1. ASHWORTH, R., YU, R., NELSON, E.J., DERMER, S., GERSHENGORN, M.C. & HINKLE, P.M. (1995). Visualization of the thyrotropin-releasing hormone receptor and its ligand during endocytosis and recycling. Proc. Natl. Acad. Sci. U.S.A., 92, 512-516.
2. 1b-adrenoceptor with green fluorescent protein. Mol. Endocrinol., 12, 1099-1111.
3. BARAK, L.S., FERGUSON, S.S., ZHANG, J., MARTENSON, C., MEYER, T. & CARON, M.G. (1997). Internal trafficking and surface mobility of a functionally intact beta2-adrenergic receptor-green fluorescent protein conjugate. Mol. Pharmacol., 51, 177-184.
4. BARLIC, J., KHANDAKER, M.H., MAHON, E., ANDREWS, J., DEVRIES, M.E., MITCHELL, G.B., RAHIMPOUR, R., TAN, C.M., FERGUSON, S.S.G. & KELVIN, D.J. (1999). β-arrestins regulate interleukin-8-induced CXCR1 internalization. J. Biol. Chem., 274, 16287-16294.
5. BOURNE, H. (1997). How receptors talk to trimeric G proteins. Curr. Opin. Cell Biol., 9, 134-142.
6. CHALFIE, M. & KAIN, S.R. (ed.). (1998). Green fluorescent protein properties, applications and protocols. 385pp. Wiley-Liss Press, New York.
7. CHEN, W., SHIELDS, T.S., STORK, P.J.S. & CONE, R.D. (1995). A colorimetric assay for measuring activation of Gs-and Gq-coupled signaling pathways. Anal. Biochem., 226, 349-354.
8. CLAGUE, M.J. (1998). Molecular aspects of the endocytic pathway. Biochem. J., 336, 271-282.
9. ™ high-content screening system. J. Biomol. Screening, 4, 75-86.
10. CVEJIC, S. & DEVI, L.A. (1997). Dimerization of the δ opioid receptor. Implication for a role in receptor internalization. J. Biol. Chem., 272, 26959-26964.
11. 1-adrenoceptors by fluorescent binding and image analysis reveals identical binding properties of clustered and diffuse populations of receptors. J. Pharmacol. Exp. Ther., 268, 984-990.
12. DERY, O., THOMA, M.S., WONG, H., GRADY, E.F. & BUNNETT, N.W. (1999). Trafficking of protease-activated receptor-2 and β-arrestin-1 tagged with green fluorescent protein. β-arrestin-dependent endocytosis of a proteinase receptor. J. Biol. Chem., 274, 18524-18535.
13. DIVIANI, D., LATTION, A.L. & COTECCHIA, S. (1997). Characterization of the phosphorylation sites involved in G protein-coupled receptor kinase-and protein kinase C-mediated desensitization of the alpha1B-adrenergic receptor. J. Biol. Chem., 272, 28712-28719.
14. DRMOTA, T., GOULD, G.W. & MILLIGAN, G. (1998a). Real time visualization of agonist-mediated redistribution and internalisation of a green fluorescent protein-tagged form of the thyrotropin-releasing receptor. J. Biol. Chem., 273, 24000-24008.
15. 11α induced by agonist stimulation. Biochem. J., 340, 529-538.
16. 11α and thyrotropin releasing hormone (TRH) receptors proceed on different time scales. J. Biol. Chem., 273, 21699-21707.
17. ELLENBERG, J., LIPPINCOTT-SCHWARTZ, J. & PRESLEY, J.F. (1999). Dual-colour imaging with GFP variants. Trends Cell Biol., 9, 52-56.
18. FERGUSON, S.S. (1998). Using green fluorescent protein to understand the mechanisms of G-protein-coupled receptor regulation. Braz. J. Med. Biol. Res., 31, 1471-1477.
19. FERGUSON, S.S., ZHANG, J., BARAK, L.S. & CARON, M.G. (1998). Molecular mechanisms of G protein-coupled receptor desensitization and resensitization. Life Sci., 62, 1561-1565.
20. FERRER, J.C., BAQUE, S. & GUINOVART, J.J. (1997). Muscle glycogen synthase translocates from the cell nucleus to the cystosol in response to glucose. FEBS Lett., 415, 249-252.
21. 11α is sufficient to target a green fluorescent protein reporter to caveolin-enriched plasma domains. Palmitoylation of caveolin-1 is required for the recognition of dually acylated G-protein α subunits in vivo. J. Biol. Chem., 274, 5843-5850.
22. 2+ activation of the human calcium sensing receptor. A study with receptor-green fluorescent protein fusions. J. Biol. Chem., 273, 29712-29718.
23. GROARKE, D.A., WILSON, S., KRASEL, C. & MILLIGAN, G. (1999). Visualization of agonist-induced association and trafficking of green fluorescent protein-tagged forms of both β-arrestin-I and the thyrotropin releasing hormone receptor-1. J. Biol. Chem. (in press).
24. GROS, R., BENOVIC, J.L., TAN, C.M. & FELDMEN, R.D. (1997). G-protein-coupled receptor kinase activity is increased in hypertension. J. Clin. Invest., 99, 2087-2093.
25. HAAS, J., PARK, E.C. & SEED, B. (1996). Codon usage limitation in the expression of HIV-I envelope glycoprotein. Curr. Biol., 6, 315-324.
26. + exchanger regulatory factor family of PDZ proteins. Proc. Natl. Acad. Sci. U.S.A., 95, 8496-8501.
27. 2-adrenergic receptor transmembrane domain inhibits both receptor dimerization and activation. J. Biol. Chem., 271, 16384-16392.
28. 1-adrenoceptors irrespective of the subtype. Mol. Pharmacol., 52, 764-770.
29. IACCARINO, G., DOLBER, P.C., LEFKOWITZ, R.J. & KOCH, W.J. (1999). beta-adrenergic receptor kinase-1 levels in catecholamine-induced myocardial hypertrophy: regulation by beta-but not alpha 1-adrenergic stimulation. Hypertension, 33, 396-401.
30. IACCARINO, G., TOMHAVE, E.D., LEFKOWITZ, R.J. & KOCH, W.J. (1998). Reciprocal in vivo regulation of myocardial G protein-coupled receptor kinase expression by beta-adrenergic receptor stimulation and blockade. Circulation, 98, 1783-1789.
31. KALLAL, L., GAGNON, A.W., PENN, R.B. & BENOVIC, J.L. (1998). Visualization of agonist-induced sequestration and down-regulation of a green fluorescent protein-tagged beta2-adrenergic receptor. J. Biol. Chem., 273, 322-328.
32. KOENIG, J.A. & EDWARDSON, J.M. (1997). Endocytosis and recycling of G protein-coupled receptors. Trends Pharmacol. Sci., 18, 276-287.
33. LUTTRELL, L.M., FERGUSON, S.S., DAAKA, Y., MILLER, W.E., MAUDSLEY, S., DELLA ROCCA, G.J., LIN, F., KAWAKATSU, H., OWADA, K., LUTTRELL, D.K., CARON, M.G. & LEFKOWITZ, R.J. (1999). Beta-arrestin-dependent formation of beta2 adrenergic receptor-Src protein kinase complexes. Science, 283, 655-661.
34. MCCONALOGUE, K., DERY, O., LOVETT, M., WONG, H., WALSH, J.H., GRADY, E.F. & BUNNETT, N.W. (1999). Substance P-induced trafficking of β-arrestins. The role of β-arrestins in endocytosis of the neurokinin-1 receptor. J. Biol. Chem., 274, 16257-16268.
35. MCGRATH, J.C., ARRIBAS, S. & DALY, C.J. (1996). Fluorescent ligands for the study of receptors. Trends Pharmacol. Sci., 17, 393-399.
36. MCLEAN, A.J. & MILLIGAN, G, (1999). Receptor-GFP fusion proteins; A study of receptor internalisation and trafficking. In: Thiery, J.P. (ed.). Molecular Mechanisms of Transcellular Signalling: From Membrane Receptors to Transcription Factors. NATO Science Series. A.S.I. Series A: pp. 247-252.
37. MHAOUTY-KODJA, S., BARAK, L.S., SCHEER, A., ABUIN, L., DIVIANI, D., CARON, M.G. & COTECCHIA, S. (1999). Constitutively active alpha-1b adrenergic receptor mutants display different phosphorylation and internalization features. Mol. Pharmacol., 55, 339-347.
38. MILLIGAN, G. (1998). New aspects of G protein-coupled receptor signaling and regulation. Trends Endocrinol. Metabolism, 9, 13-19.
39. MILLIGAN, G., GROARKE, D.A., MCLEAN, A., WARD, R., FONG, C.W., CAVALLI, A. & DRMOTA, T. (1998). Diversity in the signaling and regulation of G protein-coupled receptors. Biochem. Soc. Trans., 27, 149-154.
40. MILLIGAN, G. & REES, S. (1999). Chimaeric Gα proteins: their potential use in drug discovery. Trends Pharmacol. Sci., 20, 118-124.
41. PETIOT, A., OGIER-DENIS, E., BAUVY, C., CLUZEAUD, F., VANDEWALLE, A. & CODOGNO, P. (1999). Subcellular localization of the Gi3 protein and G alpha interacting protein, two proteins involved in the control of macroautophagy in human colon cancer HT-29 cells. Biochem. J., 337, 289-295.
42. POLLOCK, B.A. & HEIM, R. (1999). Using GFP in FRET-based applications. Trends Cell Biol., 9, 57-60.
43. ROCKMAN, H.A., CHOI, D.J., AKHTER, S.A., JABER, M., GIROS, B., LEFKOWITZ, R.J., CARON, M.G. & KOCH, W.J. (1998). Control of myocardial contractile function by the level of beta-adrenergic receptor kinase 1 in gene-targeted mice. J. Biol. Chem., 273, 18180-18184.
44. SCHULEIN, R., HERMOSILLA, R., OKSCHE, A., DEHE, M., WIESNER, B., KRAUSE, G. & ROSENTHAL, W. (1998). A dileucine sequence and an upstream glutamate residue in the intracellular carboxyl terminus of the vasopressin V2 receptor are essential for cell surface transport in COS.M6 cells. Mol. Pharmacol., 54, 525-535.
45. TARASOVA, N.I., STAUBER, R.H., CHOI, J.K., HUDSON, E.A., CZERWINSKI, G., MILLER, J.L., PAVLAKIS, G.N., MICHEJDA, C.J. & WANK, S.A. (1997). Visualization of G protein-coupled receptor trafficking with the aid of the green fluorescent protein. Endocytosis and recycling of cholecystokinin receptor type A. J. Biol. Chem., 272, 14817-14824.
46. VRECL, M., ANDERSON, L., HANYALOGLU, A., MCGREGOR, A.M., GROARKE, A.D., MILLIGAN, G., TAYLOR, P.L. & EIDNE, K.A. (1998). Agonist-induced endocytosis and recycling of the gonadotropin-releasing hormone (GnRH) receptor: Effect of β-arrestin on internalization kinetics. Mol. Endocrinol. 12, 1818-1829.
47. WANG, S. & HAZELRIGG, T. (1994). Implications for bed mRNA localization from spatial distribution of exu protein in Drosophila oogenesis. Nature, 369, 400-403.
48. XU, Y., PISTON, D.W. & JOHNSON, C.H. (1999). A bioluminescence resonance energy transfer (BRET) system: Application to interacting circadian clock proteins. Proc. Natl. Acad. Sci. U.S.A., 96, 151-156.
49. ZHANG, J., BARAK, L.S., ANBORGH, P.H., LAPORTE, S.A., CARON, M.G. & FERGUSON, S.S.G. (1999). Cellular trafficking of G protein-coupled receptor/β-arrestin endocytic complexes. J. Biol. Chem., 274, 10999-11006.
50. ZHANG, J., FERGUSON, S.S., BARAK, L.S., ABER, M.J., GIROS, B., LEFKOWITZ, R.J. & CARON, M.G. (1998a). Molecular mechanisms of G protein-coupled receptor signaling: role of G protein-coupled receptor kinase and arrestins in receptor desensitization and resensitization. Receptors Channels, 5, 193-199.
51. ZHANG, J., FERGUSON, S.S., BARAK, L.S., BODDULURI, S.R., LAPORTE, S.A., LAW, P.Y. & CARON, M.G. (1998b). Role for G protein-coupled receptor kinase in agonist-specific regulation of mu-opioid receptor responsiveness. Proc. Natl. Acad. Sci. U.S.A., 95, 7157-7162.