Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase

Nature Methods

Volumn 2, Issue 2, 2005, Pages 99-104

  Chen, Irwin ^a   Howarth, Mark ^a   Lin, Weiying ^a   Ting, Alice Y ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; BACTERIAL ENZYME; BENZOPHENONE; BIOTIN; BIOTIN LIGASE; CELL SURFACE PROTEIN; EPIDERMAL GROWTH FACTOR RECEPTOR; FLUORESCEIN; HYDRAZIDE; HYDROXYLAMINE; KETONE; LIGASE; PEPTIDE; UNCLASSIFIED DRUG; BIRA PROTEIN, E COLI; ESCHERICHIA COLI PROTEIN; FLUORESCENT DYE; MEMBRANE PROTEIN; REPRESSOR PROTEIN; TRANSCRIPTION FACTOR;

ARTICLE; CELL LYSATE; CELL SURFACE; CONTROLLED STUDY; ENZYME SPECIFICITY; ESCHERICHIA COLI; HELA CELL; HUMAN; HUMAN CELL; METHODOLOGY; MOLECULAR PROBE; PHOTOAFFINITY LABELING; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN LABELING; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; BIOTINYLATION; CELL LINE; EVALUATION; FLUORESCENCE MICROSCOPY; GENETIC PROCEDURES; KIDNEY; METABOLISM; SENSITIVITY AND SPECIFICITY; STAINING;

ESCHERICHIA; ESCHERICHIA COLI; MAMMALIA;

BIOTINYLATION; CARBON-NITROGEN LIGASES; CELL LINE; ESCHERICHIA COLI PROTEINS; FLUORESCENT DYES; HELA CELLS; HUMANS; KIDNEY; MEMBRANE PROTEINS; MICROSCOPY, FLUORESCENCE; MOLECULAR PROBE TECHNIQUES; RECEPTOR, EPIDERMAL GROWTH FACTOR; REPRESSOR PROTEINS; SENSITIVITY AND SPECIFICITY; STAINING AND LABELING; TRANSCRIPTION FACTORS;

EID: 18744401100     PISSN: 15487091     EISSN: None     Source Type: Journal    
DOI: 10.1038/nmeth735     Document Type: Article

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